Agilent 6550 QTOF system for U of Utah

适用于犹他大学的 Agilent 6550 QTOF 系统

• 批准号: 8447176
• 负责人: JAMES Eric COX
• 金额: $ 57.8万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447176
• 关键词: Basic Science; Complement; Data; Disease; Funding; Gas Chromatography; Hematology; Liquid Chromatography; Mass Spectrum Analysis; Metabolism; Metals; Mitochondrial Proteins; Molecular; Mutation; National Institute of Diabetes and Digestive and Kidney Diseases; Patients; Publications; System; Time; United States National Institutes of Health; Universities; Utah; Water; base; improved; instrument; mass spectrometer; metabolomics; microbial alkaline proteinase inhibitor; success

DESCRIPTION (provided by applicant): The Metabolomics Core at the University of Utah was initiated in 2006 with funding from NIH/NIDDK in the form of a Centers of Excellence in Molecular Hematology. From its inception the Core has performed non-targeted analysis of the metabolome using gas-chromatography based mass spectrometry (GC-MS). The Core initially focused on the relationship of metals in metabolism, searching for the underlying causes of disease states when key metal metabolites were either in excess or limiting. It has broadened its focus to other disease, especially those caused by mutations in mitochondrial proteins. The Core has been highly successful in this endeavor with resulting data has been included in a number of publications. Not all metabolites are detected using this GC-MS based approach. The Core over the past year has used an older PE SCIEX API 365 triple quadrupole LC-MS/MS to perform targeted metabolomics with success. Unfortunately this instrument suffers from major limitations; it is older, unreliable and does not perform non-targeted analysis. This instrument needs to be replaced with a modern, high-end instrument which has increased sensitivity, greater dynamic range and importantly improved reliability. We propose the purchase of an Agilent 6550 Quadrupole-Time-Of-Flight (QTOF) mass spectrometer with an Agilent 1290 Infinity ultrapressure liquid chromatography system. This system will complement our current Waters GCT-Premier GC-MS and will be used immediately at full capacity. We are pursuing a number of medically related metabolomics projects currently, from basic research in understanding the causes of disease to actual patient studies.

描述（由申请人提供）：犹他大学代谢组学核心于 2006 年启动，由 NIH/NIDDK 以分子血液学卓越中心的形式提供资金。从一开始，Core 就使用基于气相色谱的质谱法 (GC-MS) 对代谢组进行非靶向分析。核心最初关注金属在新陈代谢中的关系，寻找当关键金属代谢过多或限制时疾病状态的根本原因。它已将其关注范围扩大到其他疾病，尤其是由线粒体蛋白突变引起的疾病。 Core 在这项工作中取得了巨大成功，所得数据已包含在许多出版物中。使用这种基于 GC-MS 的方法并不能检测到所有代谢物。在过去的一年中，Core 使用较旧的 PE SCIEX API 365 三重四极杆 LC-MS/MS 成功执行了目标代谢组学。不幸的是，这个工具有很大的局限性。它比较旧、不可靠并且不执行非目标分析。该仪器需要更换为现代化的高端仪器，该仪器具有更高的灵敏度、更大的动态范围以及显着提高的可靠性。我们建议购买配备 Agilent 1290 Infinity 超压液相色谱系统的 Agilent 6550 四极杆飞行时间 (QTOF) 质谱仪。该系统将补充我们当前的 Waters GCT-Premier GC-MS，并将立即满负荷使用。我们目前正在开展许多与医学相关的代谢组学项目，从了解疾病原因的基础研究到实际的患者研究。