Gastric Histopathology Core

胃组织学核心的主要使命是提供高质量，快速周转 通过与Kay华盛顿博士和M.布兰卡·皮亚苏埃洛建议提供下列服务： 1.目的：为啮齿类动物H.幽门螺杆菌致胃肿瘤及其与人类疾病的关系 2.提供开发、执行和评估免疫组织化学研究的专业知识， 动物组织，具有稳健的质量控制措施，并在需要时扩展至人体组织 3.提供激光显微切割服务 4.提供组织微阵列服务，并根据需要获取人类胃组织样本 该计划项目的三个组成项目和核心B（蛋白质组学核心）都严重依赖于从幽门螺杆菌诱导的胃炎和胃肿瘤的啮齿动物模型中分离的胃组织的形态学分析。华盛顿博士和Piazuelo博士在啮齿动物模型组织病理学变化解释和免疫组织化学研究解释方面的专业知识对这项工作至关重要，这些合作活动目前没有通过现有资源提供资金。通过利用GI研究相关的免疫组织化学设施的资源，将有可能实现本补助金中提出的所有组织学研究的高标准。在对胃肠道病理学有专门兴趣的经验丰富的病理学家的监督下，这种组织学服务的集中化将允许对质量保证的仔细关注。

The primary mission of the Gastric Histopathology Core is to provide high quality, rapid-turnaround immunohistochemistry and specialized research histology services to investigators in this PPG, and to provide expert histopathologic interpretation, through collaboration with Dr. Kay Washington and Dr. M. Blanca Piazuelo. The following services are proposed: 1. To provide expert evaluation of histopathology of rodent models of H. pylori-induced gastric neoplasia and correlation with human disease 2. To provide expertise in developing, performing and evaluating immunohistochemical studies on animal tissues, with robust quality control measures, and with extension to human tissue if needed 3. To provide laser microdissection services 4. To provide tissue microarray services and access to human gastric tissue samples as needed The three component projects and Core B (Proteomics Core) of this proposed Program Project all rely heavily upon morphologic analysis of gastric tissues isolated from rodent models of Helicobacter pylori-induced gastritis and gastric neoplasia. Dr. Washington's and Dr. Piazuelo's expertise in interpretation of histopathologic changes in rodent models and in interpretation of immunohistochemical studies will be critical to this work, and these collaborative activities are currently not funded through existing resources. By utilizing the resources of an Gl research-related immunohistochemistry facility, it will be possible to achieve high standards for all histologic studies proposed in this grant. This centralization of histologic services under the supervision of experienced pathologists with a dedicated interest in gastrointestinal pathology will allow careful attention to quality assurance.