Macromolecular Crystallography at the National Synchrotron Light Source

位于国家同步加速器光源（NSLS）的大分子晶体学研究资源（PXRR）运行着六条光束线，这些光束线被大约160个外部研究小组和六个布鲁克海文实验室小组用于研究。这些科学家通常在一年中发表200多篇研究论文，去年在蛋白质数据库中发表了300多篇论文。PXRR开创了一个积极的Mail-in项目，包括与大约15个外部研究小组的积极合作，以及与更多小组的短期合作或服务项目。在过去的几年里，这个项目已经与90个小组进行了互动，在过去的四年里，这个项目每年都有大约25个PDB的沉积和15个出版物。

The Macromolecular Crystallography Research Resource (PXRR) at the National Synchrotron Light Source (NSLS) operates six beamlines that are employed for research by about 160 outside research groups and half a dozen Brookhaven Lab groups. These scientists produce over 200 research publications in a typical year, and over 300 Protein Data Bank depositions in the last year. The PXRR have pioneered an active Mail-in program that involves active collaborations with about 15 outside research groups, and short collaborations or service projects with many more. This program has interacted with 90 groups in the last several years, and there have been about 25 PDB depositions and 15 publications from this program in each of the last four years. The PXRR performs research and development on apparatus, methods, and software to improve the technology for synchrotron-based macromolecular crystallography. Special emphasis during the coming cycle will be on development of detectors that will match the power and collimation of modern x-ray sources, use of micron-sized crystals, development of robotic cryogenic specimen handling, and simultaneous measurement of UV, Visible, or Raman IR spectra with x-ray diffraction. The PXRR staff will execute numerous small research programs, among other things to improve visualization of tiny specimens and improve cryogenic preservation of specimens. A long-term goal for the PXRR is to create a resource for macromolecular crystallography at NSLS-II, the new synchrotron light source that is being created at Brookhaven Lab during the next five to seven years. The objective will be to have several macromolecular crystallography beamlines in operation when NSLS-II begins operation. This project will involve direct cooperation with NSLS-II staff to develop a complete plan for such facilities, and then to begin to achieve that plan.