Tissue and Cell Processing Core

组织和细胞处理核心的主要目标是向项目调查人员提供：(1) 石蜡包埋、冷冻和塑料包埋组织标本的最高质量处理 (2)组织和细胞标本的高保真原代培养；(3)高通量染色以 促进基于体视学的组织定量组织形态计量学(用于组织)和免疫组织化学 染色(用于组织和细胞培养)。组织和细胞处理核心是 这份AADCRC申请，有助于实现三项计划提案的目标，其中每一项 依靠对人和鼠组织的各种组织学分析以及对人和鼠的体外研究 鼻腔、气管和支气管上皮细胞。核心技术人员的丰富经验和 领导力促进优化协议的开发，以实现每个项目的目标和 还提供一致的高质量实验材料，这些材料通常在项目之间共享。这个 核心的专业知识对于优化从人类和特定小鼠组织样本中提取的产量至关重要 很难获得，而且令人望而却步。这个核心最初是在几年前开发的，已经 始终如一地为100多名项目调查人员及其合作者提供最高质量的服务 在华盛顿大学和世界各地。信元处理核心的添加提供了 有能力获得高产量、仔细表型的原代上皮细胞的肺部研究人员 人和小鼠肺标本的培养在气液界面上分化，用于 机械性的体外研究。CORE提供另外两项大型中心资助(慢性阻塞性肺病的SCCOR和PPG 在肺重塑中)实现了相当大的成本节约和人员高效利用 具备在高吞吐量环境中的专业技能。

The major objectives of the Tissue and Cell Processing Core are to provide to Program Investigators: (1) the highest quality processing of tissue specimens for paraffin-embedded, frozen- and plastic-embedded sections; (2) high fidelity primary cultures from tissue and cell specimens; (3) high throughput staining to facilitate stereology-based tissue quantitative histomorphometry (for tissues) and immunohistochemical staining (for tissues and cell cultures). The Tissue and Cell Processing Core is an essential component of this AADCRC application, facilitating carrying out the aims of the three Program proposals, each of which rely on varied histologic analyses of human and mouse tissues and in vitro studies of human and mouse nasal, tracheal and bronchial epithelial cells. The considerable experience of Core technical personnel and leadership facilitates the development of optimized protocols to achieve the objectives of each Project and also provides consistent high quality experimental materials which are often shared across Projects. The expertise of the Core is essential to optimizing yield from human and specific mouse tissue samples which are difficult to obtain and prohibitive to replace. This Core was initially developed over IS years ago and has consistently provided the highest quality services to over 100 Program Investigators and their collaborators at Washington University and around the world. The addition of the Cell Processing Core provides pulmonary investigators with the ability to acquire high yield, carefully phenotyped primary epithelial cell cultures from human and mouse lung specimens differentiated at the air-liquid interface, used for mechanistic in vitro studies. The Core serves two other large center grants (a SCCOR in COPD and a PPG in Lung Remodeling) enabling considerable cost savings and efficient utilization of personnel with specialized skills in a high throughput environment.