BIOANALYSIS--DRUG/PROTEIN INTERACTIONS

生物分析——药物/蛋白质相互作用

• 批准号: 3297087
• 负责人: ISIAH M WARNER
• 金额: $ 8.04万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297087
• 关键词: binding proteins; chemical binding; drug interactions; drug receptors; fluorescence polarization; mathematical model; nuclear magnetic resonance spectroscopy; serum albumin

The research proposed here involves bioanalytical studies of drug/protein interactions. It is proposed that a new instrumental approach to fluorescence detected dichroism (FDCD) will be used as a probe for drug/protein interactions. The FDCD instrumentation provides rapid acquisition of FDCD data and enhanced capabilities for Interpretation of optically active multicomponent fluorescence mixtures. The use of this instrumentation for selectively monitoring chiral quenching provides a powerful tool for studying protein binding of fluorescence drugs. Specifically, the overall project is divided into three areas: 1) the use of FDCD measurements to aid in understanding the binding of fluorescent drugs to human serum albumin (HSA) and select fragments of HSA; 2) the development of novel algorithms to aid in the analysis and interpretation of the acquired data and 3) the combination of FDCD measurement with other parameters of fluorescence such as quenching, depolarization and lifetimes, as well as other instrumental techniques such as IR and NMR to provide a more useful probe of the drug binding sites in selected proteins.

这里提出的研究涉及药物/蛋白质的生物分析研究 互动。建议采用新的工具方法 荧光检测二色性（FDCD）将用作探针 药物/蛋白质相互作用。 FDCD 仪器提供快速 FDCD 数据的采集和增强的解释能力 光学活性多组分荧光混合物。使用这个 用于选择性监测手性猝灭的仪器提供了 研究荧光药物的蛋白质结合的强大工具。 具体来说，整体项目分为三个方面：1）使用 FDCD 测量有助于了解荧光结合 人血清白蛋白（HSA）药物和选择的 HSA 片段； 2） 开发新算法以帮助分析和解释 所获取的数据以及 3) FDCD 测量与 其他荧光参数，如猝灭、去偏振和 寿命，以及其他仪器技术，如红外和核磁共振 为选定的药物结合位点提供更有用的探针 蛋白质。