Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

摘要 自1999年成立以来，CHAMACOS研究是最长的队列研究之一， 生命早期环境暴露对神经发育、生长和呼吸系统疾病的影响， 唯一一个关注农场工人中的低收入拉丁裔儿童。我们收集了大量 健康、暴露、人口统计、邻近和区域数据，以及生物（例如，血液，尿液， 多次访视时的母乳、毛发、唾液、乳牙）和环境（如灰尘、过敏原）样本， 已经建立了一个大型生物储存库，储存了22万多个样本供未来使用。带140个 CHAMACOS是一个成功和完善的环境流行病学队列。 我们使用了库存标本和存档数据来证明产前和产后的关系。 暴露于杀虫剂、阻燃剂和其他化学物质，神经发育较差，肺功能下降， 功能、肥胖和其他结果。我们已经表明，环境暴露会影响许多 影响健康的分子机制，如PON 1酶活性，脂肪因子和异前列腺素 水平、DNA甲基化和miRNA表达。CHAMACOS资源支持多个NIH， 环境保护局，和非联邦赠款和学员，包括与其他机构的合作。但 管理这一巨大数据宝藏所需的基础设施，实验室设施，以确保安全 成千上万的样本，以及努力让家庭参与和参与这一长期的- 随着时间的推移，进行研究的人数增加了，而对这些活动的供资却减少了。 该提案的目的是（1）通过社区参与保持对队列的参与，（2） 维护和加强数据管理基础设施，（3）维护和加强现有的生物储存库 通过更换、修理和维护老化的深冻机，确保样品的完整性， 改进用于跟踪多项研究赠款、试点研究和合作的样本的系统， (4)进行验证，试点和可行性研究，以调查评估环境的新方法 暴露，开发生物储存库的最佳实践，并探索与以下方面相关的新方法 代谢组学、基因组学和微生物组学，以及（5）开发一个数据共享门户网站，以鼓励 现有CHAMACOS数据的外部合作者。 总之，CHAMACOS队列研究的基础设施支持将保存标本，确保良好- 记录数据，用于未来的研究和数据共享，并保持参与者的保留，确保有效 这些宝贵资源的未来。这笔生活补助金将加强我们的能力， 关于环境暴露对生命过程中健康影响的关键问题，并将确保 CHAMACOS研究所产生的大量资源将被研究人员有效地利用 在未来的几年里，世界各地。

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 220,000 samples stored for future use. With over 140 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non-federal grants and trainees, including collaborations with other institutions. However, the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long- running study have increased over time, while funding for these activities has decreased. The aims for this proposal are to (1) retain participation in the cohort through community engagement, (2) maintain and strengthen data management infrastructure, (3) maintain and enhance the existing biorepository through replacement, repair, and maintenance of aging deep freezers to ensure the integrity of samples and improvements to systems to track samples used for multiple research grants, pilot studies, and collaborations, (4) conduct validation, pilot, and feasibility studies to investigate new methods of assessing environmental exposures, develop best practices for biorepositories, and explore novel methodologies related to metabolomics, genomics, and the microbiome, and (5) develop a data sharing portal to encourage use by outside collaborators of existing CHAMACOS data. In summary, infrastructure support for the CHAMACOS cohort study will preserve specimens, ensure well- documented data for future studies and data sharing, and maintain participant retention, assuring effective future use of these valuable resources. This maintenance grant will thereby strengthen our ability to answer key questions about the impact of environmental exposures on health over the life course and will assure that the extensive resources generated by the CHAMACOS study will be effectively used by investigators worldwide for years to come.