Molecular Core

分子核心

• 批准号: 8739550
• 负责人: Savio Chan
• 金额: $ 9.87万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8739550
• 关键词: Basal Ganglia; Cells; Consultations; Core Facility; Fluorescence-Activated Cell Sorting; Gene Delivery; Gene Expression; Gene Expression Profiling; Genes; Genotype; Goals; Human Resources; Methods; Molecular; Neurons; Oxidation-Reduction; Periodicity; Pharmacogenomics; Population; Reporting; Research Personnel; Schedule; Services; Supervision; Technology; Time; Tissues; Transgenic Animals; Viral Vector; Workload; base; design and construction; experience; genetic manipulation; in vivo; meetings; operation; optogenetics; programs; research study; success

Our Udall Center proposal continues to have a Molecular Core intended to provide scientific support to the re- search teams. The Molecular Core will provide four services to the program team. The first is to provide gene expression analysis using a combination of fluorescence activated cell sorting (FACS) and quantitative real time PCR (qPCR). Projects 1, 2, 3, and 5 make use of this approach. The second service is to provide consultation and assistance for genotyping. The projects 1, 2, 3 and 4 makes extensive use of transgenic animals that require accurate and timely genotyping. The third service is to provide assistance in the design and construction of viral vectors for gene knockdown. Both projects 2 and 3 make extensive use of this technology. The third service is to provide assistance in the design and construction of viral vectors for gene delivery. Projects 1, 2, 3 and 5 make use of this service for delivery of various expression constructs related to optogenetics, pharmacogenomics and redox reporting. Dr. C. Savio Chan, will serve as the Core Director and oversee the operation of the core. Dr. Chan has extensive experience with gene expression studies, including: single-cell, FACS-based cell-population, and tissue-level analyses. All necessary methods in the Molecular Core facility have been established previously. The Molecular Core will be administered by Dr. Chan with the assistance of Dr. Jyothisri Kondapalli. Together, they will be responsible for the day-to-day operation of the core, supervision of technical staff, purchasing supplies, etc.

我们的Udall中心建议继续有一个分子核心，旨在为重新提供科学支持， 搜索队分子核心将为项目团队提供四项服务。第一，提供基因 使用荧光激活细胞分选（FACS）和定量真实的时间的组合的表达分析 PCR（qPCR）。项目1、2、3和5使用这种方法。第二项服务是提供咨询 并协助基因分型。项目1、2、3和4广泛使用转基因动物， 准确和及时的基因分型。第三项服务是提供协助设计和建设病毒 用于基因敲除的载体。项目2和项目3都广泛使用了这项技术。第三项服务是 在设计和构建用于基因递送的病毒载体方面提供帮助。项目1、2、3和5使 使用该服务递送与光遗传学、药物基因组学和免疫学相关的各种表达构建体， 氧化还原报告。C医生Savio Chan先生将担任核心董事并监督核心的运作。陈医生 在基因表达研究方面拥有丰富的经验，包括：单细胞、基于FACS的细胞群，以及 组织水平分析。分子核心设施中的所有必要方法先前已确立。的 分子核心将由Chan博士在Jyothisri Kondapalli博士的协助下进行。他们一起 负责核心的日常运作，监督技术人员，采购物资等。