COMPUTATIONAL MODELING OF THE STRUCTURE OF THE SPINDLE POLE BODY CORE

主轴极体铁芯结构的计算模型

• 批准号: 8365787
• 负责人: ANDREJ SALI
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365787
• 关键词: Architecture; Biology; Cell Death; Cell division; Cells; Centrosome; Chromosome Segregation; Chromosomes; Complex; Computer Simulation; Computer software; Data; Development; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Genetic Materials; Grant; Modeling; Multiprotein Complexes; National Center for Research Resources; Principal Investigator; Process; Regulation; Research; Research Infrastructure; Resolution; Resources; Source; Structure; United States National Institutes of Health; Yeasts; cost; crosslink; insight; protein structure; spindle pole body; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, developmental errors, and cell death. Crucial to proper partitioning of the chromosomes during cell division is the accurate duplication, assembly and regulation of the centrosome, which is responsible for forming the complex protein structure that segregates the genetic material. The spindle pole body (SPB) in yeast is the best characterized centrosome. Understanding its structure and assembly will provide important insights into the essential process of distributing the chromosomes in each cell division. The Sali lab developed the IMP software package to construct detailed models of the architecture of large macromolecular protein complexes. We propose to use IMP in an iterative process to construct a high resolution model of the central core of the yeast SPB. The distance constraints for the construction of the model will rely on currently available data and data generated by FRET and cross-linking analysis.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 所有细胞的繁殖都需要准确的染色体分离。这一过程中的错误与肿瘤发生、发育错误和细胞死亡有关。在细胞分裂过程中，染色体正确划分的关键是中心体的准确复制、组装和调节，中心体负责形成分离遗传物质的复杂蛋白质结构。酵母中的纺锤体极体(SPB)是最具特征的中心体。了解它的结构和组装将为在每一次细胞分裂中分配染色体的基本过程提供重要的见解。 SARI实验室开发了IMP软件包，以构建大分子蛋白质复合体的详细结构模型。我们建议在迭代过程中使用IMP来构建酵母SPB中心核心的高分辨率模型。构建模型的距离限制将取决于目前可用的数据和通过FRET和交叉链接分析产生的数据。