Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

为子孙后代维护和扩展 CHAMACOS 流行病学队列基础设施

• 批准号: 10166467
• 负责人: Brenda Eskenazi
• 金额: $ 11.38万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10166467
• 关键词: Administrative Supplement; Affect; Agriculture; Allergens; Arylesterase; Biological; Biological Assay; Biological Specimen Banks; Birth; Blood; Chemicals; Child; Cohort Studies; Collaborations; Complex; DNA Methylation; Data; Data Set; Development; Dust; Ensure; Environmental Epidemiology; Environmental Exposure; Environmental Health; Environmental Impact; Epidemiologist; Epidemiology; Epigenetic Process; Exposure to; Faculty; Family; Flame Retardants; Funding; Future; Future Generations; Goals; Grant; Growth; Hair; Health; Housing; Human Milk; Infrastructure; Institution; Isoprostanes; Laboratories; Latino; Life; Life Cycle Stages; Longevity; Low income; Lung diseases; MicroRNAs; Molecular; Neighborhoods; Obesity; Outcome; Participant; Pesticides; Population; Positioning Attribute; Public Health Schools; Publications; Publishing; Research; Research Personnel; Resources; Respiratory physiology; Running; Safety; Salinas Valley; Saliva; Sampling; Secure; Specimen; Statistical Data Interpretation; United States National Institutes of Health; Urine; Validation; Visit; adipokines; biobank; career development; cohort; data archive; data management; data sharing; deciduous tooth; experience; farm worker; functional genomics; improved; laboratory facility; member; neurodevelopment; parent grant; participant retention; postnatal; preservation; skills; student training

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 300,000 samples stored for future use. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non- federal grants and trainees, including collaborations with other institutions. With well over 150 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. The main goals of the parent grant (R24ES028529) were to maintain and improve the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long-running study. However, the School of Public Health (SPH) Biorepository and Children’s Environmental Health Laboratory, which house the biological and environmental specimens has experienced an unexpected shortfall of funding this year. The purpose of this administrative supplement is to provide additional critical funding to the SPH Biorepository. Supplement funds will be used to secure the position of early stage investigator, Dr. Karen Huen, who has been working with the CHAMACOS study for many years. Dr. Huen’s extensive skills and experience at the Children’s Environmental Health Laboratory and SPH Biorepository are crucial for the longevity of the Biorepository and the research that it supports. As an environmental and molecular epidemiologist, Dr. Huen has published extensively on the functional genomics of PON1 and on relationships of environmental exposures with epigenetic profiles in CHAMACOS children. For this supplement, she will support the main goals of the parent grant to maintain the vital functions of the SPH Biorepository. She will continue to train students, researchers, and new faculty members on essential skills for utilizing the invaluable specimens stored at the Biorepository from development and validation of molecular assays to statistical analysis of complex datasets. Further, securing her position will enable us to support her career development as she continues to pursue independent research paths and applies for additional NIH funding in the future.

抽象的 自1999年成立以来，Chamacos研究是检查最长的研究队列研究之一。 早期生活环境暴露对神经发育，生长和呼吸系统疾病的影响以及 唯一专注于农民人口中低收入的拉丁裔儿童。我们收集了广泛的 健康，暴露，人口统计，邻里和区域数据以及生物学（例如血液，尿液， 母乳，头发，唾液，落叶牙齿）和环境（例如灰尘，过敏原）在多次访问和 已经创建了一个大型的生物储物门，其中包含300,000多个样品供将来使用。我们已经使用过 存放标本和存档数据，以证明产后和产后暴露与 农药，阻燃剂和其他神经发育较差的化学物质，肺功能降低， 肥胖和其他结果。我们已经表明，环境暴露会影响多种分子 影响健康的机制，例如PON1酶活性，脂肪因子和异前列腺水平，DNA 甲基化和miRNA表达。 Chamacos资源支持了多个NIH，EPA和非 - 联邦赠款和学员，包括与其他机构的合作。有150多个出版物， Chamacos是一个成功且成熟的环境流行病学队列。 父母赠款（R24ES028529）的主要目标是维护和改善基础设施 管理这一庞大的数据所需的实验室设施，以确保数百个安全 成千上万的样本，以及使家庭参与并参与这项长期研究的努力。 但是，公共卫生学院（SPH）生物座位和儿童环境卫生实验室， 哪个容纳生物和环境标本的资金出乎意料的资金短缺 今年。这种行政补充的目的是为SPH提供更多关键资金 生物座席。补充资金将用于确保早期调查员Karen博士的位置 霍恩（Huen）从事Chamacos研究多年。 Huen博士的广泛技能和 在儿童环境卫生实验室和SPH生物座席的经验对 生物座席的寿命及其支持的研究。作为环境和分子 流行病学家Huen博士广泛发表了PON1的功能基因组学和关系 Chamacos儿童中具有表观遗传特征的环境暴露。对于此补充，她将 支持父母赠款的主要目标，以维持SPH生物座席的重要功能。她会的 继续培训学生，研究人员和新教职员工，了解使用不可估量的基本技能 从分子测定到统计的生物验证中存储在生物座位上的标本 复杂数据集的分析。此外，确保她的职位将使我们能够支持她的职业发展 随着她继续追求独立的研究路径，并在将来申请额外的NIH资金。