OPTICAL AND MOLECULAR APPROACHES TO THE STUDY OF CHEMICAL AGENTS

研究化学试剂的光学和分子方法

• 批准号: 8362624
• 负责人: TATIANA B KRASIEVA
• 金额: $ 1.21万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362624
• 关键词: 2-chloroethyl ethyl sulfide; Adhesions; Biosensor; Biotechnology; Cell Cycle; Cell physiology; Cellular Structures; Chemical Agents; Cytoskeleton; Development; Event; Exposure to; Funding; Future; Goals; Grant; Knowledge; Lasers; Molecular; Mono-S; Mustard; Mustard Gas; National Center for Research Resources; Optics; Principal Investigator; Research; Research Infrastructure; Resources; Role; Source; Tissues; United States National Institutes of Health; Wound Healing; cost; effective therapy; treatment strategy

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of this project is to apply modern optical and molecular/cellular probes to study SM effects on cell structure and function by using 2-chloroethyl ethyl sulfide, a mono-functional sulfur mustard (CEES). This research effort is intended to enhance our understanding of the primary cellular effects of SM exposure. To this end, this fundamental knowledge of the cellular and molecular events underlying SM exposure will serve as a keystone to future biosensor/probe development and will facilitate the development of more effective treatment strategies. The specific objectives of this project are: (1) to examine the effects of CEES (half-mustard) at the molecular and cellular level, specifically, on the cytoskeleton in relationship to the cell cycle, (2) to determine a potential role for Src in the disorganization of the cytoskeleton and adhesion following exposure to CEES, (3) to study the effects of CEES at the tissue level as related to wound healing.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本项目的总体目标是应用现代光学和分子/细胞探针，通过使用2-氯乙基乙基硫醚（一种单功能硫芥（CEES））来研究SM对细胞结构和功能的影响。这项研究工作的目的是提高我们对SM暴露的主要细胞效应的理解。为此，SM暴露的细胞和分子事件的基本知识将作为未来生物传感器/探针开发的基石，并将促进更有效的治疗策略的开发。该项目的具体目标是：（1）在分子和细胞水平上检查CEES（半芥子气）的影响，特别是与细胞周期相关的细胞骨架，（2）确定Src在接触CEES后细胞骨架和粘附的解体中的潜在作用，（3）研究CEES在组织水平上与伤口愈合相关的影响。