National Swine Resource and Research Center 1

摘要(总体) 本申请的总体目标是继续和扩大国家生猪资源和 研究中心(NSRRC)。NSRRC已经运行了第14个年头，它已经开发了 需要基础设施来协助跨多个学科的生猪研究，包括 异种移植。NSRRC通过基因工程开发了新的人类疾病模型，它 它招募了其他人创建的模型，并将专业知识、细胞、组织、器官和猪分发给 美国各地的调查人员。由于猪的基因工程需要专门的设备 和专业知识，NSRRC通过使用最新的 基因编辑技术创造新的基因工程猪模型。NSRRC还提供 通过与个人互动、出版手稿和赞助研讨会进行培训和教育 以及促进使用猪来研究人类疾病的会议。NSRRC拥有最先进的 具有高生物安全性的建筑，可饲养无特定病原体的动物。该计划的具体目标 下一批资助期如下： 1.运营国家生猪研究资源中心。NSRRC的职能将继续 包括：a)进口人类健康和疾病的现有猪模型；b)将猪重新衍生为 消除特定病原体，并进行健康监测，以确保保持特定病原体不存在 C)配子、胚胎和体细胞的超低温保存和储存，以防止将来丢失 有价值的模型；d)专业知识、试剂、细胞、组织、器官和无病原体猪的分布；e) 使用最先进的技术创造生物医学所需的新的转基因猪 研究社区。严格的质量控制将确保只分发高质量的基因。这个 NSRRC还将作为与使用猪的研究相关的培训和教育活动的场所 模特们。 2.开展有利于NSRRC和生物医学研究界的创新研究。 应用研究项目的目的是改进：a)猪生殖细胞的冷冻保存和 组织，b)生产转基因猪的方法，c)最先进的检测方法 猪的健康监测，以及d)适当的猪的大小，以便更多地采用猪模型。

Abstract (Overall) The overall goal of this application is to continue and expand operation of the National Swine Resource and Research Center (NSRRC). The NSRRC is in its 14th year of operation and it has developed the infrastructure needed to assist swine-based research across multiple disciplines, including xenotransplantation. The NSRRC has developed new models of human disease by genetic engineering, it has recruited models created by others, and it has distributed expertise, cells, tissues, organs and pigs to investigators throughout the United States. Since genetic engineering of pigs requires specialized facilities and expertise, the NSRRC provides invaluable services to the research community by using the latest in gene editing technology to create new genetically-engineered swine models. The NSRRC also provides training and education by interacting with individuals, publishing manuscripts and sponsoring workshops and meetings that promote the use of pigs for studying human diseases. The NSRRC has a state-of-the-art building with high biosecurity to house animals free of specific pathogens. The Specific Aims for the upcoming grant period are as follows: 1. To operate the National Swine Research and Resource Center. Functions of the NSRRC will continue to include: a) importation of existing swine models of human health and disease; b) rederivation of pigs to eliminate specific pathogens, and health monitoring to assure maintenance of a specific pathogen-free status; c) cryopreservation and storage of gametes, embryos, and somatic cells to prevent future loss of valuable models; d) distribution of expertise, reagents, cells, tissues, organs, and pathogen-free pigs; and e) use state of the art technology for the creation of new genetically-engineered pigs needed by the biomedical research community. Rigorous quality control will ensure that only high quality genetics are distributed. The NSRRC will also serve as a site for training and educational activities related to research employing swine models. 2. To perform innovative research that will benefit the NSRRC and the biomedical research community. Applied research projects are aimed at improving: a) the cryopreservation of pig reproductive cells and tissues, b) methods for the production of genetically-engineered pigs, c) state-of-the-art testing methods for swine health surveillance, and d) right-size the pig for greater adoption of pig models.