CSHL Course on Advanced Sequencing Technologies & Applications

描述(由申请者提供)：冷泉港高级测序技术与应用实验室课程将于2011年11月8日至21日、2012年和2013年举行。在过去的十年里，大规模的DNA测序已经显著地影响了现代生物学的实践，并开始影响医学实践。随着最近几种先进测序技术的引入，成本和时间线已经减少了几个数量级，便于研究人员概念化和执行迄今为止令人望而却步的基于测序的项目。此外，应用这些技术来回答以前无法通过实验接近的问题，正在扩大它们的影响和应用。冷泉港实验室建议继续提供为期两周的高级课程，名为高级测序技术和应用，每年秋季在该实验室新建的和专门建造的教学设施中提供。预计将有多种科学背景的应用，包括分子进化、发育、神经科学、癌症、植物生物学和微生物学。将指导学生详细操作几个革命性的测序平台，包括样品准备程序、通过管道处理一般数据以及深入数据分析。将探索一系列不同的生物学问题，包括人类基因组区域的DNA重新测序，细菌基因组的从头DNA测序，以及这些技术在研究小RNA中的使用等。客座讲师为课程做出贡献，并鼓励他们强调自己对这些革命性技术的应用。这个冷泉港年度短期课程在住宿环境中提供专门的培训，这会立即影响学员的研究进度。该课程还促进了教师和受训人员之间的合作和思想交流，因此通过教育精选的研究人员干部这些学科中的最新方法和概念，对加快基因组研究来说是一项重要而有价值的投资。该课程在培训那些在科学领域取得成功的研究人员方面有着良好的记录。

DESCRIPTION (provided by applicant): The proposed Cold Spring Harbor Laboratory course on Advanced Sequencing Technologies & Applications is to be held November 8-21, 2011, 2012 and 2013. Over the last decade, large scale DNA sequencing has markedly impacted the practice of modern biology and is beginning to affect the practice of medicine. With the recent introduction of several advanced sequencing technologies, costs and timelines have been reduced by orders of magnitude, facilitating investigators to conceptualize and perform sequencing-based projects that heretofore were prohibitive. Furthermore, the application of these technologies to answer questions previously not experimentally approachable is broadening their impact and application. Cold Spring Harbor Laboratory is proposing to continue offering an advanced two-week course entitled Advanced Sequencing Technologies & Applications, to be offered annually each fall in newly constructed and purpose-built teaching facilities at the Laboratory. Applications are anticipated from a diversity of scientific backgrounds including molecular evolution, development, neuroscience, cancer, plant biology and microbiology. Students will be instructed in the detailed operation of several revolutionary sequencing platforms, including sample preparation procedures, general data handling through pipelines, and in-depth data analysis. A diverse range of biological questions will be explored including DNA re-sequencing of human genomic regions, de novo DNA sequencing of bacterial genomes, and the use of these technologies in studying small RNAs, among others. Guest lecturers contribute to the course and are encouraged to highlight their own applications of these revolutionary technologies. This Cold Spring Harbor annual short course offers specialized training in a residential setting that immediately impacts the progress of the trainees' research. The course also promotes collaboration and exchange of ideas between faculty and trainees, and thus represents a significant and valuable investment towards accelerating genome research by educating select cadres of researchers in the latest methods and concepts in these disciplines. The course has a proven track record of training investigators who go on to successful careers in science.