BIOANALYTICAL SEPARATIONS USING CHIRAL POLYMERS

使用手性聚合物进行生物分析分离

• 批准号: 2518940
• 负责人: ISIAH M WARNER
• 金额: $ 14.01万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2518940
• 关键词: capillary electrophoresis; chemical synthesis; high performance liquid chromatography; micelles; peptides; physical separation; polymers; stereoisomer; surfactant

DESCRIPTION: The research proposed here is designed to study new technologies for improved High Performance Liquid Chromatographic (HPLC) and Capillary Electrophoresis (CE) separation of chiral molecules. This research will focus on three separate objectives involving various aspects of separations by HPLC and CE. The first objective involves the synthesis and evaluation of chiral micelle polymers as mobile phase additives to achieve chiral separation. The second objective involves an evaluation of the use of linear polypeptides as stationary phases to achieve chiral separation. The third component involves the systematic evaluation of combining the two schemes proposed above to achieve improved chiral separations. The proposed research is important to many health related fields, particularly the pharmaceutical and agricultural industries. Recent studies have demonstrated that many drugs exist as racemic mixtures, where one enantiomer may be medicinally beneficial and the other toxic. Improved methodologies for separation of racemic mixtures is the key to understanding such effects and is also the focus of this proposal.

描述：这里提出的研究旨在研究新的 用于改进高效液相色谱（HPLC）的技术 以及手性分子的毛细管电泳（CE）分离。这 研究将集中在三个不同的目标，涉及各种 通过HPLC和CE进行分离的方面。第一个目标涉及 手性胶束聚合物移动的相合成与评价 添加剂以实现手性分离。第二个目标涉及 使用线性多肽作为固定相的评估 实现手性分离。第三个部分涉及系统的 评估结合上述两种方案，以实现 改进的手性分离。这项研究对许多人来说都很重要。 健康相关领域，特别是制药和农业 行业最近的研究表明，许多药物作为 外消旋混合物，其中一种对映异构体可能是药用有益的， 另一种有毒。改进的外消旋体分离方法 混合物是理解这种效应的关键，也是研究的重点 这一提议。