BIOANALYTICAL SEPARATIONS USING CHIRAL POLYMERS

使用手性聚合物进行生物分析分离

• 批准号: 6287520
• 负责人: ISIAH M WARNER
• 金额: $ 1.83万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287520
• 关键词: analytical ultracentrifugation; capillary electrophoresis; chemical aggregate; chemical chain length; chemical synthesis; crosslink; high performance liquid chromatography; hydropathy; infrared spectrometry; interferometry; light scattering; mass spectrometry; mathematical model; micelles; molecular polarity; neutron diffraction; nuclear magnetic resonance spectroscopy; peptides; physical separation; polyglutamates; polymerization; polymers; stereoisomer; surfactant; thermodynamics

DESCRIPTION: (Adapted from the applicant's abstract) This project focuses on synthesis and development of new chiral reagents for use in chiral chromatography for resolution of racemic mixtures. Three different approaches are suggested for this research. The first involves the use of chiral micelle polymers as mobile phase additives to achieve chiral interactions in the mobile phase. The polymeric micelles are produced by photo-induced covalent linkage of the monomers at concentrations above the critical micelle concentration (CMC). These polymers have many of the features of normal micelles except that they do not have a CMC value. Thus, much of the dynamic equilibria which is associated with conventional micelles is eliminated. It is proposed that this property should enhance chiral discrimination in the mobile phase. In addition, much lower polymeric micelle concentrations can be employed compared to that required for traditional surfactant micelles enhancing chiral recognition. The second part of this proposal involves the use of linear polypeptides as stationary phases for chiral separations. It is proposed that the ability of these chiral molecules to exist in well defined chain conformations of extensive order, even in solution, should enhance chiral recognition in the stationary phase. The third component of this research involves a combination of the two approaches proposed, i.e., use of chiral micelle polymers in the mobile phase in conjunction with chiral polypeptides in the stationary phase. The application of this approach to capillary electrochromatography (CEC) opens new avenues for chiral separation.

描述：（改编自申请人的摘要）该项目的重点 新型手性试剂的合成与开发 用于拆分外消旋混合物的色谱法。 三种不同 为这项研究提出了方法。 第一个涉及使用 手性胶束聚合物作为移动的相添加剂， 移动的阶段的交互。 聚合物胶束通过以下方法产生： 单体的光诱导共价键在浓度高于 临界胶束浓度（CMC）。 这些聚合物具有许多 除了它们不具有CMC值之外，它们具有正常胶束的特征。 因此，在本发明中， 许多与传统平衡相关的动态平衡 胶束被消除。 建议该属性应增强 移动的相中的手性鉴别。 此外，低得多的 可以采用聚合物胶束浓度 用于传统表面活性剂胶束增强手性识别。 的 该建议的第二部分涉及使用线性多肽作为 用于手性分离的固定相。 建议能力 这些手性分子存在于明确定义的链构象， 广泛的顺序，即使在溶液中，应该增强手性识别在 固定相 本研究的第三个组成部分涉及一个 所提出的两种方法的组合，即，手性胶束的应用 在移动的相中的聚合物结合在 固定相 该方法在毛细管中的应用 电色谱（CEC）为手性分离开辟了新的途径。