Core 1: Tissue Pathology and Cellular Analysis

核心 1：组织病理学和细胞分析

• 批准号: 10700840
• 负责人: MARY Kay WASHINGTON
• 金额: $ 31.13万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-09 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10700840
• 关键词: Academic Medical Centers; Animal Cancer Model; Antibodies; Basic Science; Behavior; Bioinformatics; Biological Assay; Biological Process; Biometry; Cancer Center; Cancer Science; Cells; Collaborations; Collection; Colorectal Cancer; Colorectal Neoplasms; Computer Analysis; Confidentiality of Patient Information; Consult; Consultations; Cooperative Human Tissue Network; Custom; Cytometry; Data; Data Analyses; Databases; Diagnosis; Digestive System Disorders; Disease; Early Diagnosis; Ensure; Epithelial Cells; Epithelium; Evaluation; Formalin; Freezing; Gastrointestinal Neoplasms; Gene Expression; Generations; Genetic Complementation Test; Histologic; Histology; Histopathology; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; Informatics; Informed Consent; Infrastructure; Malignant neoplasm of gastrointestinal tract; Mission; Molecular; Morphology; Mus; Normal tissue morphology; Paraffin Embedding; Pathology; Patient Care; Patients; Play; Pre-Clinical Model; Preparation; Prevention; Process; Publishing; Qualifying; Quality Control; Reagent; Reproduction spores; Research; Research Activity; Research Personnel; Resource Sharing; Role; Running; Sampling; Services; Signal Transduction; Specimen; Technology; The Vanderbilt-Ingram Cancer Center at the Vanderbilt University; Time; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Translational Research; Validation; Washington; Work; biobank; cancer biomarkers; cellular imaging; colorectal cancer treatment; cost effective; data de-identification; data quality; digital; flexibility; human disease; image processing; instrument; laser capture microdissection; molecular imaging; mouse model; next generation; novel; operation; protein expression; repository; screening; single cell analysis; single-cell RNA sequencing; synergism; tool; translational goal

Project Summary/Abstract: Tissue Pathology and Cellular Analysis Core The Translational Pathology and Cellular Analysis (TPCA) Core, will play a central role in the translational research mission of this SPORE, providing essential support for all projects by providing annotated human biospecimens and research histology services. It includes with a single-cell analysis subcore under the direction of Dr. Ken Lau. This subcore will enable technologies for quantifying and analyzing protein expression at a multiplex, single-cell level, greatly augmenting the histopathology capabilities of the TPCA on both FFPE, fresh, and frozen tissue samples. While the primary mission of the TPCA is state-of-the-art immunohistochemistry, research pathology and biorepository services, the single-cell analysis subcore will offer three cutting-edge technologies: inDrop single-cell RNA-Seq, MxIF, and DISSECT-CyTOF, and their associated computational analyses. The single-cell analysis subcore will offer services, from the preparation and processing of samples, the generation and verification of epithelial-specific reagents, screening and validation of targets, and finally, computational data analysis. Single-cell data will be synthesized and presented to investigators in adaptable formats for presentation and/or downstream analysis. A specific strength of this subcore in relation to the GI SPORE is that Dr. Ken Lau's research focus is on GI disorders and his expertise will greatly synergize with the research of GI neoplasms. We can offer highly specific molecular assays that can flexibly query a wide range of biological processes including the evaluation of signaling, cell identity, function, and behavior, all within a single cell. The TPCA will partner with other established tissue collection and immunohistochemistry shared resources, such as the Vanderbilt University Medical Center Translational Pathology Shared Resource and the Digital Histology Shared Resource, to provide exceptional services to the GI SPORE in a highly cost-effective manner. Close collaboration with the Biostatistics and Bioinformatics Core will provide the TPCA and GI SPORE with robust informatics and database tools for collection, distribution, and annotation of biospecimens in the GI SPORE repository. Dr. Washington and Dr. Lau have developed synergistic working relationships with imaging services under the direction of Dr. Charles Manning and these will be maintained between the TPCA and the Mouse and Human Molecular Imaging Core.

项目摘要/摘要：组织病理学和细胞分析核心 翻译病理学和细胞分析（TPCA）核心，将在翻译过程中发挥核心作用。 该SPORE的研究使命，通过提供注释的人类信息，为所有项目提供必要的支持。 生物标本和研究组织学服务。它包括一个单细胞分析子核心的指导下， Ken Lau医生该子核心将使定量和分析蛋白质表达的技术在一个特定的时间点上成为可能。 多重，单细胞水平，大大增强了TPCA对FFPE，新鲜， 和冷冻组织样本虽然TPCA的主要使命是最先进的免疫组织化学， 研究病理学和生物储存服务，单细胞分析子核心将提供三个尖端 技术：inDrop单细胞RNA-Seq、MxIF和DISSECT-CyTOF及其相关的计算 分析。单细胞分析子核心将提供服务，从样品的制备和处理， 上皮特异性试剂的产生和验证，靶的筛选和验证，以及最后， 计算数据分析单细胞数据将被合成并以可适应的方式呈现给研究人员。 用于呈现和/或下游分析的格式。该次核心相对于GI的具体强度 特别是刘健博士的研究重点是胃肠道疾病，他的专业知识将大大协同 GI肿瘤的研究。我们可以提供高度特异性的分子检测，可以灵活地查询广泛的 生物学过程，包括信号传导、细胞身份、功能和行为的评估，所有这些都在一个单一的 cell. TPCA将与其他已建立的组织收集和免疫组织化学共享资源合作， 如范德比尔特大学医学中心转化病理学共享资源和数字 组织学共享资源，以高成本效益的方式为GI SPORE提供卓越的服务。 与生物统计学和生物信息学核心的密切合作将为TPCA和GI SPORE提供 强大的信息学和数据库工具，用于收集、分发和注释GI中的生物标本 SPORE存储库。华盛顿博士和刘博士在影像学方面建立了协同工作关系 在查尔斯·曼宁博士的指导下提供服务，这些服务将在TPCA和 小鼠和人类分子成像核心。