Mid-Region AIDS and Cancer Specimen Resource Consortium

描述（由申请人提供）：美国国家癌症研究所（NCI）艾滋病研究计划的艾滋病和癌症标本资源（ACSR）为资助/批准的癌症研究人员提供艾滋病毒/艾滋病相关的恶性组织和数据，以促进对艾滋病毒感染和某些癌症之间关系的全球理解。中部地区ACSR（MR-ACSR）联盟，包括俄亥俄州州立大学（OSU）、埃默里大学、拉什大学、田纳西大学、孟菲斯以及乌干达和肯尼亚的东非分支机构，建议（具体目标1）维持一个中区ACSR（MR-ACSR）符合所有ACSR规则和建议（包括NCI生物标本资源最佳实践和适用的符合联邦法规的要求，并设有运营办公室，以执行收集、增强、储存和分发HIV相关、质量有保证的人类肿瘤组织以及相关临床、病理学、视觉和人口统计学数据给经批准的研究者的主要任务。为了确保最佳利用这些资源，MR-ACSR建议（具体目标2）与ACSR区域生物储存库、NCI艾滋病生物储存库计划（AMP）和其他NCI艾滋病相关恶性肿瘤和癌症生物储存库计划以及俄亥俄州州立大学病理学系人类组织资源网络（HTRN）合作，以利用高质量标本和数据可用性，在艾滋病毒/艾滋病相关恶性肿瘤领域进行转化研究。为了实现NCI促进健康的总体目标，MR-ACSR必须（具体目标3）开发原创和创新计划，利用MR-ACSR联盟（包括东非附属机构）的实验室设施和样本资源，提高生物样本和相关数据的质量、可用性和相关性，俄亥俄州州立大学病理学系的HTRN和生物医学信息学系的caBIG项目。MR-ACSR提出了一种新的组织固定方法的部署，以改善研究组织的RNA，DNA和组织蛋白的保存。组织微阵列（TMA）的建设可以部署使用“现场”的建设格式，有利于诊断标本收集在资源有限的位置。我们建议使用这种TMA格式与后续的远程处理和免疫组化染色，以促进撒哈拉以南非洲淋巴瘤（NHL）的组织表型。MR-ACSR还有一个支持数字显微镜设施，用于远程可视化互联网咨询，使用组织的数字化图像与我们在东非的合作者共享数据，特别是与癌症临床治疗试验中的病理组织诊断咨询和审查有关。最后，为了充分利用可视化数据和远程协作，MR-ACSR建议与caGrid的开发人员合作，将其作为数字图像数据的临床资源。

DESCRIPTION (provided by applicant): The AIDS and Cancer Specimen Resource (ACSR) of the National Cancer Institutes (NCI) AIDS Malignancy Program provides HIV/AIDS-related malignant tissues and data to funded/approved cancer researchers to promote a global understanding of the relationships between HIV infection and certain cancers. The Mid Region ACSR (MR-ACSR) consortium, including The Ohio State University (OSU), Emory University, Rush University, University of Tennessee, Memphis, and East African affiliates in Uganda and Kenya, proposes to (specific aim 1) maintain a mid-region ACSR (MR-ACSR) biorepository with affiliates that is compliant with all ACSR rules and recommendations including the NCI Best Practices for Biospecimen Resources and applicable federal regulations and is adequately structured with an Operations Office to perform the principal tasks of collecting, enhancing, storing, and distributing HIV-related, quality assured human tumor tissue with associated clinical, pathological, visual and demographic data to approved investigators. To assure best use of these resources, the MR-ACSR proposes to (specific aim 2) collaborate with ACSR regional biorepositories, NCI AIDS Malignancy Program (AMP) and other NCI AIDS related malignancy and cancer biorepository programs and The Ohio State University Department of Pathology's Human Tissue Resource Network (HTRN) to leverage quality specimen and data availability for translational research in the area of HIV/AIDS-related malignancies. To accomplish the overall objectives of the NCI to promote good health, the MR-ACSR must (specific aim 3) develop original and innovative programs to improve the quality, availability and relevance of biospecimens and associated data using the laboratory facilities and specimen resources of the MR-ACSR consortium including the East African affiliates, The Ohio State University Department of Pathology's HTRN and the Department of Biomedical Informatics caBIG project. MR-ACSR proposes the deployment of a new tissue fixation method to improve preservation of RNA, DNA and tissue proteins for research tissues. Tissue microarray (TMA) construction can be deployed using a "field" construction format that facilitates diagnostic specimen collection in resource constrained locations. We propose to facilitate the tissue phenotyping of lymphoma (NHL) in sub-Saharan Africa using this TMA format with follow up distant processing and immunohistochemical staining. The MR-ACSR also has a supporting digital microscopy facility for remote visual Internet consultation using digitized images of tissue for data sharing with our collaborators in East Africa, particularly related to diagnostic consultation and review of pathology tissues in cancer clinical treatment trials. Finally, to make the best use of visual data and distant collaboration, the MR-ACSR proposes to work with the developers of caGrid as a clinical resource for digital image data.