Tecan Resolvex for University of Utah

犹他大学 Tecan Resolvex

• 批准号: 10734678
• 负责人: JAMES Eric COX
• 金额: $ 5.69万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10734678
• 关键词: Academia; American; Biological; Chemicals; Devices; Diabetes Mellitus; Ensure; Foundations; Funding; Funding Agency; Grant; Human; Industry; Institution; Lipids; Liquid substance; Manuals; Mass Spectrum Analysis; Methodology; Methods; Modernization; Pesticides; Pharmaceutical Preparations; Phase; Preparation; Process; Request for Proposals; Research Personnel; Sampling; Services; Solid; Solvents; Technology; Time; Tracer; United States Department of Agriculture; United States National Institutes of Health; Universities; Utah; Work; data quality; human error; lipidomics; metabolomics; pressure

Project Summary / Abstract This proposal requests funding for a Tecan Resolvex A200 (Resolvex) to the Metabolomics Core (Core) for two primary purposes, to: 1) increase sample processing throughput, and 2) decrease human sample processing error. The Core provides a wide array of mass spectrometry-based services including metabolomics, lipidomics, flux tracer analysis, and targeted metabolite/drug quantitation. Over the past four fiscal years, the Core has served an average of 53 Principle Investigators (PI) per year, 40 PI’s from 20 departments at the University of Utah (UU) and an average of 13 users from outside academic institutions. The Core only tracks funding sources from UU researchers with an average of 23 NIH grants served per year. Other major funding sources include the American Diabetes Association, DOD, HHMI, JDRF, NSF, USDA as well as other foundation grants. The Core currently process approximately 10,000 samples a year, with most extractions performed manually using liquid/liquid extraction methods. This work is tedious, low throughput and prone to human error. Solid phase extraction (SPE) methodology has long standing in both academia and industry for the extraction of drugs and pesticides from biological matrixes. Recently, several technologies that use SPE for metabolomics and lipidomics have been introduced by Agilent. These technologies serve two functions, 1) they remove interfering chemical entities from a sample matrix, and 2) due to their 96-well format, increase sample through put. In order to meet the ever-increasing demand for Core services, a high throughput modernization plan is necessary. The Resolvex is central to this, it is needed to perform automated sample wash and elution steps from 96-well SPE plates by the precise addition of solvents to each well. The positive pressure mechanism used to elute solvents from each well ensures uniform sample preparation. Without this device, it is very difficult to leverage all the advantages provided by these new SPE technologies. The Resolvex is central to the modernization plan of the Core and will immediately make an impact in both sample throughput and data quality. It will be immediately used for the extraction and purification of both metabolites and lipids. In addition, by limiting the human error associated by manual pipetting, it will make a considerable difference in data quality.

项目摘要 /摘要 该建议要求为Tecan Resolvex A200（resolvex）提供资金 两个主要目的：1）增加样品加工吞吐量，2）减少人类样本 处理错误。 该核心提供了各种基于质谱的服务，包括代谢组学，脂质组学，助剂 示踪剂分析和靶向代谢物/药物定量。在过去的四个财政年度中，核心已经服务 每年平均有53名主要调查人员（PI），犹他大学的20个部门来自20个PI （UU）和来自外部学术机构的13位用户平均。核心仅跟踪资金来源 来自UU的研究人员，平均每年提供23个NIH赠款。其他主要资金来源包括 美国糖尿病协会，国防部，HHMI，JDRF，NSF，USDA以及其他基金会赠款。这 目前，核心每年处理大约10,000个样本，大多数提取 液体/液体提取方法。这项工作是乏味的，低的吞吐量，容易发生人为错误。 固相提取（SPE）方法论在学术界和行业中长期以来提取提取 来自生物基质的药物和农药。最近，几种使用SPE的技术 Agilent引入了代谢组学和脂肪组学。这些技术发挥了两个功能，1） 从样品矩阵中删除干扰化学实体，而2）由于其96孔格式，增加了样品 通过。为了满足对核心服务不断增长的需求，高通量现代化 计划是必要的。 Resolvex对此至关重要，需要进行自动样品洗涤和洗脱 通过向每个孔中添加溶液，从96孔SPE板上的台阶。正压 用于洗脱溶液的机制可确保样品制备均匀。没有这个设备，它是 很难利用这些新SPE技术提供的所有优势。 Resolvex是核心现代化计划的核心，并将立即对两者产生影响 样品吞吐量和数据质量。它将立即用于提取和纯化 代谢物和脂质。此外，通过限制手动移液相关的人为错误，它将成为一个 数据质量的差异很大。