BIOANALYSIS--DRUG/PROTEIN INTERACTIONS

生物分析——药物/蛋白质相互作用

• 批准号: 3297088
• 负责人: ISIAH M WARNER
• 金额: $ 4.66万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297088
• 关键词: binding proteins; chemical binding; drug interactions; drug receptors; fluorescence polarization; mathematical model; nuclear magnetic resonance spectroscopy; serum albumin

The research proposed here involves bioanalytical studies of drug/protein interactions. It is proposed that a new instrumental approach to fluorescence detected dichroism (FDCD) will be used as a probe for drug/protein interactions. The FDCD instrumentation provides rapid acquisition of FDCD data and enhanced capabilities for Interpretation of optically active multicomponent fluorescence mixtures. The use of this instrumentation for selectively monitoring chiral quenching provides a powerful tool for studying protein binding of fluorescence drugs. Specifically, the overall project is divided into three areas: 1) the use of FDCD measurements to aid in understanding the binding of fluorescent drugs to human serum albumin (HSA) and select fragments of HSA; 2) the development of novel algorithms to aid in the analysis and interpretation of the acquired data and 3) the combination of FDCD measurement with other parameters of fluorescence such as quenching, depolarization and lifetimes, as well as other instrumental techniques such as IR and NMR to provide a more useful probe of the drug binding sites in selected proteins.

这里提出的研究涉及药物/蛋白质的生物分析研究 互动。有人提出一种新的工具方法 检测到的荧光二色性（FDCD）将用作探针 药物/蛋白质相互作用。 FDCD仪器可快速 获取FDCD数据和增强功能以​​解释 光学活跃的多组分荧光混合物。使用此 用于选择性监测手性淬火的仪器提供了 用于研究荧光药物蛋白质结合的强大工具。 具体而言，整个项目分为三个领域：1）使用 FDCD测量值以帮助理解荧光的结合 人类血清白蛋白（HSA）的药物和选择HSA的片段； 2） 开发新型算法以帮助分析和解释 获得的数据和3）FDCD测量与 荧光的其他参数，例如淬火，去极化和 终生以及其他乐器技术（例如IR和NMR） 提供对选定的药物结合位点的更有用的探针 蛋白质。