COMPUTATIONAL MODELING OF THE STRUCTURE OF THE SPINDLE POLE BODY CORE

主轴极体铁芯结构的计算模型

• 批准号: 8365787
• 负责人: ANDREJ SALI
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365787
• 关键词: Architecture; Biology; Cell Death; Cell division; Cells; Centrosome; Chromosome Segregation; Chromosomes; Complex; Computer Simulation; Computer software; Data; Development; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Genetic Materials; Grant; Modeling; Multiprotein Complexes; National Center for Research Resources; Principal Investigator; Process; Regulation; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; Yeasts; cost; crosslink; insight; protein structure; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, developmental errors, and cell death. Crucial to proper partitioning of the chromosomes during cell division is the accurate duplication, assembly and regulation of the centrosome, which is responsible for forming the complex protein structure that segregates the genetic material. The spindle pole body (SPB) in yeast is the best characterized centrosome. Understanding its structure and assembly will provide important insights into the essential process of distributing the chromosomes in each cell division. The Sali lab developed the IMP software package to construct detailed models of the architecture of large macromolecular protein complexes. We propose to use IMP in an iterative process to construct a high resolution model of the central core of the yeast SPB. The distance constraints for the construction of the model will rely on currently available data and data generated by FRET and cross-linking analysis.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 所有细胞的传播都需要准确的染色体分离。此过程中的错误与肿瘤发生，发育错误和细胞死亡有关。对于细胞分裂过程中染色体的正确分配至关重要的是中心体的准确复制，组装和调节，该复制和调节负责形成将遗传物质分离的复杂蛋白质结构。酵母中的主轴杆体（SPB）是特征最佳的中心体。了解其结构和组装将为每个细胞分裂中分布染色体的基本过程提供重要的见解。 Sali Lab开发了IMP软件包，以构建大型大分子蛋白质复合物的结构的详细模型。我们建议在迭代过程中使用IMP来构建酵母SPB中心核心的高分辨率模型。模型构建的距离约束将依赖于当前可用的数据和FRET和交联分析生成的数据。