National Swine Resource and Research Center

描述(由申请者提供)：本申请的总体目标是继续运营国家猪资源研究中心(NSRRC)。到目前为止，NSRRC才刚刚开始运作的第四个年头，它已经成功地开发了必要的基础设施，以帮助跨多个学科的猪为基础的研究。在功能上，NSRRC一直积极参与招募和将猪模型引入该中心，并将细胞、组织、器官和猪分发给全美各地的调查人员。NSRRC还通过创建研究人员所需的新的转基因猪模型以及培训和教育科学家在生物医学研究中使用猪来为研究界提供宝贵的服务。重要的是，我们已经建造了一座全新的国家养猪中心大楼，具有最先进的高度生物安全，以容纳NSRRC的所有功能。下一批赠款的具体目标如下： 具体目标： 1.运营国家生猪研究资源中心。NSRRC的职能将继续包括：(A)引进关于人类健康和疾病的现有猪模型；(B)重新衍生猪以消除病原体，并进行健康监测，以确保保持无病原体状态；(C)配子、胚胎和体细胞的超低温保存和储存，以防止未来失去宝贵的模型；(D)分发试剂、细胞、组织、器官和指定的无病原体猪；以及(E)创造生物医学研究界所需的新的转基因猪。 2.开展有利于NSRRC和生物医学研究界的创新研究。研究项目旨在改进：a)猪生殖细胞和组织的冷冻保存；b)开发用于猪健康监测的最先进的血清学测试方法；以及c)开发改进的转基因猪的生产方法。NSRRC还将作为与使用猪模型的研究相关的培训和教育活动的场所。

DESCRIPTION (provided by applicant): The overall goal of this application is to continue operation of the National Swine Resource and Research Center (NSRRC). To date, the NSRRC is just beginning its fourth year of operation and it has successfully developed the infrastructure needed to assist swine-based research across multiple disciplines. Functionally the NSRRC has been actively engaged in recruiting and importing swine models into the Center and in distributing cells, tissues, organs and pigs to investigators throughout the United States. The NSRRC also provides invaluable services to the research community by creating new genetically-modified swine models needed by investigators and by training and educating scientists regarding the use of swine in biomedical research. Importantly, we have built an entirely new National Swine Center building, with state-of-the-art high biosecurity, to house all of the functions of the NSRRC. The Specific Aims for the upcoming grant period are as follows: Specific Aims: 1. To operate the National Swine Research and Resource Center. Functions of the NSRRC will continue to include: (a) importation of existing swine models of human health and disease, (b) rederivation of pigs to eliminate pathogens and health monitoring to assure maintenance of a pathogen-free status, (c) cryopreservation and storage of gametes, embryos and somatic cells to prevent future loss of valuable models, (d) distribution of reagents, cells, tissues, organs and designated pathogen-free pigs, and (e) creation of new genetically-modified pigs needed by the biomedical research community. 2. To perform innovative research that will benefit the NSRRC and the biomedical research community. Research projects are aimed at improving: a) the cryopreservation of pig reproductive cells and tissues; b) developing state-of-the-art serologic testing methods for swine health surveillance; and c) the development of improved methods for the production of genetically-modified pigs. The NSRRC will also serve as a site for training and educational activities related to research employing swine models.