Multi-modal AO-LSO Phase Gradient Imaging of the Inner Retina

内视网膜多模态 AO-LSO 相位梯度成像

• 批准号: 9788095
• 负责人: Mircea Mujat
• 金额: $ 51.09万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9788095
• 关键词: Address; Algorithms; Anatomy; Back; Biological Markers; Blood flow; Burn injury; Cells; Clinical; Clinical Research; Collaborations; Collection; Data; Detection; Development; Diabetic Retinopathy; Diagnostic; Disease; Documentation; Ear; Early identification; Engineering; Evaluation; Eye; Future; General Practices; Glaucoma; Government; Human; Image; Imaging technology; Inflammatory; Lighting; Medical center; Modality; Nerve Degeneration; Nerve Fibers; Neurodegenerative Disorders; Neurons; New York; Ophthalmology; Ophthalmoscopes; Ophthalmoscopy; Optics; Pathology; Patients; Performance; Pericytes; Persons; Phase; Photophobia; Photoreceptors; Process; Research; Research Personnel; Resolution; Resources; Retina; Retinal; Retinal Diseases; Retinal Ganglion Cells; Sales; Scanning; Site; Speed; Structure; System; Testing; Time; Tissues; United States National Institutes of Health; Work; adaptive optics; adaptive optics scanning laser ophthalmoscopy; base; biomarker identification; clinical application; clinical care; clinical imaging; confocal imaging; design; experience; gene therapy; high resolution imaging; imager; imaging approach; imaging capabilities; imaging platform; in vivo; insight; instrument; multimodality; neuronal cell body; neurovascular; next generation; novel; novel therapeutics; phase 1 testing; physical science; preservation; programs; prototype; receptor; relating to nervous system; research clinical testing; retinal imaging; retinal neuron; success; systems research; time use; tool; transmission process

Project Summary/Abstract Physical Sciences Inc. (PSI) is continuing the pursuit of new clinical applications and advanced multimodal capabilities for its novel retinal imaging technology based on an adaptive optics line-scanning ophthalmoscope (AO-LSO). The Compact Adaptive Optics Retinal Imager (CAORI) eliminates high-speed scanning components, reduces the clinical footprint compared to research adaptive optics scanning laser ophthalmoscopes (AOSLO), and simplifies AO optical design while preserving the confocal advantage. In the proposed Phase II program, PSI will incorporate a powerful new modality for phase gradient imaging (PGI) in the inner retina. CAORI originally emphasized line-confocal imaging of photoreceptors in the outer retina in the bright-field reflectance mode. However, in recent years, following the groundbreaking work of AOSLO researchers, so-called “dark-field” and split-detection AOSLO modalities with various combinations of offset detection apertures allow very subtle phase objects to be indirectly imaged in relatively transparent retinal layers, including microvasculature and neural somas (cell bodies of retinal ganglion cells, for example). Such features can used for early identification of new biomarkers in neurovascular and neurodegenerative conditions such as diabetic retinopathy and glaucoma. In Phase I, PSI has proven that CAORI can be adapted, within the same general line-confocal paradigm and footprint, for direct, sensitive, multi-aperture phase gradient imaging (PGI) in the inner retina. In particular, a time domain integration (TDI) line-camera enables line-confocality of the AO-LSO to be broadly adjusted, increasing collection aperture(s) without loss of image resolution (~2.4µm) or light sensitivity. Simultaneous bright-field confocal ophthalmoscopy and a new type of oblique back- illuminated line ophthalmoscopy for directly imaging inner retinal layers in transmission was demonstrated on a single focal plane. Differencing pairs of inner retinal-focused TDI images (and videos) with complementary line offsets produces phase gradient images—the line-field AO-LSO equivalent of split-detection AOSLO. PSI will modify existing CAORI beta prototypes for PGI and begin clinical testing of these system in collaboration with researchers at New York Eye and Ear Infirmary, Mount Sinai (diabetic retinopathy), and NYU Langone Medical Center (glaucoma).

项目总结/摘要 物理科学公司(PSI)正在继续追求新的临床应用和先进的多模式 基于自适应光学线扫描检眼镜的新型视网膜成像技术 （AO-LSO）。紧凑型自适应光学视网膜成像仪（CAORI）消除了高速扫描 与研究自适应光学扫描激光器相比， 这是一种新型的光学系统，它与普通检眼镜（AOSLO）相比，简化了AO光学设计，同时保留了共焦优势。在 在拟议的第二阶段计划中，PSI将采用一种功能强大的相位梯度成像（PGI）新模式， 内层视网膜CAORI最初强调在视网膜外层的光感受器的线共聚焦成像， 明场反射模式。然而，近年来，继AOSLO的开创性工作 研究人员，所谓的“暗场”和具有各种偏移组合的分离检测AOSLO模式 检测孔径允许非常细微的相位物体在相对透明的视网膜中间接成像 层，包括微血管和神经胞体（例如视网膜神经节细胞的细胞体）。等 特征可用于神经血管和神经退行性疾病中新生物标志物的早期鉴定 如糖尿病视网膜病变和青光眼。在第一阶段，PSI已经证明，CAORI可以适应， 相同的一般线共焦范例和足迹，用于直接、灵敏、多孔径相位梯度成像 (PGI)在视网膜内层特别地，时域积分（TDI）线相机使得能够实现以下的线共焦性： AO-LSO将被广泛调整，增加收集孔径而不损失图像分辨率（~2.4 µ m） 或光敏感性。同时明场共焦检眼镜和一种新型的斜背- 照明线检眼镜直接成像视网膜内层的传输证明了一个 单焦平面利用互补线区分视网膜内聚焦TDI图像（和视频）对 偏移产生相位梯度图像-线场AO-LSO相当于分裂检测AOSLO。PSI将 修改现有的CAORI β原型，用于PGI，并与合作伙伴开始这些系统的临床测试 纽约眼耳医院、西奈山（糖尿病视网膜病变）和纽约大学朗格尼医学中心的研究人员 中心（青光眼）。