HIGH ENERGY DIFFRACTION DEVELOPMENT

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 小角度X射线纤维衍射是从工作肌肉中获得结构信息和生理信息的首选方法。进展的一个重要障碍是肌肉对射束线18 ID处典型的高X射线剂量率下的辐射损伤的敏感性。 可用于减轻辐射损伤的主要策略是使用相对较大的样品并在曝光期间在光束中对其进行扫描。这是不可能的小样本，如那些分离的啮齿动物的心脏，所以一些其他的方法来减少损害显然是必要的。来自日本Spring-8研究人员的轶事信息表明，活肌肉在15 keV下的寿命比在12 keV下长得多，但这在同行评审文献中尚未记录。在这里，我们探讨了使用18 ID在15 keV下进行肌肉实验的可能性，并观察这是否对样品寿命有明显影响

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Small-angle x-ray fiber diffraction is the method of choice to obtain structural information at the same time as physiological information from working muscle. A significant barrier to progress is the susceptibility of muscle to radiation damage at the high x-ray dose rates typical at beamline 18ID. The main strategy available to mitigate radiation damage is to use a relatively large sample and scan it in the beam during the exposure. This isn't possible with small samples such as those isolated form the hearts of rodents so some other way of reducing damage is clearly needed. Anecdotal information from researchers at Spring-8 in Japan have indicated that living muscles last much longer at 15 keV than at 12 keV but this has not been documented in the peer reviewed literature. Here we explored the possibility of using 18 ID to do muscle experiments at 15 keV and to see if this had appreciable effects on sample lifetimes