Multi-modal AO-LSO Phase Gradient Imaging of the Inner Retina

项目摘要/摘要 物理科学公司(PSI)正在继续追求新的临床应用和先进的多模式 其基于自适应光学行扫描检眼镜的新型视网膜成像技术的能力 (AO-LSO)。紧凑型自适应光学视网膜成像仪(CAORI)消除了高速扫描 组件，与研究自适应光学扫描激光器相比，减少了临床足迹 AOSLO)，简化了声光光学设计，同时保留了共焦优势。在 提出的第二阶段计划，PSI将纳入一种强大的新模式的相位梯度成像(PGI)在 内层视网膜。CAORI最初强调视网膜外部感光细胞的线共聚焦成像。 明场反射模式。然而，近年来，在AOSLO的开创性工作之后 研究人员，具有不同偏移量组合的所谓“暗视野”和分裂探测AOSLO模式 探测孔允许非常细微的相位物体在相对透明的视网膜中间接成像 层，包括微血管和神经体(例如，视网膜神经节细胞的细胞体)。是这样的 特征可用于早期识别神经血管和神经退行性疾病的新生物标志物 如糖尿病视网膜病变和青光眼。在第一阶段，PSI已经证明了CAORI可以在 对于直接、灵敏、多孔径的相位梯度成像，相同的通用线共焦范例和足迹 (PGI)在视网膜内。具体地说，时间域积分(TDI)线摄像机能够实现 AO-LSO将进行广泛调整，在不损失图像分辨率(~2.4µm)的情况下增加采集孔径(S) 或者光敏感度。同步明视场共焦检眼镜和一种新型斜背式眼底镜 照明的线检眼镜直接成像在传输中的视网膜内层。 单焦平面。用互补线条区分对内视网膜聚焦的TDI图像(和视频) 偏移量产生相位梯度图像--相当于分裂检测AOSLO的线场AO-LSO。PSI将 修改用于PGI的现有CAORI测试版原型，并与合作开始这些系统的临床测试 纽约眼耳医院、西奈山(糖尿病视网膜病变)和纽约大学朗格尼医学中心的研究人员 中心(青光眼)。

Project Summary/Abstract Physical Sciences Inc. (PSI) is continuing the pursuit of new clinical applications and advanced multimodal capabilities for its novel retinal imaging technology based on an adaptive optics line-scanning ophthalmoscope (AO-LSO). The Compact Adaptive Optics Retinal Imager (CAORI) eliminates high-speed scanning components, reduces the clinical footprint compared to research adaptive optics scanning laser ophthalmoscopes (AOSLO), and simplifies AO optical design while preserving the confocal advantage. In the proposed Phase II program, PSI will incorporate a powerful new modality for phase gradient imaging (PGI) in the inner retina. CAORI originally emphasized line-confocal imaging of photoreceptors in the outer retina in the bright-field reflectance mode. However, in recent years, following the groundbreaking work of AOSLO researchers, so-called “dark-field” and split-detection AOSLO modalities with various combinations of offset detection apertures allow very subtle phase objects to be indirectly imaged in relatively transparent retinal layers, including microvasculature and neural somas (cell bodies of retinal ganglion cells, for example). Such features can used for early identification of new biomarkers in neurovascular and neurodegenerative conditions such as diabetic retinopathy and glaucoma. In Phase I, PSI has proven that CAORI can be adapted, within the same general line-confocal paradigm and footprint, for direct, sensitive, multi-aperture phase gradient imaging (PGI) in the inner retina. In particular, a time domain integration (TDI) line-camera enables line-confocality of the AO-LSO to be broadly adjusted, increasing collection aperture(s) without loss of image resolution (~2.4µm) or light sensitivity. Simultaneous bright-field confocal ophthalmoscopy and a new type of oblique back- illuminated line ophthalmoscopy for directly imaging inner retinal layers in transmission was demonstrated on a single focal plane. Differencing pairs of inner retinal-focused TDI images (and videos) with complementary line offsets produces phase gradient images—the line-field AO-LSO equivalent of split-detection AOSLO. PSI will modify existing CAORI beta prototypes for PGI and begin clinical testing of these system in collaboration with researchers at New York Eye and Ear Infirmary, Mount Sinai (diabetic retinopathy), and NYU Langone Medical Center (glaucoma).