FUNCTIONAL STUDY OF ADP-GLUCOSE PYROPHOSPHORYLASE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 ADP-葡萄糖焦磷酸化酶催化植物淀粉生物合成和细菌糖原生物合成的第一个限速步骤。 它是调节植物和细菌中贮藏多糖积累的酶位点，被代谢物变构激活或抑制。 α-1，4-葡聚糖的积累是科普环境中短暂饥饿条件的重要策略。在细菌和植物中，糖原和淀粉的合成通过利用ADP-葡萄糖作为用于延长α-1，4-糖苷链的葡糖基供体而发生。 主要的调节步骤发生在ADP-葡萄糖合成水平，这是由ADP-Glc焦磷酸化酶（PPase）催化的反应。 大多数ADP-Glc PP酶受生物体中主要碳同化途径的中间体的别构调节。 将与各种化合物结合的ADP-Glc PPase结晶，并进行结构研究以获得结构和功能见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. ADP-glucose pyrophosphorylase catalyzes the first rate-limiting step in starch biosynthesis in plants and glycogen biosynthesis in bacteria. It is the enzymatic site for regulation of storage polysaccharide accumulation in plants and bacteria, being allosterically activated or inhibited by metabolites. The accumulation of alpah-1,4-polyglucans is an important strategy to cope with transient starvation conditions in the environment. In bacteria and plants, the synthesis of glycogen and starch occurs by utilizing ADP-glucose as the glucosyl donor for elongation of the alpha-1,4-glucosidic chain. The main regulatory step takes place at the level of ADP-glucose synthesis, a reaction catalyzed by ADP-Glc pyrophosphorylase (PPase). Most of the ADP-Glc PPases are allosterically regulated by intermediates of the major carbon assimilatory pathway in the organism. ADP-Glc PPase bound with various compounds will be crystallized and structural studies will be performed to gain structural and functional insight.