PREVENTING RADIATION DECAY IN PROTEIN CRYSTALS

防止蛋白质晶体的辐射衰变

• 批准号: 8362093
• 负责人: IRIMPAN I MATHEWS
• 金额: $ 2.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362093
• 关键词: Address; Belief; Data Collection; Funding; Gases; Generations; Grant; National Center for Research Resources; Photons; Principal Investigator; Procedures; Proteins; Radiation; Research; Research Infrastructure; Research Personnel; Resources; Solvents; Source; Synchrotrons; Temperature; United States National Institutes of Health; cost; prevent; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The high-flux beam lines at third generation synchrotrons produce very high intensity x-radiation. Unfortunately, due to decay of the protein crystals in the high intensity beam, the synchrotrons users insert attenuators to reduce the beam intensity. Therefore, beam lines are not utilizing their full potential for protein diffraction experiments. There have been several attempts to study the mechanisms of protein crystal decay. The common belief is that the radiation decay is partly due to the effect of the radicals produced by the interaction of x-ray photons with the protein or solvent atoms in the crystal. Researchers have used various procedures like lowering the data collection temperature, incorporating radical scavengers, etc. to reduce the effect of these radicals. The current project tries to address the radiation decay of protein crystals by employing few selected gases as radical scavengers.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 第三代同步基因的高频梁线产生非常高的强度X辐射。不幸的是，由于高强度束中蛋白质晶体的衰减，同步性使用者插入衰减器以降低束强度。 因此，光束线没有利用其全部潜力进行蛋白质衍射实验。 已经有几次尝试研究蛋白质晶体衰减的机制。 普遍的信念是，辐射衰减部分是由于X射线光子与晶体中蛋白质或溶剂的相互作用所产生的自由基的作用。 研究人员使用了各种程序，例如降低数据收集温度，结合了自由基清除剂等，以降低这些激进分子的效果。当前的项目试图通过使用几乎选择的气体作为根部清除剂来解决蛋白质晶体的辐射衰减。