X-Ray Crylatllography

X射线晶体分析法

• 批准号: 9071332
• 负责人: KAREN E KNUDSEN
• 金额: $ 7.89万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9071332
• 关键词: Affinity; Binding; Biochemistry; Biological; Biological Assay; Biosensor; Calorimetry; Catalysis; Cells; Chromatography; Collaborations; Computer Workstations; Computer software; Consultations; Crystallization; Crystallography; Data; Data Analyses; Devices; Diffusion; Dissociation; Drops; Entropy; Equilibrium; Equipment; Exclusion; Experimental Designs; Fee-for-Service Plans; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Free Energy; Grant; Heating; Housing; Instruction; Kinetics; Macromolecular Complexes; Malignant Neoplasms; Measurement; Measures; Methods; Minor; Molecular; Molecular Structure; Optics; Phase; Production; Property; Reaction; Reporting; Research Personnel; Resolution; Robot; Roentgen Rays; Sampling; Sedimentation process; Services; Source; Staging; Structure; Surface Plasmon Resonance; System; Technical Expertise; Technology; Temperature; Thermodynamics; Time; Titrations; Training; Translational Research; Tube; United States National Institutes of Health; X ray diffraction analysis; X-Ray Crystallography; base; biophysical techniques; chemical reaction; computerized data processing; computing resources; conformational alteration; data acquisition; enthalpy; instrument; instrumentation; member; millisecond; nano; nanoinstrument; research study; screening; seal; sedimentation velocity; tumor progression

PROJECT SUMMARY (See instructions): The X-ray Crystallography and Molecular Characterization Facility provides access to state-of-the-art x-ray crystallography instrumentation and computational resources, and a wide variety of biophysical approaches lo understanding important molecular interactions. The Facility maintains the equipment and technical expertise to carry out all stages of macromolecular structure determination, and to measure molecular binding parameters such as association and dissociation rates as well as secondary and tertiary structure of biological samples. This facility was used by 6 KCC members.

项目概述（见说明）：x射线晶体学和分子表征设施提供了最先进的x射线晶体学仪器和计算资源，以及各种各样的生物物理方法来理解重要的分子相互作用。该设施拥有进行大分子结构测定的所有阶段的设备和技术专长，并测量分子结合参数，如结合和解离率以及生物样品的二级和三级结构。该设施由6名KCC成员使用。