Mass Spec Resource

质谱资源

• 批准号: 7645376
• 负责人: STEPHEN B. CALDERWOOD
• 金额: $ 17.75万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7645376
• 关键词: Applied Research; Charge; Communication; Development; Doctor of Medicine; Isotope Labeling; Mails; Microbe; Mission; National Institute of Allergy and Infectious Disease; New England; Peptides; Proteins; Research Personnel; Resolution; Resources; Scanning; Work; antimicrobial; inorganic phosphate; instrument; mass spectrometer; pathogen

NERCE: Mass Spec Resource - Stephen Calderwood, M.D. The LTQ XL is a fast scanning tandem mass spectrometer, capable of sequencing several peptides every second, allowing rapid characterization and quantification (using isotope labels) of proteins from microbes. The instrument also has several intermediate scan modes, allowing for neutral loss analysis of phosphate groups, as well as some medium full scan resolution modes for charge state assessment. The instrument has a higher duty cycle than the LTQ-FT, and a higher sensitivity for lower level peptides. Use of this instrument is prioritized for New England investigators working on NIAID Priority Pathogens, for which rapid identification of proteins and peptides will facilitate basic and applied science of relevant to the missions of NERCE, such as the identification of pathogen proteins that are potential targets for antimicrobial development. This resource is publicized to NERCE investigators via e-mail, the NERCE Website, and direct communication when appropriate.

NERCE：质谱资源- Stephen Calderwood，医学博士。 LTQ XL是一种快速扫描串联质谱仪，能够对多种肽进行测序 每秒钟，允许快速表征和定量（使用同位素标记）蛋白质， 微生物该仪器还具有几种中间扫描模式，允许中性损耗分析， 磷酸基团，以及一些中等全扫描分辨率模式，用于电荷状态评估。的 仪器具有比LTQ-FT更高的占空比，并且对于较低水平的肽具有更高的灵敏度。 新英格兰研究NIAID优先病原体的研究人员优先使用该仪器， 蛋白质和肽的快速鉴定将促进相关的基础和应用科学， NERCE的任务，例如鉴定病原体蛋白质，这些蛋白质是 抗菌剂的开发该资源通过电子邮件向NERCE调查人员公布， 网站，并在适当的时候直接沟通。