Using single-cell RNA-seq to interrogate host immunity to pathogens

使用单细胞 RNA-seq 探究宿主对病原体的免疫力

• 批准号: 9367846
• 负责人: John T Chang
• 金额: $ 3.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-02 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9367846
• 关键词: Acute; Bacteria; Bacterial Infections; Biomedical Research; CD8-Positive T-Lymphocytes; Cause of Death; Cells; Communicable Diseases; Development; Exhibits; Gene Expression Profiling; Genetic; Goals; Heterogeneity; Immune system; Immunity; Immunologic Memory; Individual; Infection; Knock-out; Listeria monocytogenes; Lymphocyte; Memory; Messenger RNA; Molecular; Population; RNA Computations; Regulator Genes; Research; Systemic infection; T memory cell; T-Lymphocyte; Transcriptional Regulation; Vaccine Design; Vaccines; Virus; adaptive immune response; adaptive immunity; base; design; improved; in vivo; insight; knock-down; microbial; mortality; neutralizing antibody; novel; novel vaccines; oral infection; pathogen; response; small hairpin RNA; terminally differentiated effector memory (TEM) T cells; transcriptome; transcriptome sequencing; transcriptomics; vaccination strategy

Project Summary Mortality from infectious diseases remains a leading cause of death worldwide, making the development of new vaccines an important priority of biomedical research. Immunologic memory is a cardinal feature of adaptive immunity and an important goal of vaccination strategies. Traditional vaccination strategies are very effective at generating neutralizing antibodies against bacteria and viruses. However, a vaccine capable of generating robust T lymphocyte memory is still beyond our research, due, in part, to an incomplete understanding of the molecular basis of lymphocyte fate specification. In this proposal, we will develop single-cell approaches to study specification of lymphocyte fates in response to microbial infection.

项目摘要 传染病死亡仍然是世界范围内的主要死亡原因， 使新疫苗的开发成为生物医学研究的重要优先事项。 免疫记忆是获得性免疫的一个基本特征，也是 疫苗接种策略。传统的疫苗接种策略在产生 针对细菌和病毒的中和抗体。然而，一种能够 产生强大的T淋巴细胞记忆仍然超出了我们的研究范围，部分原因是 对淋巴细胞命运规范的分子基础认识不全面。在这 提议，我们将开发单细胞方法来研究淋巴细胞的特性 命运对微生物感染的反应。