COMPUTATIONAL MODELING OF THE STRUCTURE OF THE SPINDLE POLE BODY CORE

主轴极体铁芯结构的计算模型

• 批准号: 8365787
• 负责人: ANDREJ SALI
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365787
• 关键词: Architecture; Biology; Cell Death; Cell division; Cells; Centrosome; Chromosome Segregation; Chromosomes; Complex; Computer Simulation; Computer software; Data; Development; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Genetic Materials; Grant; Modeling; Multiprotein Complexes; National Center for Research Resources; Principal Investigator; Process; Regulation; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; Yeasts; cost; crosslink; insight; protein structure; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, developmental errors, and cell death. Crucial to proper partitioning of the chromosomes during cell division is the accurate duplication, assembly and regulation of the centrosome, which is responsible for forming the complex protein structure that segregates the genetic material. The spindle pole body (SPB) in yeast is the best characterized centrosome. Understanding its structure and assembly will provide important insights into the essential process of distributing the chromosomes in each cell division. The Sali lab developed the IMP software package to construct detailed models of the architecture of large macromolecular protein complexes. We propose to use IMP in an iterative process to construct a high resolution model of the central core of the yeast SPB. The distance constraints for the construction of the model will rely on currently available data and data generated by FRET and cross-linking analysis.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 所有细胞的繁殖都需要精确的染色体分离。这个过程中的错误与肿瘤发生、发育错误和细胞死亡有关。在细胞分裂过程中，染色体正确分配的关键是中心体的准确复制、组装和调节，中心体负责形成分离遗传物质的复杂蛋白质结构。纺锤体是酵母中最具特征的中心体。了解它的结构和组装将提供重要的见解，在每个细胞分裂中分配染色体的基本过程。 Sali实验室开发了IMP软件包，用于构建大分子蛋白质复合物结构的详细模型。我们建议使用IMP在一个迭代的过程中构建一个高分辨率的模型的中央核心的酵母SPB。用于构建模型的距离约束将依赖于当前可用的数据以及通过FRET和交联分析生成的数据。