Enhanced Protein Separations Using Molecular Micelles

使用分子胶束增强蛋白质分离

• 批准号: 7487946
• 负责人: ISIAH M WARNER
• 金额: $ 25.97万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-20 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7487946
• 关键词: Area; Arterial Fatty Streak; Arteries; Biological Markers; Biomedical Research; Blood capillaries; Bypass; Charge; Chemical Engineering; Chemistry; Class; Collaborations; Complex Mixtures; Coupled; Data; Detection; Development; Devices; Film; Funding; Glycoproteins; Goals; Grant; Institutes; Laboratories; Lysine; Malignant Neoplasms; Measurement; Methods; Micelles; Microfluidic Microchips; Microfluidics; Molecular; Phase; Plague; Polymers; Procedures; Proteins; Public Health; Reagent; Research; Research Personnel; Sampling; Solutions; Standards of Weights and Measures; Structure; Technology; Tubular formation; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Work; base; capillary; concept; desire; gel electrophoresis; improved; interest; mass spectrometer; molecular recognition; nanolitre; nanolitre scale; novel; success; tool

DESCRIPTION (provided by applicant): While based on studies performed during the previous NIH grant period, this proposal outlines research which focuses in a fundamentally different direction. The overall goal of the research outlined in this proposal is to explore enhanced methods for separation of small protein samples. Three focus areas are outlined to achieve this overall goal. The first focus area is the primary area of research and involves development of open-tubular capillary electrochromatography (OT-CEC) for separation of microliter quantities of proteins. In this first area, very robust polyelectrolyte multilayer (PEM) coatings generated from alternate rinses of cationic and anionic polymers will be used in the OT-CEC mode. The ionic polymers which will be initially used in this phase of our research will be based on molecular micelles similar to those developed during our previous funding period. Other anionic polymers will be added as the research develops. The cationic polymers will also be polymers (e.g. poly-lysine) similar to those used during our previous funding period. However, new cationic molecular micelles will also be synthesized and explored in combination with our anionic molecular micelles as new forms of PEM coatings. In addition, we will explore the utility of zwitterionic polymers for PEM coatings in OT-CEC. A critical component of this research is to understand the structure of our PEM coatings. Characterization of our PEM coatings will be done in collaboration with Dr. Paula Hammond of the department of chemical engineering of MIT. The second focus area of this proposed research is to explore the applicability of the OT-CEC technology developed during the first phase to microfluidic platforms for analyses of nanoliter volumes of proteins. This research will be done in collaboration with Dr. Laurie Locascio of the National Institute of Standards and Technology. The third phase of this research involves the use of molecular micelles to improve the separation of proteins using gel electrophoresis. As with the other focus areas, various strategies will be exploited to optimize separations for different classes of proteins. Finally, it is noted that this project is relevant to almost all areas of biomedical research since proteins are ubiquitous. Thus, isolation, detection, and identification of small quantities of proteins as outlined in this proposal are important to almost all aspects of public health.

描述（由申请人提供）：虽然基于在上一个NIH赠款期间进行的研究，但该提案概述了侧重于根本不同方向的研究。该提案中概述的研究的总体目标是探索分离小蛋白质样品的增强方法。概述了三个焦点领域以实现这一总体目标。第一个焦点区域是研究的主要领域，涉及开发开放式毛细血管电解料（OT-CEC），用于分离微氧蛋白的分离。在第一个区域，将在OT-CEC模式下使用阳离子和阴离子聚合物的替代冲洗产生的非常健壮的聚电解质多层（PEM）涂层。最初将在我们研究的这一阶段使用的离子聚合物将基于与以前的资金期间开发的离子聚合物。随着研究的发展，将添加其他阴离子聚合物。阳离子聚合物也将是类似于我们以前的资金期间类似的聚合物（例如聚赖氨酸）。然而，新的阳离子分子胶束也将与我们的阴离子分子胶束合成并探索新形式的PEM涂层。此外，我们将探索Zwitterionic聚合物在OT-CEC中PEM涂层的效用。这项研究的一个关键组成部分是了解我们的PEM涂层的结构。我们的PEM涂料的表征将与MIT化学工程系的Paula Hammond博士合作完成。这项拟议的研究的第二个重点领域是探索第一阶段在微流体平台上开发的OT-CEC技术的适用性，以分析纳米素蛋白的量。这项研究将与国家标准技术研究所的Laurie Locascio博士合作进行。这项研究的第三阶段涉及使用分子胶束使用凝胶电泳改善蛋白质的分离。与其他重点领域一样，将利用各种策略来优化不同类别的蛋白质的分离。最后，注意到该项目与几乎所有生物医学研究领域有关，因为蛋白质无处不在。因此，该提案中概述的少量蛋白质的隔离，检测和识别对于几乎所有公共卫生的各个方面都很重要。