Long-term sequelae of early life pesticide exposure in the CHAMACOS birth cohort

CHAMACOS 出生队列中生命早期接触农药的长期后遗症

• 批准号: 9899747
• 负责人: Brenda Eskenazi
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-21 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9899747
• 关键词: 16 year old; 18 year old; Accounting; Address; Adolescence; Adult; Age; Agriculture; Attention; Barker Hypothesis; Biological; Biological Assay; Biological Markers; Birth; Blood; Blood Cells; Blood specimen; Brain; California; Cardiovascular Diseases; Cardiovascular system; Chemical Exposure; Chemicals; Child; Childhood; Clinical Chemistry; Cohort Studies; Collection; Communities; DNA Methylation; Data; Data Collection; Data Reporting; Development; Diabetes Mellitus; Enrollment; Environmental Exposure; Environmental Policy; Epigenetic Process; Exposure to; Fasting; Fatty Liver; Flame Retardants; Functional Magnetic Resonance Imaging; Generations; Genetic; Growth; Health; Health Policy; Hispanics; Hydroxyl Radical; Hypertension; Immigrant; Individual; Laboratories; Learning; Length; Life; Liver diseases; Longterm Follow-up; Low income; Lung diseases; Measures; Metabolic; Metabolic Diseases; Metabolic syndrome; Methodology; Methods; Mexican; Mexican Americans; Modeling; Modification; Molecular; Monitor; Mothers; National Children' s Study; Near-Infrared Spectroscopy; Neonatal; Neuropsychology; Obesity; Organophosphates; Outcome; Outcome Measure; Overweight; Paper; Participant; Pervasive Development Disorder; Pesticides; Phase; Population; Positioning Attribute; Pregnancy; Process; Protocols documentation; Publishing; Questionnaires; Reflex action; Reporting; Respiratory Signs and Symptoms; Respiratory physiology; Salinas Valley; Sampling; School-Age Population; Ships; Solid; Specimen; Spirometry; Symptoms; Testing; Time; Trust; Umbilical Cord Blood; United States National Institutes of Health; Universities; Urine; Youth; agricultural community; agricultural pesticide; biobank; bisphenol A; cohort; data management; early childhood; early life exposure; emerging adult; executive function; experience; farm worker; follow-up; health assessment; high risk; high risk population; in utero; insight; instrument; multidisciplinary; neurobehavioral; neurodevelopment; neuroimaging; novel; obesity risk; perinatal outcomes; pesticide exposure; phthalates; pollutant; prenatal; prenatal exposure; respiratory; respiratory health; season of birth; sex; tool

ABSTRACT The Center for the Health Assessment of Mothers and Children of Salinas (CHAMACOS) is a longitudinal birth cohort of ~600 primarily first-generation Mexican American children living in the agricultural Salinas Valley, California. CHAMACOS was formed with the same intent as ECHO: to examine the association of early life environmental exposures with birth outcomes, neurodevelopment, respiratory health, and obesity. We have followed children from in utero through adolescence and we now propose to follow them into adulthood. Over the past 17 years, we have developed effective strategies to enroll, track, and retain study participants in this low-income Latino population; we have succeeded in collecting high quality data on all four endpoints of focus for ECHO; we have collected, processed, shipped, and stored over 150,000 varied biological and environmental samples; we have measured over 100 chemical exposures; we have helped develop and test sophisticated and novel exposure, genetic, and epigenetic laboratory assays; and we have developed a solid community-university partnership, earning the trust of participants and community stakeholders. Thus, CHAMACOS could serve as a model birth cohort for ECHO, rare in its length and breadth of assessment of children from birth to adulthood, offering valuable methodologies, lessons learned, and opportunities to the larger ECHO consortium. In addition, CHAMACOS contributes to ECHO a select, high-risk population of Latino children in a farmworker community . As 60% of this cohort is overweight or obese, we also offer data on children at high risk of metabolic, cardiovascular, and liver disease. Because the CHAMACOS children are approaching adulthood, we are uniquely positioned to examine the early origins of adult disease and address important gaps in the relationship between early life exposures and multiple health endpoints. We have reported that in utero organophosphate pesticide (OP) exposure is associated with poorer attention and executive function, lower IQ, respiratory symptoms, and poorer lung function at school-age. In the UG3 phase, we aim to test new methods that will expand our understanding of these relationships and contribute cutting-edge methodologies to ECHO. We propose to: 1) examine a newly-studied epigenetic modification, hydroxyl-methylcytosine (5-hmC) – a potentially strong candidate for understanding the molecular mechanisms underlying effects on neurodevelopment; and 2) apply near infrared spectroscopy (fNIRs) for functional neuroimaging – an inexpensive and convenient alternative to functional MRI. In the UH3 phase, we propose to follow-up participants at age 18 and 21 to assess the effects of in utero and early childhood exposure to agricultural pesticides on: 1) neurobehavioral endpoints; 2) respiratory symptoms and function; and 3) body mass and related metabolic and cardiovascular conditions. This valuable cohort has the potential to add rich data, validated protocols, and practical experience to the ECHO consortium, while also answering vital questions about the long-term sequelae of prenatal and early life exposure to pesticides on adult health.

摘要 萨利纳斯母亲和儿童健康评估中心(CHAMACOS)是一个纵向的 生活在农业萨利纳的大约600名主要是第一代墨西哥裔美国儿童的出生队列 加利福尼亚州山谷。 CHAMACOS的成立与ECHO的目的相同：检查早期 生活环境暴露与出生结局、神经发育、呼吸健康和肥胖的关系。 我们有 从子宫内跟踪儿童到青春期，我们现在建议跟踪他们到成年。 在过去的17年里，我们制定了有效的招生、跟踪和留住学习的策略 低收入拉丁裔人口的参与者；我们成功地收集了所有四个国家的高质量数据 Echo的焦点终端；我们已经收集、处理、运输和存储了超过150,000种不同的 生物和环境样本；我们测量了100多次化学暴露；我们帮助 开发和测试复杂和新颖的暴露、遗传和表观遗传学实验室分析；我们有 建立了稳固的社区-大学合作伙伴关系，赢得了参与者和社区的信任 利益相关者。因此，CHAMACOS可以作为ECHO的模范出生队列，其长度和宽度都是罕见的 对儿童从出生到成年的评估， 提供有价值的方法、经验教训和 给更大的Echo财团带来的机会。此外，CHAMACOS还贡献了 呼应一个精选的、高风险的 农场工人社区中的拉丁裔儿童人口 。由于这一群体中60%的人超重或肥胖，我们还 提供代谢、心血管和肝脏疾病高危儿童的数据。 因为 这个 CHAMACOS 孩子们快要成年了，我们 在研究成人疾病的早期起源方面具有独特的地位 并解决早期生活暴露与多个健康终点之间的关系中的重要差距。 我们已经报道，在子宫内接触有机磷农药(OP)与较差的环境有关 注意力和执行功能，智商降低，呼吸道症状，以及学龄期肺功能较差。在……里面 在UG3阶段，我们的目标是测试新方法，这些方法将扩大我们对这些关系的理解，并 为Echo贡献尖端方法。我们建议：1)研究一种新研究的表观遗传学 修饰，羟甲基胞嘧啶(5-HMC)--一个潜在的理解分子的有力候选者 影响神经发育的潜在机制；以及2)应用近红外光谱(FNIRS) 功能神经成像--一种廉价、方便的替代功能核磁共振的方法。在UH3阶段，我们 建议在18岁和21岁时对参与者进行随访，以评估宫内和儿童早期感染的影响 接触农用农药对：1)神经行为终点；2)呼吸道症状和功能； 3)体重及相关的代谢和心血管疾病。这个有价值的群体有潜力 向ECHO联盟添加丰富的数据、经过验证的协议和实践经验，同时还应 关于产前和早期接触杀虫剂对成人健康的长期后遗症的关键问题。