RNA sequencing (RNA-Seq) of the NCI-60

NCI-60 的 RNA 测序 (RNA-Seq)

• 批准号: 9780250
• 负责人: William Reinhold
• 金额: $ 12.48万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9780250
• 关键词: Area; CCR; Cancerous; Cell Line; Collaborations; DNA; DNA copy number; DNA sequencing; Data; Developmental Therapeutics Program; Funding; Genes; Genomics; Location; Manuscripts; MicroRNAs; Molecular; Online Systems; Pattern; Pharmaceutical Preparations; Pharmacology; Phenotype; Population; Preparation; Publishing; Quality Control; RNA Splicing; Site; Transcript; Variant; Work; biological systems; deep sequencing; genetic variant; tool; tool development; transcriptome sequencing

This project will be particularly informative in the area of transcript expression, including the levels and multiple forms of the transcripts present for each gene. The arrays are being done in collaboration with Paul Metzer (Genomic Branch, CCR). Funding has been provided by James Doroshow (Developmental Therapeutics Program, NCI). The analysis for this project is in progress. When complete, it will allow comparisons to any of our phenotypic, molecular, or pharmacological patterns. Data will be provided to the user in toto, in a queryable format (by gene or location), or as a gene centric "Cell line signature" providing a synopsis of the transcript expression status of a gene. All of these will be accessible on our CellMiner web-based application at http://discover.nci.nih.gov/cellminer/analysis.do. This project is on going and not yet published or available as a public tool. Quality control has determined the work to be technically of good quality, and scientific analysis, tool development, and manuscript preparation are in progress.

该项目将在转录本表达领域提供特别的信息，包括每个基因的转录本的水平和多种形式。这些阵列是与Paul Metzer（CCR基因组分支）合作完成的。该项目由James Doroshow（Developmental Therapeutics Program，NCI）资助。该项目的分析正在进行中。完成后，它将允许与我们的任何表型，分子或药理学模式进行比较。数据将以可查询的格式（按基因或位置）全部提供给用户，或作为以基因为中心的“细胞系签名”提供基因转录本表达状态的概要。所有这些都可以在我们的基于网络的CellMiner应用程序http://discover.nci.nih.gov/cellminer/analysis.do上访问。该项目正在进行中，尚未作为公共工具发布或提供。质量控制已确定这项工作在技术上质量良好，科学分析、工具开发和手稿准备正在进行中。