National Exposure Assessment Laboratory at Emory

埃默里国家暴露评估实验室

• 批准号: 9062183
• 负责人: GARY W MILLER
• 金额: $ 106.13万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9062183
• 关键词: Behavior; Binding; Biological; Biological Assay; Biological Markers; Biology; Chemical Exposure; Chemicals; Client; Coupled; DNA; DNA Damage; Databases; Development; Diet; Educational workshop; Environment; Epigenetic Process; Evaluation; Exposure to; Fingerprint; Genetic; Genetic Transcription; Genetic Variation; Genome; Genomics; Goals; Human; Human Genome; Individual; Laboratories; Left; Link; Longevity; Measurement; Measures; Methylation; Modification; Molecular Profiling; National Institute of Environmental Health Sciences; Outcome; Oxidation-Reduction; Pathway Analysis; Pathway interactions; Physiological; Predisposition; Process; Production; Proteins; Research Design; Research Personnel; Resources; Sampling; Science; Targeted Research; Update; Variant; Work; adduct; biological systems; cytokine; editorial; epigenomics; flexibility; indexing; macromolecule; member; metabolomics; microbiome; receptor; response; telomere; working group

The Biological Response Resource (BRR) will examined the interactions of exogenous and endogenous chemicals with our biological molecules as they are necessary for there to be a biological effect. One of the most important and practical reasons to include the biological response is that the vast majority of exposures are transient. The chemicals underlying exposures that occurred weeks, months, or years ago are long gone from the body. However, these past chemical exposures often leave molecular fingerprints of their presence. Whether this be damaged DNA, epigenetic modification, or protein adduct formation, the biological fingerprints may be able to provide information of these transient exposures of the past. Without the specific inclusion of the biological impact of exposures in the working definition of the exposome we are left with no way to assess prior exposures at a biological level. The overall goal of the Biological Response Resource BRR is to provide expertise and access to a suite of validated measures of biological responses. In order to offer maximal flexibility to potential users, our BRR is offering a wide array of assays that range from genomic and epigenomic offerings to measures of redox status, cytokine production, and pathway analysis. The goal will be pursued through the following objectives: Objective 1. To educate investigators on the benefits of biological assays available and assist in study design and sample optimization. Objective 2. To provide state-of-the-art measurements that assess the biological effects of, and susceptibility to, exposures on the human genome. Objective 3. To provide access to a variety of other indices of biological response, such as comprehensive profiling of redox state, multiplexed cytokine assessment, and microbiome characterization. Objective 4. To collaborate with the Untargeted Research Resource to identify biological responses that can be measured as part of the untargeted (metabolomics) analysis. Objective 5. To interface with the Developmental Research Resource and the Targeted Research Resource to identify biological markers which are highly associated with specific exposures. The Biological Response Resource will work with CHEAR clients, NEALE colleagues, and the entire CHEAR network to develop reliable and reproducible indices of environmentally-induced perturbations of biological systems.

生物反应资源(BRR)将研究外源和内源的相互作用 化学物质与我们的生物分子相互作用，因为它们是产生生物效应所必需的。其中一个 包括生物反应在内的最重要和最实际的原因是，绝大多数接触 都是短暂的。几周前、几个月前或几年前暴露的化学物质早已不复存在 从身体上。然而，这些过去的化学暴露往往会留下它们存在的分子指纹。 无论是DNA损伤，表观遗传修饰，还是蛋白质加合物形成，生物指纹 或许能够提供这些过去的短暂曝光的信息。如果没有具体包含 在曝光组的工作定义中，曝露的生物影响我们无法评估 在生物学水平上的先前暴露。生物响应资源BRR的总体目标是提供 专业知识和获得一套经过验证的生物反应测量方法。为了最大限度地提供 为了给潜在用户提供灵活性，我们的BRR提供了从基因组和 表观基因组提供氧化还原状态的测量、细胞因子的产生和途径分析。目标将是 通过下列目标进行追求：目标1.教育调查人员有关生物的益处 提供分析方法并协助研究设计和样品优化。目标2.提供最先进的 评估暴露在人类基因组上的生物效应和易感性的测量。 目标3.提供各种其他生物反应指数，如综合 氧化还原状态的简档、多重细胞因子评估和微生物组特征。目标4.达到 与非目标研究资源协作，以确定可测量的生物反应 非靶向(代谢组学)分析的一部分。目标5.与发展研究对接 资源和有针对性的研究资源，以确定与 具体的曝光。生物响应资源将与CHEAR客户、Neale同事和 整个CHEAR网络，以开发可靠和可重复的环境诱导指数 生物系统的扰动。