Metabolomics Core (Core C)

项目摘要 代谢组学核心将提供生物构建模块，氨基酸， 核苷酸，脂质和碳水化合物的铁和血红素疾病中心（CIHD）的研究人员。 代谢组学是对组织、生物液体、生物体、培养物或组织中发现的代谢物的无偏调查。 其他生物来源。目前，代谢组学是一门比较科学;通常进行分析， 识别在已经进行处理的生物样品之间发现的差异，或者 条件这可能是基因突变，药物治疗或环境限制。没有一种方法是完全 能够完全分析细胞或组织的代谢组。使代谢物的数量最大化 据观察，核心配备了三个化学分析平台，气相色谱-质谱 光谱法、液相色谱-质谱法和核磁共振法 谱CIHD的研究基础集中在可以很容易地解决这些问题， 服务拟议的代谢组学核心的工作人员将协助研究者进行样本 准备、设备使用和分析。方案工作人员还将协助用户准备材料， 在CIHD内部和外部的国家和国际会议上发表和演讲。

PROJECT SUMMARY   The Metabolomics Core will provide analytic measurements of biologic building blocks, amino acids, nucleotides, lipids and carbohydrates to investigators of the Center for Iron and Heme Disorders (CIHD). Metabolomics is the unbiased survey of metabolites found within a tissue, biological fluid, organism, culture or other biological source. Currently metabolomics is a comparative science; typically an analysis is performed to identify the differences found between biological samples that have been subjected to a treatment or condition. This can be a genetic mutation, drug treatment or environmental constraint. No one method is fully capable of completely profiling the metabolome of a cell or tissue. To maximize the number of metabolites observed, the Core is equipped with three chemical analysis platforms, Gas Chromatography-Mass Spectrometry, Liquid Chromatography-Mass Spectrometry, and Nuclear Magnetic Resonance spectroscopy. The research base of the CIHD focuses on questions that can easily be addressed with these services. The staff of the proposed Metabolomics Core will assist investigators in training sessions for sample preparation, equipment use and analysis. Program staff will also assist users in preparation of materials for publications and presentations within the CIHD and externally to national and international meetings.