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Tissue and Cell Processing Core

组织和细胞处理核心

基本信息

批准号：
8889186
负责人：
Richard A Pierce
金额：
$ 14.52万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8889186
关键词：
Air Allergic Disease Asthma Atopic Dermatitis Autophagocytosis Cell Culture Techniques Cell Separation Cell physiology Cells Chronic Obstructive Airway Disease Cost Savings Development Ectopic Expression Environment Epithelial Epithelial Cells Epithelium Experimental Models Flow Cytometry Freezing Gene Silencing Gene Targeting Genetic Goals Grant Histologic Human Human Resources Immunohistochemistry In Vitro Individual Interferons Leadership Liquid substance Lung Methods Mus Nose Paraffin Embedding Pathogenesis Pathway interactions Phenotype Plastic Embedding Process Productivity Protocols documentation Research Personnel Resources Role Sampling Services Signal Pathway Signal Transduction Slide Specimen Staining method Stains Structure of parenchyma of lung Suspension substance Suspensions Techniques Testing Tissue Fixation Tissue Model Tissue Sample Tissues Universities Virus Diseases Washington asthmatic atopy base cost cost efficient digital imaging experience human tissue immunocytochemistry meetings mouse model programs respiratory skills tissue processing tissue resource tissue/cell culture

项目摘要

The major objectives of the Tissue and Cell Processing Core are to provide to Program Investigators: (1) the highest quality processing of tissue specimens for paraffin-embedded, frozen- and plastic-embedded sections; (2) high fidelity primary cultures from tissue and cell specimens; (3) high throughput staining to facilitate stereology-based tissue quantitative histomorphometry (for tissues) and immunohistochemical staining (for tissues and cell cultures). The Tissue and Cell Processing Core is an essential component of this AADCRC application, facilitating carrying out the aims of the three Program proposals, each of which rely on varied histologic analyses of human and mouse tissues and in vitro studies of human and mouse nasal, tracheal and bronchial epithelial cells. The considerable experience of Core technical personnel and leadership facilitates the development of optimized protocols to achieve the objectives of each Project and also provides consistent high quality experimental materials which are often shared across Projects. The expertise of the Core is essential to optimizing yield from human and specific mouse tissue samples which are difficult to obtain and prohibitive to replace. This Core was initially developed over IS years ago and has consistently provided the highest quality services to over 100 Program Investigators and their collaborators at Washington University and around the world. The addition of the Cell Processing Core provides pulmonary investigators with the ability to acquire high yield, carefully phenotyped primary epithelial cell cultures from human and mouse lung specimens differentiated at the air-liquid interface, used for mechanistic in vitro studies. The Core serves two other large center grants (a SCCOR in COPD and a PPG in Lung Remodeling) enabling considerable cost savings and efficient utilization of personnel with specialized skills in a high throughput environment.

组织和细胞处理核心的主要目标是为项目调查员提供：(1)