BIOANALYSIS--DRUG/PROTEIN INTERACTIONS

生物分析——药物/蛋白质相互作用

• 批准号: 3297088
• 负责人: ISIAH M WARNER
• 金额: $ 4.66万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297088
• 关键词: binding proteins; chemical binding; drug interactions; drug receptors; fluorescence polarization; mathematical model; nuclear magnetic resonance spectroscopy; serum albumin

The research proposed here involves bioanalytical studies of drug/protein interactions. It is proposed that a new instrumental approach to fluorescence detected dichroism (FDCD) will be used as a probe for drug/protein interactions. The FDCD instrumentation provides rapid acquisition of FDCD data and enhanced capabilities for Interpretation of optically active multicomponent fluorescence mixtures. The use of this instrumentation for selectively monitoring chiral quenching provides a powerful tool for studying protein binding of fluorescence drugs. Specifically, the overall project is divided into three areas: 1) the use of FDCD measurements to aid in understanding the binding of fluorescent drugs to human serum albumin (HSA) and select fragments of HSA; 2) the development of novel algorithms to aid in the analysis and interpretation of the acquired data and 3) the combination of FDCD measurement with other parameters of fluorescence such as quenching, depolarization and lifetimes, as well as other instrumental techniques such as IR and NMR to provide a more useful probe of the drug binding sites in selected proteins.

这里提出的研究涉及药物/蛋白质的生物分析研究 交互.提出了一种新的工具性方法， 荧光检测二向色性（FDCD）将被用作探针， 药物/蛋白质相互作用。FDCD仪器提供快速 获取FDCD数据，并增强对 光学活性多组分荧光混合物。使用这种 用于选择性监测手性猝灭的仪器提供了一种 是研究荧光药物与蛋白质结合的有力工具。 具体来说，整个项目分为三个方面：1）使用 的FDCD测量，以帮助了解荧光结合 药物对人血清白蛋白（HSA）和HSA的选择片段; 2） 开发新的算法，以帮助分析和解释 和3）FDCD测量与 荧光的其它参数如猝灭、去偏振和 寿命，以及其他仪器技术，如IR和NMR， 提供了一种更有用的药物结合位点的探针， proteins.