Long-term sequelae of early life pesticide exposure in the CHAMACOS birth cohort

CHAMACOS 出生队列中生命早期接触农药的长期后遗症

• 批准号: 9263646
• 负责人: Brenda Eskenazi
• 金额: $ 134.37万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-21 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9263646
• 关键词: 16 year old; 18 year old; Accounting; Address; Adolescence; Adult; Age; Agriculture; Attention; Barker Hypothesis; Biological; Biological Assay; Biological Markers; Birth; Blood; Blood Cells; Blood specimen; Brain; California; Cardiovascular Diseases; Cardiovascular system; Chemical Exposure; Chemicals; Child; Childhood; Clinical Chemistry; Cohort Studies; Collection; Communities; Community Health Centers; DNA Methylation; Data; Data Collection; Data Quality; Data Reporting; Development; Diabetes Mellitus; Enrollment; Environmental Exposure; Environmental Policy; Epigenetic Process; Exposure to; Fasting; Fatty Liver; Flame Retardants; Functional Magnetic Resonance Imaging; Generations; Genetic; Growth; Health; Health Policy; Hispanics; Hydroxyl Radical; Hypertension; Immigrant; Individual; Laboratories; Learning; Length; Life; Liver diseases; Low income; Lung diseases; Measures; Metabolic; Metabolic Diseases; Metabolic syndrome; Methodology; Methods; Mexican; Mexican Americans; Modeling; Modification; Molecular; Monitor; Mothers; National Children' s Study; Near-Infrared Spectroscopy; Neonatal; Obesity; Organophosphates; Outcome; Outcome Measure; Overweight; Paper; Participant; Pervasive Development Disorder; Pesticides; Phase; Population; Positioning Attribute; Pregnancy; Process; Protocols documentation; Publishing; Questionnaires; Reflex action; Reporting; Respiratory Signs and Symptoms; Respiratory physiology; Salinum; Sampling; School-Age Population; Shipping; Ships; Solid; Specimen; Spirometry; Symptoms; Testing; Time; Trust; Umbilical Cord Blood; United States National Institutes of Health; Universities; Urine; Youth; biobank; bisphenol A; cohort; data management; early childhood; early life exposure; emerging adult; executive function; experience; farm worker; follow-up; high risk; in utero; insight; instrument; multidisciplinary; neurobehavioral; neurodevelopment; neuroimaging; neuropsychological; novel; perinatal outcomes; pesticide exposure; phthalates; pollutant; prenatal; prenatal exposure; respiratory; respiratory health; season of birth; sex; tool

ABSTRACT The Center for the Health Assessment of Mothers and Children of Salinas (CHAMACOS) is a longitudinal birth cohort of ~600 primarily first-generation Mexican American children living in the agricultural Salinas Valley, California. CHAMACOS was formed with the same intent as ECHO: to examine the association of early life environmental exposures with birth outcomes, neurodevelopment, respiratory health, and obesity. We have followed children from in utero through adolescence and we now propose to follow them into adulthood. Over the past 17 years, we have developed effective strategies to enroll, track, and retain study participants in this low-income Latino population; we have succeeded in collecting high quality data on all four endpoints of focus for ECHO; we have collected, processed, shipped, and stored over 150,000 varied biological and environmental samples; we have measured over 100 chemical exposures; we have helped develop and test sophisticated and novel exposure, genetic, and epigenetic laboratory assays; and we have developed a solid community-university partnership, earning the trust of participants and community stakeholders. Thus, CHAMACOS could serve as a model birth cohort for ECHO, rare in its length and breadth of assessment of children from birth to adulthood, offering valuable methodologies, lessons learned, and opportunities to the larger ECHO consortium. In addition, CHAMACOS contributes to ECHO a select, high-risk population of Latino children in a farmworker community . As 60% of this cohort is overweight or obese, we also offer data on children at high risk of metabolic, cardiovascular, and liver disease. Because the CHAMACOS children are approaching adulthood, we are uniquely positioned to examine the early origins of adult disease and address important gaps in the relationship between early life exposures and multiple health endpoints. We have reported that in utero organophosphate pesticide (OP) exposure is associated with poorer attention and executive function, lower IQ, respiratory symptoms, and poorer lung function at school-age. In the UG3 phase, we aim to test new methods that will expand our understanding of these relationships and contribute cutting-edge methodologies to ECHO. We propose to: 1) examine a newly-studied epigenetic modification, hydroxyl-methylcytosine (5-hmC) – a potentially strong candidate for understanding the molecular mechanisms underlying effects on neurodevelopment; and 2) apply near infrared spectroscopy (fNIRs) for functional neuroimaging – an inexpensive and convenient alternative to functional MRI. In the UH3 phase, we propose to follow-up participants at age 18 and 21 to assess the effects of in utero and early childhood exposure to agricultural pesticides on: 1) neurobehavioral endpoints; 2) respiratory symptoms and function; and 3) body mass and related metabolic and cardiovascular conditions. This valuable cohort has the potential to add rich data, validated protocols, and practical experience to the ECHO consortium, while also answering vital questions about the long-term sequelae of prenatal and early life exposure to pesticides on adult health.

抽象的 萨利纳斯母亲和儿童健康评估中心 (CHAMACOS) 是一个纵向 大约 600 名出生队列，主要是生活在萨利纳斯农业区的第一代墨西哥裔美国儿童 加利福尼亚州山谷。 CHAMACOS 的成立与 ECHO 的目的相同：研究早期的协会 生活环境暴露与出生结果、神经发育、呼吸系统健康和肥胖有关。 我们有 跟踪儿童从子宫内到青春期，现在我们建议跟踪他们直至成年。 在过去的 17 年里，我们制定了有效的策略来注册、跟踪和保留学习 低收入拉丁裔人口的参与者；我们已经成功收集了所有四个方面的高质量数据 ECHO 的焦点终点；我们收集、处理、运输和存储了超过 150,000 个不同的 生物和环境样品；我们测量了 100 多种化学品暴露情况；我们提供了帮助 开发和测试复杂和新颖的暴露、遗传和表观遗传实验室测定；我们有 建立了牢固的社区与大学合作伙伴关系，赢得了参与者和社区的信任 利益相关者。因此，CHAMACOS 可以作为 ECHO 的模型出生队列，其长度和广度都是罕见的 对儿童从出生到成年的评估， 提供有价值的方法、经验教训和 更大的 ECHO 联盟的机会。此外，CHAMACOS 还做出了贡献 ECHO 是一个选定的、高风险的 农场工人社区中的拉丁裔儿童人口 。由于该群体中有 60% 的人超重或肥胖，我们还 提供有关代谢、心血管和肝脏疾病高风险儿童的数据。 因为 这 查马科斯 孩子们已接近成年，我们 具有独特的优势来检查成人疾病的早期起源 并解决早期生命暴露与多个健康终点之间关系的重要差距。 我们曾报道，子宫内接触有机磷农药 (OP) 与较差的健康状况相关。 学龄期注意力和执行功能、智商较低、呼吸道症状和肺功能较差。在 在 UG3 阶段，我们的目标是测试新方法，以扩大我们对这些关系的理解， 为 ECHO 贡献尖端方法。我们建议：1）检查新研究的表观遗传学 修饰，羟甲基胞嘧啶（5-hmC）——理解分子的潜在强有力的候选者 对神经发育的潜在影响机制； 2) 应用近红外光谱 (fNIR) 功能性神经影像——功能性 MRI 的一种廉价且方便的替代方案。在UH3阶段，我们 建议对 18 岁和 21 岁的参与者进行随访，以评估子宫内和幼儿期的影响 接触农业农药会影响：1) 神经行为终点； 2）呼吸道症状和功能； 3) 体重以及相关的代谢和心血管状况。这个有价值的群体有潜力 为 ECHO 联盟添加丰富的数据、经过验证的协议和实践经验，同时还回答 关于产前和生命早期接触农药对成人健康造成的长期后遗症的重要问题。