COMPUTATIONAL MODELING OF THE STRUCTURE OF THE SPINDLE POLE BODY CORE

主轴极体铁芯结构的计算模型

• 批准号: 8365787
• 负责人: ANDREJ SALI
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365787
• 关键词: Architecture; Biology; Cell Death; Cell division; Cells; Centrosome; Chromosome Segregation; Chromosomes; Complex; Computer Simulation; Computer software; Data; Development; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Genetic Materials; Grant; Modeling; Multiprotein Complexes; National Center for Research Resources; Principal Investigator; Process; Regulation; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; Yeasts; cost; crosslink; insight; protein structure; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, developmental errors, and cell death. Crucial to proper partitioning of the chromosomes during cell division is the accurate duplication, assembly and regulation of the centrosome, which is responsible for forming the complex protein structure that segregates the genetic material. The spindle pole body (SPB) in yeast is the best characterized centrosome. Understanding its structure and assembly will provide important insights into the essential process of distributing the chromosomes in each cell division. The Sali lab developed the IMP software package to construct detailed models of the architecture of large macromolecular protein complexes. We propose to use IMP in an iterative process to construct a high resolution model of the central core of the yeast SPB. The distance constraints for the construction of the model will rely on currently available data and data generated by FRET and cross-linking analysis.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 所有细胞的繁殖都需要准确的染色体分离。这一过程中的错误与肿瘤发生、发育错误和细胞死亡有关。细胞分裂过程中染色体正确分配的关键是中心体的精确复制、组装和调节，中心体负责形成分离遗传物质的复杂蛋白质结构。酵母中的纺锤体极体（SPB）是最具特征的中心体。了解其结构和组装将为了解每次细胞分裂中染色体分布的基本过程提供重要的见解。 Sali 实验室开发了 IMP 软件包来构建大分子蛋白质复合物结构的详细模型。我们建议在迭代过程中使用 IMP 来构建酵母 SPB 中央核心的高分辨率模型。模型构建的距离约束将依赖于当前可用的数据以及 FRET 和交联分析生成的数据。