Q-ToF Mass Spectrometer for the University of Utah MS and Proteomics Core

 描述(由申请人提供)：本提案是为犹他大学质谱学和蛋白质组学核心设施Q-ToF Maxis HD质谱仪(Bruker Daltonics)提供资金的申请。该仪器将用于两个主要目的：1)用提供先进研究能力的高性能仪器取代两个旧的、低性能的ESI/MS和不起作用的质谱仪；2)提高质谱学和蛋白质组学核心的样品分析效率和能力，每年约有80至90名犹他大学的研究人员使用。来自犹他大学的10个主要用户，以及NIH资助的19个有效赠款，已被确定为当前和未来的研究项目，这些项目确保了Maxis HD仪器的立即和长期继续使用。另外还有大约70到75名未成年人用户，他们都来自犹他大学。这组主要用户将利用Maxis HD的MS和MS/MS数据的高质量精度能力。主要用户的研究项目各不相同，但应用包括：蛋白质组学(蛋白质ID和蛋白质PTM的发现)，蛋白质序列确认和从完整蛋白质分子中识别蛋白质水解性切点，修饰多肽(天然和合成)的结构表征，药物和其他合成小分子的结构表征，RNA分析和其他核酸研究，以及合成肽/药物偶联结构工作。Q-ToF Maxis HD是一款高性能质谱仪，可为MS和MS/MS应用提供高灵敏度、高质量精度、高分辨率和多功能性，但同时它操作相对简单，是一款可靠的主力质谱仪，非常适合质谱学核心设施的环境。Q-ToF Maxis HD仪器将安装在MS和蛋白质组学核心设施中，这是一个中央质谱学共享资源。MS核心由研究所管理，在一个结构良好的核心研究设施框架内，包括监督和咨询委员会。有非常强大的机构财政支持，以确保仪器的长期使用，包括服务协议和操作仪器的全职博士质谱学家。对犹他大学和地区机构的长期影响将是为大学科学和医学研究界增加新的质谱学资源。这一最先进的质谱学将在未来多年激发新的研究项目和创新。Maxis HD仪器的分析能力将在加速许多领域的研究方面发挥重要作用，特别是蛋白质组学、天然产品研究以及该大学正在研究的许多药物输送系统。

 DESCRIPTION (provided by applicant): This proposal is a request for funding for a Q-ToF Maxis HD mass spectrometer (Bruker Daltonics) for the University of Utah Mass Spectrometry and Proteomics Core facility. The instrument will serve two primary purposes: 1) replacement of two old, low-performance ESI/MS, and non-functional mass spectrometers with a high-performance instrument that provides advanced research capabilities, and 2) increase sample analysis efficiency and capacity for the Mass Spectrometry and Proteomics Core for approximately 80 to 90 investigators at the University of Utah, annually. Ten major users from the University of Utah, with 19 active NIH-funded grants, have been identified with current and future research projects, which ensures immediate and long-term continued use of the Maxis HD instrument. There are about 70 to 75 additional minor users, all from the University of Utah. The group of major users will utilize the high mass accuracy capabilities for MS and MS/MS data of the Maxis HD. The research projects of the major users vary widely, but applications include: Proteomics (protein ID and protein PTM discovery), protein sequence confirmations and identification of proteolytic cut sites from intact protein molecules, structural characterizaton of modified peptides (natural and synthetic), structural characterization of drugs and other synthetic small molecules, RNA analysis and other nucleic acid investigations, and synthetic peptide/drug conjugate structure work. The Q-ToF Maxis HD is a high-performance mass spectrometer that provides high sensitivity, high mass accuracy, high resolving power, and versatility for both MS and MS/MS applications, but at the same time, it's relatively easy to operate and is a dependable, workhorse mass spectrometer that is well-suited for the environment of a mass spectrometry core facility. The Q-ToF Maxis HD instrument will be housed in the MS and Proteomics Core facility, a central mass spectrometry shared resource. The MS Core is administered by the Institution, within a well-structured Core Research Facilities framework, including Oversight and Advisory committees. There is very strong Institutional financial support to ensure long-term utilization of the instrument, including service agreements and full-time Ph.D. mass spectrometrists to operate the instrument. The long-term impact to the University of Utah and regional institutions will be addition of new mass spectrometry resources for the University scientific and medical research community. This state-of-the-art mass spectrometry will stimulate new research projects and innovations for many years. The analytical power of the Maxis HD instrument will play a major role in accelerating research in many areas, particularly, proteomics, natural-product research, and many drug-delivery systems under investigation at the University.