Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

摘要 自1999年成立以来，CHAMACOS研究是运行时间最长的队列研究之一 早期生活环境暴露对神经发育、生长和呼吸系统疾病的影响 唯一的一次关注的是农场工人人口中的低收入拉丁裔儿童。我们收集了大量的 健康、暴露、人口、邻里和地区数据，以及生物学数据(如血液、尿液、 母乳、头发、唾液、乳牙)和环境(如粉尘、过敏原)样本 已经创建了一个大型生物储存库，存储了超过22万个样本以备将来使用。有140多个 CHAMACOS是一个成功的、久负盛名的环境流行病学队列。 我们使用保存的标本和存档数据来证明出生前和出生后的关系 暴露在杀虫剂、阻燃剂和其他神经发育较差的化学品中，会降低肺部 功能、肥胖和其他结果。我们已经证明，环境暴露会影响许多 影响健康的分子机制，如PON1酶活性、脂肪因子和异前列腺素 水平、DNA甲基化和miRNA表达。CHAMACOS资源支持多个NIH， 环境保护局，以及非联邦赠款和受训人员，包括与其他机构的合作。然而， 管理这一海量数据所需的基础设施，确保安全的实验室设施 数十万个样本，以及保持家庭参与和参与这一长期- 随着时间的推移，正在进行的研究增加了，而这些活动的资金却减少了。 这项建议的目的是(1)通过社区参与保持对队列的参与，(2) 维护和加强数据管理基础设施；(3)维护和加强现有生物储存库 通过更换、维修和维护老化的冷冻箱，以确保样品和 对系统的改进，以跟踪用于多项研究资助、试点研究和合作的样本， (4)开展验证、试点和可行性研究，研究环境评估的新方法 暴露，开发生物资源库的最佳实践，并探索与以下相关的新方法 代谢组学、基因组学和微生物组学，以及(5)开发数据共享门户网站，以鼓励 现有CHAMACOS数据的外部合作者。 总之，对CHAMACOS队列研究的基础设施支持将保存标本，确保- 记录数据以供未来研究和数据共享，并保持参与者保留，确保有效 这些宝贵资源的未来用途。因此，这笔赡养费将增强我们回答 关于环境暴露对生命过程中健康的影响的关键问题，并将确保 CHAMACOS研究产生的广泛资源将被调查人员有效地利用 在未来的几年里，在全世界范围内。

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 220,000 samples stored for future use. With over 140 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non-federal grants and trainees, including collaborations with other institutions. However, the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long- running study have increased over time, while funding for these activities has decreased. The aims for this proposal are to (1) retain participation in the cohort through community engagement, (2) maintain and strengthen data management infrastructure, (3) maintain and enhance the existing biorepository through replacement, repair, and maintenance of aging deep freezers to ensure the integrity of samples and improvements to systems to track samples used for multiple research grants, pilot studies, and collaborations, (4) conduct validation, pilot, and feasibility studies to investigate new methods of assessing environmental exposures, develop best practices for biorepositories, and explore novel methodologies related to metabolomics, genomics, and the microbiome, and (5) develop a data sharing portal to encourage use by outside collaborators of existing CHAMACOS data. In summary, infrastructure support for the CHAMACOS cohort study will preserve specimens, ensure well- documented data for future studies and data sharing, and maintain participant retention, assuring effective future use of these valuable resources. This maintenance grant will thereby strengthen our ability to answer key questions about the impact of environmental exposures on health over the life course and will assure that the extensive resources generated by the CHAMACOS study will be effectively used by investigators worldwide for years to come.