BIOANALYTICAL SEPARATIONS USING CHIRAL POLYMERS

使用手性聚合物进行生物分析分离

• 批准号: 6018725
• 负责人: ISIAH M WARNER
• 金额: $ 18.86万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018725
• 关键词: analytical ultracentrifugation; capillary electrophoresis; chemical aggregate; chemical chain length; chemical synthesis; crosslink; high performance liquid chromatography; hydropathy; infrared spectrometry; interferometry; light scattering; mass spectrometry; mathematical model; micelles; molecular polarity; neutron diffraction; nuclear magnetic resonance spectroscopy; peptides; physical separation; polyglutamates; polymerization; polymers; stereoisomer; surfactant; thermodynamics

DESCRIPTION: (Adapted from the applicant's abstract) This project focuses on synthesis and development of new chiral reagents for use in chiral chromatography for resolution of racemic mixtures. Three different approaches are suggested for this research. The first involves the use of chiral micelle polymers as mobile phase additives to achieve chiral interactions in the mobile phase. The polymeric micelles are produced by photo-induced covalent linkage of the monomers at concentrations above the critical micelle concentration (CMC). These polymers have many of the features of normal micelles except that they do not have a CMC value. Thus, much of the dynamic equilibria which is associated with conventional micelles is eliminated. It is proposed that this property should enhance chiral discrimination in the mobile phase. In addition, much lower polymeric micelle concentrations can be employed compared to that required for traditional surfactant micelles enhancing chiral recognition. The second part of this proposal involves the use of linear polypeptides as stationary phases for chiral separations. It is proposed that the ability of these chiral molecules to exist in well defined chain conformations of extensive order, even in solution, should enhance chiral recognition in the stationary phase. The third component of this research involves a combination of the two approaches proposed, i.e., use of chiral micelle polymers in the mobile phase in conjunction with chiral polypeptides in the stationary phase. The application of this approach to capillary electrochromatography (CEC) opens new avenues for chiral separation.

描述：(改编自申请者的摘要)本项目重点 手性用新型手性试剂的合成与发展 用于拆分外消旋混合物的层析。三种不同的 对本研究的研究方法提出了建议。第一个涉及到使用 手性胶束聚合物作为流动相添加剂实现手性 流动相中的相互作用。聚合物胶束是通过以下方式生产的 光诱导单体共价键的浓度高于 临界胶束浓度(CMC)。这些聚合物具有许多 正常胶束的特性，只是它们没有cmc值。因此， 许多动态平衡与传统的 胶束被消除。建议该属性应增强 流动相中的手性识别。此外，低得多的 可以使用与所需浓度相比较的聚合物胶束浓度 对于传统的表面活性剂胶束，增强了手性识别。这个 该提案的第二部分涉及使用线性多肽作为 用于手性分离的固定相。有人提出，这种能力 这些手性分子存在于明确定义的链状构象中 广泛的有序性，即使在溶液中，也应该增强手性识别 固定相。这项研究的第三个组成部分涉及 建议的两种方法的组合，即使用手性胶束 流动相中的聚合物与手性多肽相结合 固定相。该方法在毛细管中的应用 电色谱(CEC)为手性分离开辟了新的途径。