Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

为子孙后代维护和扩展 CHAMACOS 流行病学队列基础设施

• 批准号: 10200041
• 负责人: Brenda Eskenazi
• 金额: $ 29.05万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10200041
• 关键词: 1 year old; 14 year old; 21 year old; Adolescence; Adult; Affect; Aging; Agriculture; Allergens; Arylesterase; Back; Big Data; Biological; Biological Markers; Biological Specimen Banks; Biological Specimen database; Birth; Blood; California; Chemicals; Child; Cohort Studies; Collaborations; Communication; Communities; DNA Methylation; Data; Data Management Resources; Database Management Systems; Databases; Development; Documentation; Dust; Ensure; Environmental Epidemiology; Environmental Exposure; Environmental Impact; Epidemiology; Exposure to; Family; Feasibility Studies; Flame Retardants; Freezing; Funding; Future; Future Generations; Genomics; Grant; Growth; Hair; Health; Human Milk; Infrastructure; Institution; International; Isoprostanes; Knowledge; Latino; Life; Life Cycle Stages; Low income; Lung diseases; Maintenance; Methodology; Methods; MicroRNAs; Modeling; Molecular; Neighborhoods; Newsletter; Obesity; Outcome; Paper; Participant; Pesticides; Pilot Projects; Population; Protocols documentation; Publications; Publishing; Reporting; Research; Research Personnel; Research Project Grants; Resources; Running; Safety; Salinas Valley; Saliva; Sampling; Scientific Advances and Accomplishments; Specimen; Standardization; System; Time; United States National Institutes of Health; Urine; Visit; Youth; adipokines; agricultural community; archive data; archived data; biobank; cohort; community based participatory research; community engagement; data management; data portal; data sharing; deciduous tooth; early life exposure; early pregnancy; farm worker; improved; laboratory facility; metabolomics; microbiome; neurodevelopment; novel; participant retention; postnatal; preservation; pulmonary function; repaired; validation studies; young adult

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 220,000 samples stored for future use. With over 140 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non-federal grants and trainees, including collaborations with other institutions. However, the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long- running study have increased over time, while funding for these activities has decreased. The aims for this proposal are to (1) retain participation in the cohort through community engagement, (2) maintain and strengthen data management infrastructure, (3) maintain and enhance the existing biorepository through replacement, repair, and maintenance of aging deep freezers to ensure the integrity of samples and improvements to systems to track samples used for multiple research grants, pilot studies, and collaborations, (4) conduct validation, pilot, and feasibility studies to investigate new methods of assessing environmental exposures, develop best practices for biorepositories, and explore novel methodologies related to metabolomics, genomics, and the microbiome, and (5) develop a data sharing portal to encourage use by outside collaborators of existing CHAMACOS data. In summary, infrastructure support for the CHAMACOS cohort study will preserve specimens, ensure well- documented data for future studies and data sharing, and maintain participant retention, assuring effective future use of these valuable resources. This maintenance grant will thereby strengthen our ability to answer key questions about the impact of environmental exposures on health over the life course and will assure that the extensive resources generated by the CHAMACOS study will be effectively used by investigators worldwide for years to come.

抽象的 自1999年成立以来，Chamacos研究是检查最长的研究队列研究之一。 早期生活环境暴露对神经发育，生长和呼吸系统疾病的影响以及 唯一专注于农民人口中低收入的拉丁裔儿童。我们收集了广泛的 健康，暴露，人口统计，邻里和区域数据以及生物学（例如血液，尿液， 母乳，头发，唾液，落叶牙齿）和环境（例如灰尘，过敏原）在多次访问和 已经创建了一个大型生物座，并存储了220,000多个样品供将来使用。超过140 出版物Chamacos是一个成功且建立了良好的环境流行病学队列。 我们已经使用了银行标本和存档数据来证明产后和产后的关系 暴露于农药，阻燃剂和其他神经发育较差的化学物质，肺部降低 功能，肥胖和其他结果。我们已经表明，环境暴露会影响众多 影响健康的分子机制，例如PON1酶活性，脂肪因子和异丙烷 水平，DNA甲基化和miRNA表达。 Chamacos资源支持了多个NIH， EPA和非联邦赠款和学员，包括与其他机构的合作。但是， 管理这一庞大数据所需的基础设施，实验室设施，以确保安全 成千上万的样本，以及使家庭参与并参与这一长期的努力 随着时间的流逝，跑步研究的增加，而这些活动的资金减少了。 该建议的目的是（1）通过社区参与保留参与队列，（2） 维护和加强数据管理基础架构，（3）维护和增强现有的生物座席 通过更换，维修和维护老化的深冷冻机，以确保样本的完整性和 改进系统，以跟踪用于多种研究补助，试点研究和协作的样本， （4）进行验证，飞行员和可行性研究以研究评估环境的新方法 暴露，开发生物焦点的最佳实践，并探索与 代谢组学，基因组学和微生物组，（5）开发一个数据共享门户，以鼓励使用 现有Chamacos数据的外部合作者。 总而言之，Chamacos队列研究的基础设施支持将保留标本，确保井井有条 记录了未来研究和数据共享并保持参与者保留的数据，并确保有效 这些宝贵资源的未来使用。因此，这种维护赠款将增强我们回答的能力 关于环境暴露对健康过程中健康状况影响的关键问题，并将确保 调查员将有效地使用Chamacos研究产生的广泛资源 在未来的几年中，全球范围内。