BIOANALYTICAL SEPARATIONS USING CHIRAL POLYMERS

使用手性聚合物进行生物分析分离

• 批准号: 6095795
• 负责人: ISIAH M WARNER
• 金额: $ 2.88万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6095795
• 关键词: analytical ultracentrifugation; capillary electrophoresis; chemical aggregate; chemical chain length; chemical synthesis; crosslink; high performance liquid chromatography; hydropathy; infrared spectrometry; interferometry; light scattering; mass spectrometry; mathematical model; micelles; molecular polarity; neutron diffraction; nuclear magnetic resonance spectroscopy; peptides; physical separation; polyglutamates; polymerization; polymers; stereoisomer; surfactant; thermodynamics

DESCRIPTION: (Adapted from the applicant's abstract) This project focuses on synthesis and development of new chiral reagents for use in chiral chromatography for resolution of racemic mixtures. Three different approaches are suggested for this research. The first involves the use of chiral micelle polymers as mobile phase additives to achieve chiral interactions in the mobile phase. The polymeric micelles are produced by photo-induced covalent linkage of the monomers at concentrations above the critical micelle concentration (CMC). These polymers have many of the features of normal micelles except that they do not have a CMC value. Thus, much of the dynamic equilibria which is associated with conventional micelles is eliminated. It is proposed that this property should enhance chiral discrimination in the mobile phase. In addition, much lower polymeric micelle concentrations can be employed compared to that required for traditional surfactant micelles enhancing chiral recognition. The second part of this proposal involves the use of linear polypeptides as stationary phases for chiral separations. It is proposed that the ability of these chiral molecules to exist in well defined chain conformations of extensive order, even in solution, should enhance chiral recognition in the stationary phase. The third component of this research involves a combination of the two approaches proposed, i.e., use of chiral micelle polymers in the mobile phase in conjunction with chiral polypeptides in the stationary phase. The application of this approach to capillary electrochromatography (CEC) opens new avenues for chiral separation.

描述：（根据申请人的摘要进行了改编）此项目重点 关于用于手性的新手性试剂的合成和开发 色谱法，用于消除外星人混合物。 三个不同 为这项研究提出了方法。 第一个涉及使用 手性胶束聚合物作为流动相添加剂以实现手性 流动阶段的交互。 聚合胶束是由 光诱导的单体以高于浓度的浓度连接 临界胶束浓度（CMC）。 这些聚合物有许多 正常胶束的特征除了它们没有CMC值。 因此， 与传统有关的大部分动态平衡 消除了胶束。 有人建议该财产应增强 手动阶段的手性歧视。 另外，要低得多 与所需的聚合物胶束浓度相比可以使用 用于传统的表面活性剂胶束，以增强手性识别。 这 该提案的第二部分涉及使用线性多肽作为 手性分离的固定相。 有人提出能力 这些手性分子存在于明确定义的链构象中 即使在解决方案中，广泛的顺序也应增强手性识别 固定阶段。 这项研究的第三部分涉及 提出的两种方法的组合，即使用手性胶束 流动阶段的聚合物与手性多肽结合 固定阶段。 这种方法在毛细管上的应用 电色谱法（CEC）为手性分离开辟了新的途径。