BIOANALYTICAL SEPARATIONS USING CHIRAL POLYMERS

使用手性聚合物进行生物分析分离

• 批准号: 2518940
• 负责人: ISIAH M WARNER
• 金额: $ 14.01万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2518940
• 关键词: capillary electrophoresis; chemical synthesis; high performance liquid chromatography; micelles; peptides; physical separation; polymers; stereoisomer; surfactant

DESCRIPTION: The research proposed here is designed to study new technologies for improved High Performance Liquid Chromatographic (HPLC) and Capillary Electrophoresis (CE) separation of chiral molecules. This research will focus on three separate objectives involving various aspects of separations by HPLC and CE. The first objective involves the synthesis and evaluation of chiral micelle polymers as mobile phase additives to achieve chiral separation. The second objective involves an evaluation of the use of linear polypeptides as stationary phases to achieve chiral separation. The third component involves the systematic evaluation of combining the two schemes proposed above to achieve improved chiral separations. The proposed research is important to many health related fields, particularly the pharmaceutical and agricultural industries. Recent studies have demonstrated that many drugs exist as racemic mixtures, where one enantiomer may be medicinally beneficial and the other toxic. Improved methodologies for separation of racemic mixtures is the key to understanding such effects and is also the focus of this proposal.

描述：此处提出的研究旨在研究新的 改进高性能液相色谱技术（HPLC）的技术 和毛细血管电泳（CE）手性分子的分离。这 研究将集中于三个涉及各种目标的单独目标 HPLC和CE分离的各个方面。第一个目标涉及 手性胶束聚合物作为流动相的合成和评估 添加剂以实现手性分离。第二个目标涉及 评估线性多肽作为固定相的使用 实现手性分离。第三个组件涉及系统 评估上面提出的两个方案以实现的评估 改善了手性分离。拟议的研究对许多人很重要 与健康相关的领域，特别是药物和农业 行业。最近的研究表明，许多药物存在 消极的混合物，一个对映异构体可能是有益的，并且 另一个有毒。改进的方法分离的方法论 混合物是理解这种影响的关键，也是重点 该提议。