Research on inhalation toxicology of environmental chemicals

2011财年期间，为支持NTP研究的4名首席调查员进行了大约28项研究，为支持DIR研究(LRB)的5名首席调查员进行了大约89项研究。这些研究是为了支持对大约13名博士后、研究生和其他非PI调查人员的研究。在2011财年期间，1873只动物(约20只小鼠品系和3只大鼠品系)被接收并保存在吸入设施进行这些研究。这些动物中的大多数是用合同资金购买的。从主校园动物设施不接受的来源(杜克大学、NICHD、北卡罗来纳大学)收到了一些转基因小鼠品系。此外，还建立了4个未经批准来源的转基因小鼠品系的动物繁育群体。大约13种不同的化学制剂(卵清蛋白、臭氧、高氧、2，3-丁二酮、丙酮、2，3-戊二酮、苯、磷化铟、氧化铟、苯霉素A1、高碳酸气、乙酰甲胆碱、香烟烟雾)和大约7种生物化合物(脂多糖、癌症干细胞、树突状细胞、白喉毒素、透明质酸结合蛋白、脂质体、屋尘提取物)处理动物。采用全身吸入、鼻腔吸入、气管内滴注、口咽抽吸、腹腔注射、胸腔注射等方法给药。除了动物的维护和治疗，技术人员还进行了尸检，收集了支气管肺泡液和组织用于组织病理学或分子分析，进行了蛋白质和乳酸脱氢酶分析，并使用Flexivent系统评估了肺功能。

During FY2011 approximately 28 studies were conducted for 4 principal investigators in support of NTP research, and approximately 89 studies were conducted for 5 principal investigators in support of DIR research (LRB). These studies were conducted in support of research for approximately 13 post-docs, graduate students and other non-PI investigators. During FY2011, 1873 animals (~20 mouse strains and 3 rat strains) were received and maintained at the Inhalation facility for these studies. Most of these animals were purchased using contract funds. A number of genetically-modified mouse strains were received from sources (Duke, NICHD, UNC) that are not accepted at the main campus animal facility. In addition animal breeding colonies were set up for 4 strains of genetically-modified mice from unapproved sources. Animals were treated with approximately 13 different chemical agents (ovalbumin, , ozone, hyperoxia, 2,3-butanedione, acetoin, 2,3-pentanedione, benzene, indium phosphide, indium-tin-oxide, bafilomycin A1, hypercapnic gases, methacholine, cigarette smoke), and approximately 7 biological compounds (lipopolysaccharide, cancer stem cells, dendritic cells, diphtheria toxin, hyaluronan binding protein, liposomes, house dust extract). Chemicals and biological agents were administered by whole-body inhalation, nose-only inhalation, intratracheal instillation, oropharyngeal aspiration, intraperitoneal injection, and intrapleural injection. In addition to animal maintenance and treatment, technicians also performed necropsies, collected bronchoalveolar fluid, and tissues for histopathology or molecular analysis, conducted protein and LDH analyses and evaluated pulmonary function using the Flexivent system.