Tecan Resolvex for University of Utah

项目总结/摘要 该提案要求为Tecan Resolvex A200（Resolvex）向代谢组学核心（核心）提供资金， 两个主要目的：1）增加样本处理量，2）减少人类样本 处理错误。 Core提供广泛的基于质谱的服务，包括代谢组学、脂质组学、通量 示踪剂分析和靶向代谢物/药物定量。在过去的四个财政年度里， 平均每年有53名主要研究者（PI），来自犹他州大学20个系的40名PI (UU)平均有13个用户来自学术机构以外。核心只跟踪资金来源 来自UU研究人员，平均每年提供23项NIH赠款。其他主要资金来源包括 美国糖尿病协会，国防部，HHMI，JDRF，NSF，USDA以及其他基金赠款。的 Core目前每年处理约10，000个样本，大多数提取都是使用 液/液萃取法。这项工作是繁琐的，低吞吐量和容易出现人为错误。 固相萃取（SPE）技术在工业和学术界都有着悠久的历史 从生物基质中提取药物和杀虫剂。最近，几种使用SPE进行 代谢组学和脂质组学已经由Agilent引入。这些技术有两个功能，1）它们 从样品基质中去除干扰化学实体，和2）由于其96孔格式，增加样品 通过put。为了满足日益增长的核心服务需求， 计划是必要的。Resolvex是这方面的核心，它需要执行自动样品洗涤和洗脱 通过向每个孔中精确加入溶剂，从96孔SPE板中分离步骤。正压 用于从每个孔中除去溶剂的机械装置确保了样品制备的均匀性。如果没有这个装置， 很难充分利用这些新SPE技术提供的所有优势。 Resolvex是核心现代化计划的核心，并将立即对两者产生影响。 样品吞吐量和数据质量。它将立即用于提取和纯化 代谢物和脂质。此外，通过限制与手动移液相关的人为错误，它将使 数据质量有很大差异。

Project Summary / Abstract This proposal requests funding for a Tecan Resolvex A200 (Resolvex) to the Metabolomics Core (Core) for two primary purposes, to: 1) increase sample processing throughput, and 2) decrease human sample processing error. The Core provides a wide array of mass spectrometry-based services including metabolomics, lipidomics, flux tracer analysis, and targeted metabolite/drug quantitation. Over the past four fiscal years, the Core has served an average of 53 Principle Investigators (PI) per year, 40 PI’s from 20 departments at the University of Utah (UU) and an average of 13 users from outside academic institutions. The Core only tracks funding sources from UU researchers with an average of 23 NIH grants served per year. Other major funding sources include the American Diabetes Association, DOD, HHMI, JDRF, NSF, USDA as well as other foundation grants. The Core currently process approximately 10,000 samples a year, with most extractions performed manually using liquid/liquid extraction methods. This work is tedious, low throughput and prone to human error. Solid phase extraction (SPE) methodology has long standing in both academia and industry for the extraction of drugs and pesticides from biological matrixes. Recently, several technologies that use SPE for metabolomics and lipidomics have been introduced by Agilent. These technologies serve two functions, 1) they remove interfering chemical entities from a sample matrix, and 2) due to their 96-well format, increase sample through put. In order to meet the ever-increasing demand for Core services, a high throughput modernization plan is necessary. The Resolvex is central to this, it is needed to perform automated sample wash and elution steps from 96-well SPE plates by the precise addition of solvents to each well. The positive pressure mechanism used to elute solvents from each well ensures uniform sample preparation. Without this device, it is very difficult to leverage all the advantages provided by these new SPE technologies. The Resolvex is central to the modernization plan of the Core and will immediately make an impact in both sample throughput and data quality. It will be immediately used for the extraction and purification of both metabolites and lipids. In addition, by limiting the human error associated by manual pipetting, it will make a considerable difference in data quality.