Tissue and Cell Processing Core

组织和细胞处理核心

• 批准号: 8701221
• 负责人: Richard A Pierce
• 金额: $ 18.7万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8701221
• 关键词: Air; Allergic Disease; Asthma; Atopic Dermatitis; Autophagocytosis; Cell Culture Techniques; Cell Separation; Cell physiology; Cells; Chronic Obstructive Airway Disease; Cost Savings; Development; Ectopic Expression; Environment; Epithelial; Epithelial Cells; Epithelium; Experimental Models; Flow Cytometry; Freezing; Gene Silencing; Gene Targeting; Genetic; Goals; Grant; Histologic; Human; Human Resources; Immunohistochemistry; In Vitro; Individual; Interferons; Leadership; Liquid substance; Lung; Methods; Mus; Nose; Paraffin Embedding; Pathogenesis; Pathway interactions; Phenotype; Plastic Embedding; Process; Productivity; Protocols documentation; Research Personnel; Resources; Role; Sampling; Services; Signal Pathway; Signal Transduction; Slide; Specimen; Staining method; Stains; Structure of parenchyma of lung; Suspension substance; Suspensions; Techniques; Testing; Tissue Fixation; Tissue Model; Tissue Sample; Tissues; Universities; Virus Diseases; Washington; atopy; base; cost; cost efficient; digital imaging; experience; human tissue; immunocytochemistry; meetings; mouse model; programs; respiratory; skills; tissue processing; tissue resource; tissue/cell culture

The major objectives of the Tissue and Cell Processing Core are to provide to Program Investigators: (1) the highest quality processing of tissue specimens for paraffin-embedded, frozen- and plastic-embedded sections; (2) high fidelity primary cultures from tissue and cell specimens; (3) high throughput staining to facilitate stereology-based tissue quantitative histomorphometry (for tissues) and immunohistochemical staining (for tissues and cell cultures). The Tissue and Cell Processing Core is an essential component of this AADCRC application, facilitating carrying out the aims of the three Program proposals, each of which rely on varied histologic analyses of human and mouse tissues and in vitro studies of human and mouse nasal, tracheal and bronchial epithelial cells. The considerable experience of Core technical personnel and leadership facilitates the development of optimized protocols to achieve the objectives of each Project and also provides consistent high quality experimental materials which are often shared across Projects. The expertise of the Core is essential to optimizing yield from human and specific mouse tissue samples which are difficult to obtain and prohibitive to replace. This Core was initially developed over IS years ago and has consistently provided the highest quality services to over 100 Program Investigators and their collaborators at Washington University and around the world. The addition of the Cell Processing Core provides pulmonary investigators with the ability to acquire high yield, carefully phenotyped primary epithelial cell cultures from human and mouse lung specimens differentiated at the air-liquid interface, used for mechanistic in vitro studies. The Core serves two other large center grants (a SCCOR in COPD and a PPG in Lung Remodeling) enabling considerable cost savings and efficient utilization of personnel with specialized skills in a high throughput environment.

组织和细胞处理核心的主要目标是为项目研究者提供：（1） 最高质量的石蜡包埋、冷冻和塑料包埋组织标本处理 切片;（2）来自组织和细胞标本的高保真原代培养物;（3）高通量染色， 促进基于体视学的组织定量组织形态测定（用于组织）和免疫组织化学 染色（用于组织和细胞培养）。组织和细胞处理核心是 这一AADCRC应用程序，促进实现三个计划提案的目标，每个提案 依赖于对人类和小鼠组织的各种组织学分析以及对人类和小鼠的体外研究 鼻、气管和支气管上皮细胞。核心技术人员的丰富经验， 领导促进优化协议的开发，以实现每个项目的目标， 还提供一致的高质量实验材料，这些材料通常在项目之间共享。的 核心的专业知识对于优化人类和特定小鼠组织样本的产量至关重要， 很难获得并且不允许更换。这个核心最初是在几年前开发的， 始终如一地为100多名项目研究人员及其合作者提供最高质量的服务 在华盛顿大学和世界各地。细胞处理核心的加入提供了 肺研究者能够获得高产量、仔细分型的原代上皮细胞 来自人和小鼠肺标本的培养物在空气-液体界面处分化，用于 体外机制研究。核心基金还提供另外两个大型中心赠款（COPD SCCOR和PPG 在肺重塑中）能够节省大量成本并有效利用人员， 在高吞吐量环境中的专业技能。