TWO-LASER FPOP AND PROTEIN FOLDING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 利用FPOP作为一种快速标记策略的优势，我们正在建立一个双激光系统，该系统利用Nd：YAG激光和拉曼移位器来快速加热蛋白质，然后使用第二个激光在展开过程中产生自由基来足迹蛋白质。这种方法将使我们能够在微秒的时间尺度上探测蛋白质的动力学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Taking advantage of FPOP as a fast labeling strategy, we are building a two laser system that utilizes a Nd:YAG laser and a Raman shifter to quickly heat a protein, and then use the second laser to generate radicals to footprint the protein during unfolding. This method will allow us to probe protein dynamics on a microsecond time scale.