FUNCTIONAL STUDY OF ADP-GLUCOSE PYROPHOSPHORYLASE

ADP-葡萄糖焦磷酸酶的功能研究

• 批准号: 8362108
• 负责人: IRIMPAN I MATHEWS
• 金额: $ 0.44万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362108
• 关键词: Anabolism; Bacteria; Binding; Carbon; Environment; Funding; Glucose; Glucose-1-phosphate adenylyltransferase; Glycogen; Grant; National Center for Research Resources; Organism; Pathway interactions; Plants; Polysaccharides; Principal Investigator; Radiation; Reaction; Regulation; Research; Research Infrastructure; Resources; Site; Source; Starch; Starvation; United States National Institutes of Health; coping; cost; insight; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. ADP-glucose pyrophosphorylase catalyzes the first rate-limiting step in starch biosynthesis in plants and glycogen biosynthesis in bacteria. It is the enzymatic site for regulation of storage polysaccharide accumulation in plants and bacteria, being allosterically activated or inhibited by metabolites. The accumulation of alpah-1,4-polyglucans is an important strategy to cope with transient starvation conditions in the environment. In bacteria and plants, the synthesis of glycogen and starch occurs by utilizing ADP-glucose as the glucosyl donor for elongation of the alpha-1,4-glucosidic chain. The main regulatory step takes place at the level of ADP-glucose synthesis, a reaction catalyzed by ADP-Glc pyrophosphorylase (PPase). Most of the ADP-Glc PPases are allosterically regulated by intermediates of the major carbon assimilatory pathway in the organism. ADP-Glc PPase bound with various compounds will be crystallized and structural studies will be performed to gain structural and functional insight.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 ADP-葡萄糖焦磷酸化酶在植物中催化淀粉生物合成的第一个限速步骤，在细菌中催化糖原生物合成。它是调节植物和细菌中储存的多糖积累的酶场所，被代谢产物变构激活或抑制。Alpah-1，4-葡聚糖的积累是应对环境中短暂饥饿条件的重要策略。在细菌和植物中，糖原和淀粉的合成是通过利用ADP-葡萄糖作为葡萄糖供体来延长α-1，4-葡萄糖苷链的。主要的调控步骤发生在ADP-葡萄糖合成水平，这是一个由ADP-Glc焦磷酸酶(PPase)催化的反应。大多数ADP-Glc PPase受生物体内主要碳同化途径的中间体的变构调节。与各种化合物结合的ADP-Glc PPase将被结晶，并将进行结构研究以获得结构和功能方面的信息。