IN VIVO OPTICAL DIAGNOSIS OF ORAL MALIGNANCY IN HUMAN SUBJECTS

人类受试者口腔恶性肿瘤的体内光学诊断

• 批准号: 8362606
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362606
• 关键词: Biopsy; Biotechnology; Blinded; Blood Vessels; Cellular Structures; Cessation of life; Collagen; Data; Detection; Diagnosis; Diagnostic; Diagnostic Imaging; Dysplasia; Ensure; Epithelial; Fluorescence; Funding; Grant; Histopathology; Image; Lasers; Malignant - descriptor; Malignant Neoplasms; Maps; Methods; Modality; National Center for Research Resources; Optical Coherence Tomography; Optics; Oral Diagnosis; Pathology; Patients; Principal Investigator; Quality of life; ROC Curve; Research; Research Infrastructure; Research Personnel; Resources; Screening for cancer; Sensitivity and Specificity; Site; Source; Specificity; Structure; Surface; Techniques; Technology; Testing; Tissues; United States National Institutes of Health; cost; human subject; in vivo; malignant mouth neoplasm; multi-photon; oral dysplasia; oral lesion; oral premalignancy; oral tissue; second harmonic; statistics; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objectives: Early detection of cancer and its curable precursors remains the best way to ensure patient survival and quality of life. Despite significant advances in treatment, oral cancer still results in 10,000 U.S. deaths annually, mainly due to the late detection of most oral lesions. Specific aim: Using a combination of non-invasive optical in vivo technologies to test a multi-modality approach to non-invasive diagnostics of oral premalignancy and malignancy in human subjects. Methods: In 30 patients with healthy, dysplastic, and/or malignant oral tissues, in vivo optical coherence tomography (OCT) and optical Doppler tomography (ODT) mapped epithelial, subepithelial and vascular change in specific, marked sites prior to routine biopsy. Ex vivo multi-wavelength multi-photon (MPM) and second harmonic generated (SHG) fluorescence techniques provided parallel data on surface and subsurface tissue structure, specifically collagen presence and structure, cellular presence, and vasculature. Images were diagnosed by 2 blinded, pre-standardized investigators using a standardized scale from 0-6 for all modalities. Histopathological sections were prepared and pathology evaluated on a scale of 0-6. ANOVA techniques compared imaging diagnostics with histopathology. 95% confidence limits of the sensitivity and specificity were established for the diagnostic capability of OCT/ODT+ MPM/SHG using ROC curves and kappa statistics. Results: Imaging data were reproducibly obtained with good accuracy. Dysplasia- and malignancy-related structural and vascular changes were clearly visible to tissue depths of 2mm. Sensitivity (OCT/ODT alone: 72-89%; OCT+MPM/SHG: 76-90%) and specificity (OCT alone: 68-84%;OCT+MPM/SHG: 74-88%) compared well with conventional techniques. Conclusions: OCT/ODT and MPM/SHG are promising non-invasive in vivo diagnostic modalities for oral dysplasia and malignancy.

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