Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

为子孙后代维护和扩展 CHAMACOS 流行病学队列基础设施

• 批准号: 10166467
• 负责人: Brenda Eskenazi
• 金额: $ 11.38万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10166467
• 关键词: Administrative Supplement; Affect; Agriculture; Allergens; Arylesterase; Biological; Biological Assay; Biological Specimen Banks; Birth; Blood; Chemicals; Child; Cohort Studies; Collaborations; Complex; DNA Methylation; Data; Data Set; Development; Dust; Ensure; Environmental Epidemiology; Environmental Exposure; Environmental Health; Environmental Impact; Epidemiologist; Epidemiology; Epigenetic Process; Exposure to; Faculty; Family; Flame Retardants; Funding; Future; Future Generations; Goals; Grant; Growth; Hair; Health; Housing; Human Milk; Infrastructure; Institution; Isoprostanes; Laboratories; Latino; Life; Life Cycle Stages; Longevity; Low income; Lung diseases; MicroRNAs; Molecular; Neighborhoods; Obesity; Outcome; Participant; Pesticides; Population; Positioning Attribute; Public Health Schools; Publications; Publishing; Research; Research Personnel; Resources; Respiratory physiology; Running; Safety; Salinas Valley; Saliva; Sampling; Secure; Specimen; Statistical Data Interpretation; United States National Institutes of Health; Urine; Validation; Visit; adipokines; biobank; career development; cohort; data archive; data management; data sharing; deciduous tooth; experience; farm worker; functional genomics; improved; laboratory facility; member; neurodevelopment; parent grant; participant retention; postnatal; preservation; skills; student training

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 300,000 samples stored for future use. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non- federal grants and trainees, including collaborations with other institutions. With well over 150 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. The main goals of the parent grant (R24ES028529) were to maintain and improve the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long-running study. However, the School of Public Health (SPH) Biorepository and Children’s Environmental Health Laboratory, which house the biological and environmental specimens has experienced an unexpected shortfall of funding this year. The purpose of this administrative supplement is to provide additional critical funding to the SPH Biorepository. Supplement funds will be used to secure the position of early stage investigator, Dr. Karen Huen, who has been working with the CHAMACOS study for many years. Dr. Huen’s extensive skills and experience at the Children’s Environmental Health Laboratory and SPH Biorepository are crucial for the longevity of the Biorepository and the research that it supports. As an environmental and molecular epidemiologist, Dr. Huen has published extensively on the functional genomics of PON1 and on relationships of environmental exposures with epigenetic profiles in CHAMACOS children. For this supplement, she will support the main goals of the parent grant to maintain the vital functions of the SPH Biorepository. She will continue to train students, researchers, and new faculty members on essential skills for utilizing the invaluable specimens stored at the Biorepository from development and validation of molecular assays to statistical analysis of complex datasets. Further, securing her position will enable us to support her career development as she continues to pursue independent research paths and applies for additional NIH funding in the future.

抽象的 自 1999 年启动以来，CHAMACOS 研究是运行时间最长的队列研究之一 生命早期环境暴露对神经发育、生长和呼吸系统疾病的影响 唯一一个关注农场工人中低收入拉丁裔儿童的项目。我们收集了大量 健康、暴露、人口、邻里和区域数据，以及生物数据（例如血液、尿液、 多次访视时采集母乳、头发、唾液、乳牙）和环境（例如灰尘、过敏原）样本 创建了一个大型生物样本库，存储了超过 300,000 个样本以供将来使用。我们已经用过 储存样本和存档数据以证明产前和产后接触的关系 农药、阻燃剂和其他化学物质会导致神经发育不良、肺功能下降， 肥胖和其他结果。我们已经证明，环境暴露会影响多种分子 影响健康的机制，例如 PON1 酶活性、脂肪因子和异前列烷水平、DNA 甲基化和 miRNA 表达。 CHAMACOS 资源支持多个 NIH、EPA 和非 联邦拨款和实习生，包括与其他机构的合作。拥有超过 150 种出版物， CHAMACOS 是一个成功且完善的环境流行病学队列。 母基金 (R24ES028529) 的主要目标是维护和改善基础设施 管理如此庞大的数据宝库所需的实验室设施，以确保数百名人员的安全 数千个样本，以及让家庭参与这项长期研究的努力。 然而，公共卫生学院 (SPH) 生物样本库和儿童环境健康实验室， 存放生物和环境标本的地方遭遇了意外的资金短缺 今年。此行政补充的目的是为 SPH 提供额外的关键资金 生物样本库。补充资金将用于确保早期研究员 Karen 博士的职位 Huen 多年来一直致力于 CHAMACOS 研究。禤博士的广泛技能和 儿童环境健康实验室和 SPH 生物样本库的经验对于 生物样本库及其支持的研究的寿命。作为环境和分子 流行病学家 Huen 博士发表了大量有关 PON1 功能基因组学及其关系的文章 CHAMACOS 儿童的环境暴露与表观遗传特征。为了这个补充，她将 支持母基金的主要目标，以维持 SPH 生物样本库的重要功能。她会 继续对学生、研究人员和新教员进行基本技能培训，以利用这些宝贵的知识 从分子测定的开发和验证到统计，样本均储存在生物样本库中 复杂数据集的分析。此外，确保她的职位将使我们能够支持她的职业发展 她将继续追求独立研究道路，并在未来申请额外的 NIH 资助。