ANALYTICAL TECHNIQUES FOR STRUCTURAL ANALYSIS OF GLYCOSAMINOGLYCANS

糖胺聚糖结构分析的分析技术

• 批准号: 8363035
• 负责人: Parastoo Azadi
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363035
• 关键词: Biochemical; Detection; Disaccharides; Educational process of instructing; Enzymes; Funding; GAG Gene; Glycosaminoglycans; Grant; Ion-Exchange Chromatography Procedure; Learning; Mass Spectrum Analysis; National Center for Research Resources; Nitrous Acid; Oligosaccharides; Participant; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Techniques; Technology; Time; United States National Institutes of Health; cost; depolymerization; experience; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This was a new course, which was taught for the first time August 25-27, 2008. Participants learned basic techniques for the structural characterization of glycosaminoglycans (GAGs), which included depolymerization of the GAG polysaccharides to its disaccharide constituents using appropriate enzymes. The disaccharides were separated by ion exchange chromatography and the products detected by U.V. The disaccharides were derivatized with 2-cyanoacetamide and were detected with fluorescent detection. In a separate experiment, the glycosaminoglycans were hydrolyzed by nitrous acid and the products analyzed. Mass spectrometry techniques for analysis of di- and oligosaccharides derived from glycosaminoglycans were also covered. Experience with basic biochemical techniques is a prerequisite for participation.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这是一门新课程，2008年8月25日至27日首次授课。与会者学习了糖胺多聚糖(GAG)结构表征的基本技术，包括使用适当的酶将GAG多糖解聚为其二糖组分。二糖用离子交换层析分离，产物用紫外可见分光光度法检测，用2-氰基乙酰胺衍生化，荧光检测。在另一项实验中，用亚硝酸对糖胺多聚糖进行水解，并对产物进行分析。还介绍了从糖胺多聚糖中提取的二糖和低聚糖的质谱学分析技术。基本的生化技术经验是参加的先决条件。