PRODUCTION OF PEDIGREED SPF RHESUS MACAQUES: HIV ANIMAL MODELS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 描述（由申请人提供）： 该提案的目标是扩大具有遗传特征的印度血统恒河猴的生产，这些恒河猴对7种持久性病毒的扩展列表无特异性病原体（SPF），包括猿猴免疫缺陷病毒（SIV）、猿猴D型逆转录病毒（SRV）、猿猴嗜T淋巴细胞病毒（STLV）、猕猴疱疹病毒1型（B病毒）、猿猴泡沫病毒（SFV）、恒河猴巨细胞病毒（RhCMV）和恒河猴鼻状病毒（RRV）。这一拟议的扩展将适合于生产SPF动物用于艾滋病相关研究，并建立一个长期的SPF繁殖群体的印度原产恒河猴与已知的谱系和明确的MHC基因型。为了实现这些目标，我们将通过新生儿的托儿所饲养，从传统的非SPF印度恒河猴繁殖群中获得SPF后代。此外，我们将通过自然繁殖和社会群体中的母亲养育来扩大现有的印度裔群体，该群体是5种持久性病毒（SIV，SRV，STLV，SFV，B病毒）的SPF。扩展程序的MHC分型，包括额外的微卫星位点，I类等位基因，和II类基因将被用来表征后代和他们的父母。这种扩展的MHC分型将允许识别和定义免疫应答基因型，并允许选择育种以产生具有特定遗传特征的动物。我们还建议收集与年龄相关的变化，在队列的主要淋巴细胞亚群的托儿所饲养和母亲饲养的SPF猕猴的纵向数据。从正常、健康的SPF恒河猴中开发出的特异性参考范围对于更充分地理解和利用猕猴模型系统，以及提供比较数据以更准确地解释在AIDS和其他感染性和免疫性疾病中观察到的淋巴细胞亚群表型改变是必不可少的。作为这笔赠款的一部分，恒河猴将为艾滋病相关研究提供宝贵的资源。这些动物将用于与疫苗开发和抗病毒治疗相关的研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The goal of this proposal is to expand the production of genetically characterized rhesus macaques of Indian-ancestry that are specific pathogen-free (SPF) for an expanded list of 7 persistent viruses, including simian immunodeficiency virus (SIV), simian type D retrovirus (SRV), simian T-lymphotropic virus (STLV), Cercopithecine herpesvirus 1 (B virus), simian foamy virus (SFV), rhesus cytomegalovirus (RhCMV) and rhesus rhadinovirus (RRV). This proposed expansion will be geared to the production of SPF animals for use in AIDS-related research, and to establishing a long-term SPF breeding colony of Indian-origin rhesus macaques with known pedigrees and well-defined MHC genotypes. To meet these objectives, we will derive SPF offspring from conventional, non-SPF Indian rhesus breeding stock through nursery-rearing of neonates. In addition, we will expand the existing Indian-origin colony that is SPF for 5 persistent viruses (SIV, SRV, STLV, SFV, B virus) through natural breeding and mother-rearing in social groups. An expanded program of MHC typing that includes additional microsatellite loci, class I alleles, and class II genes will be used to characterize offspring and their parents. This expanded MHC typing will allow the identification and definition of immune response genotypes, and will allow for selected breeding to produce animals with specific genetic profiles. We also propose to collect longitudinal data on age-related changes in the major lymphocyte subsets in cohorts of nursery-reared and mother-reared SPF rhesus macaques. Age-specific reference ranges, developed from normal, healthy SPF-rhesus macaques, are essential to more fully understand and utilize the macaque model system, and to provide comparative data allowing for more accurate interpretation of lymphocyte subset phenotype alterations observed in AIDS and other infectious and immunologic diseases. The rhesus monkeys produced as part of this grant will provide valuable resources for AIDS-related research. These animals will be used for research related to vaccine development and anti-viral therapy.