Hyperspectral Optical Tomography for Molecular Imaging

用于分子成像的高光谱光学断层扫描

基本信息

批准号：
7416788
负责人：
Simon R Cherry
金额：
$ 27.89万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-05-18 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7416788
关键词：
Address Algorithms Anatomic Models Anatomy Animal Experiments Animal Model Animals Atlases Bayesian Method Beds Caliber Cherry - dietary Collection Complex Computer software Computers Computing Methodologies Contrast Media Coupled Data Data Collection Dependence Depth Detection Development Diffusion Dimensions Drug Formulations Elements Equation Evaluation Fatty acid glycerol esters Film Fluorescence Fluorescent Probes Gene Delivery Goals Gold Head Image Injection of therapeutic agent Ink Life Light Lighting Localized Location Lung Malignant neoplasm of prostate Mammary gland Maps Measurement Measures Methodology Methods Metric Microspheres Mind Modality Modeling Mus Neoplasm Metastasis Noise Numbers Optical Tomography Optics Organ Pattern Physics Play Positron Probability Process Production Property Proteins Range Relative (related person)Reporter Research Research Personnel Resolution Rodent Model Role Series Shapes Signal Transduction Simulate Solutions Source Spatial Distribution Speed Standards of Weights and Measures Statistical Models Structure Surface System Systems Analysis Testing Three-Dimensional Image Tissues Translations Validation X-Ray Computed Tomography absorption base cost density design detector fluorescence imaging fluorophore human disease image reconstruction in vivo information gathering instrumentation interest liquid crystal molecular imaging mouse model optical imaging optical sensor preconditioning programs prototype reconstruction research study response simulation three-dimensional modeling validation studies vector

项目摘要

In vivo fluorescence imaging, utilizing fluorescent reporter proteins, or exogenously administered fluorescent probes, plays an important role in the interrogation of biologic systems, particularly the study of rodent models of human disease. The majority of systems to date produce 2-D images of the integrated light distribution emitted at the surface of the animal, severely compromising the ability to quantify and accurately localize signals due to the strong depth dependence of the optical signal. There is great interest in extending in vivo fluorescence imaging methodology to the reconstruction of volumetric images that can show the location and concentration of fluorescent proteins or fluorescent contrast agents in living mice. This would be significant, as it would enable quantitative molecular imaging with fluorescent approaches at considerably less cost and potentially higher spatial and temporal resolution than competing modalities such as positron emissiontomography. Incorporating spectral information into the reconstructionprocess provides additional information about source depth due to the dependence of tissue absorption and scattering properties on wavelength. Spectral information therefore has the potential to increase depth resolution of fluorescence reconstruction as compared to reconstruction from traditional monochromatic or broadband measurements. Furthermore, by using a whole camera image instead of a small number of optical detectors for reconstruction, the amount of information gathered from the surface of the animal is increased and should also contribute to enhanced fluorophore reconstructions. For the proposed project, we plan to develop a hyperspectral optical imaging system and algorithms, which are capable of quantitative three-dimensional tomographic reconstructions of fluorophore distributions in small animals. In order to achieve this goal, a number of critical research issues will be addressed, including system design, optimization of excitation and detection geometries, efficient and accurate modeling of light transport in the mouse and efficient image reconstruction strategies. A series of simulations, phantom and animal experiments will be used to validate the approach and the results will be compared to standard broadband methods that utilize high-pass filters.

体内荧光成像，利用荧光报告蛋白或外源给药荧光探针在生物系统的审问中起着重要作用，尤其是研究人类疾病的啮齿动物模型。迄今为止，大多数系统都会产生集成光的2-D图像分布在动物的表面发射，严重损害了量化和准确的能力由于光学信号的深度依赖性而导致的定位信号。对扩展非常感兴趣体内荧光成像方法，以重建体积图像，可以显示活小鼠中荧光蛋白或荧光对比剂的位置和浓度。这会要重要，因为它可以通过相当多的荧光方法实现定量分子成像比竞争方式（例如正电子）的成本更低，空间和时间分辨率更高发射术学。将光谱信息纳入重建程序提供了有关的其他信息由于组织吸收和散射特性对波长的依赖性引起的源深度。光谱因此，信息有可能增加荧光重建的深度分辨率与传统的单色或宽带测量值重建相比。此外，由使用整个相机图像，而不是少量的光学检测器进行重建，从动物表面收集的信息增加了，也应有助于增强荧光团重建。对于拟议的项目，我们计划开发高光谱光学成像系统和算法，能够对荧光团分布进行定量的三维层析成像重建在小动物中。为了实现这一目标，将解决许多关键的研究问题，包括系统设计，优化激发和检测几何形状，高效且准确的建模小鼠中的光传输和有效的图像重建策略。一系列模拟，幻影动物实验将用于验证方法，并将结果与标准进行比较利用高通滤波器的宽带方法。