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Antimicrobial Resistant Bacteria: Exposures and Health of Cattle Workers

耐药细菌：养牛工人的接触和健康

基本信息

批准号：
10783823
负责人：
Sheryl Magzamen
金额：
$ 60.8万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10783823
关键词：
Antimicrobial Resistant Bacteria Exposures Health

项目摘要

Abstract Livestock workers are at the frontline of exposure to agriculture-generated bioaerosols containing a diverse mixture of respiratory pathogens. The misuse and overuse of antibiotics in agriculture has been posited as a potential driver in the accelerated development of antibiotic resistant genes (ARG). Animal handling, waste management, and bioaerosol generation may contribute to relatively high personal exposure to antibiotic resistant bacteria (ARB) on farms. The short- and long-term health effects of these bacteria are currently unknown. Moreover, workers may act as a vector – disseminating ARG and viruses. Specifically, cattle workers are at greater risk of Influenza D virus, an emerging pathogen associated with acute respiratory disease. The workers may also pose a threat to the animals, such as introducing strains of multi-drug resistant tuberculosis (TB). We propose to address critical questions that remain about the impacts of worker exposure to respiratory pathogens and transmission to animals, farms and the larger community. The eighteen year collaborative research program conducted by the High Plains Intermountain Center for Agricultural Health and Safety and regional dairy and beef producers offers a unique opportunity to investigate the role of workplace exposure on human-hosted bacterial communities (microbiome), associated antibiotic resistance genes (ARG, or resistome) and respiratory viruses (virome) and respiratory health in cattle workers. The research objective is to ascertain if ARG and viruses are underlying risk factors for subclinical markers of respiratory inflammation. The central hypothesis is that the nasal microbiome of cattle workers will have distinct microbial communities and genetic constitution that are associated with measurable levels of inflammation and airway resistance as compared to occupational controls and housemates. The specific aims are to: (1) compare and contrast the composition of the microbiome, resistome, and virome among dairy and beef workers; (2) model exposure-response relationships in the context of microbiome, resistome, virome and inflammatory markers and airway resistance among dairy and beef workers; and (3) ascertain subclinical health impacts of shared microbiome, resistome, and virome between occupational and household settings. This project unites scientists with expertise in occupational health and exposure science, epidemiology, microbiology, genetics, and bioinformatics. This study will contribute to greater awareness of workplace exposures and transmission of ARG and emerging viruses among dairy and beef workers.

摘要畜牧业工人处于暴露于农业产生的生物气溶胶的第一线，混合呼吸道病原体。在农业中滥用和过度使用抗生素被认为是一种抗生素耐药基因（ARG）加速发展的潜在驱动因素。动物处理，废物管理和生物气溶胶的产生可能导致相对较高的抗生素个人暴露耐药细菌（ARB）在农场。这些细菌的短期和长期健康影响目前未知此外，工蜂可能作为传播ARG和病毒的媒介。特别是养牛工人 D型流感病毒是一种与急性呼吸道疾病有关的新出现的病原体。的工人也可能对动物构成威胁，例如引入耐多药结核病菌株（TB）。我们建议解决关键问题仍然是关于工人暴露于呼吸系统的影响，病原体和传播给动物，农场和更大的社区。十八年合作高平原山间农业健康与安全中心进行的研究计划，区域奶制品和牛肉生产商提供了一个独特的机会，调查工作场所暴露的作用，人类宿主细菌群落（微生物组），相关抗生素耐药基因（ARG或耐药基因组）和呼吸道病毒（病毒组）和牛工人的呼吸道健康。研究目的是确定 ARG和病毒是否是呼吸道炎症亚临床标志物的潜在危险因素。中央一种假设是，牛工人的鼻微生物组将具有不同的微生物群落和遗传特征。与可测量的炎症和气道阻力水平相关的体质，职业控制和室友。具体目的是：（1）比较和对比奶制品和牛肉工人中的微生物组、抗性组和病毒组;（2）模型确定-响应微生物组、耐药组、病毒组和炎症标志物与气道阻力的关系在乳制品和牛肉工人中;以及（3）确定共享微生物组，耐药基因组，和病毒组之间的关系。该项目将具有以下专业知识的科学家联合起来：职业健康和接触科学、流行病学、微生物学、遗传学和生物信息学。本研究将有助于提高对工作场所暴露以及ARG和新出现病毒传播的认识在奶制品和牛肉工人中。