Development of novel molecular or phenotypic databases

开发新型分子或表型数据库

• 批准号: 10262772
• 负责人: William Reinhold
• 金额: $ 14.3万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10262772
• 关键词: Cell Line; Cells; Complement; DNA Methylation; DNA Sequence Alteration; DNA copy number; Data; Data Set; Databases; Development; Genomics; Goals; Measures; MicroRNAs; Molecular; Mutation; Pattern; Pharmaceutical Preparations; Pharmacology; Phenotype; Proteins; Site; Spectrophotometry; System; Time; Transcript; data tools; molecular phenotype; novel; phenotypic data; tool; transcriptome sequencing; web app

Databases added for the current year include NCI-60 RNA sequencing and SWATH protein (from mass spectrophotometry). From CCLE we have added transcript expression from microarray and RNA sequencing, microRNA expression, DNA mutation, DNA copy number, and drug activity. From GDSC we have added transcript expression from microarray, DNA methylation, DNA mutation, and drug activity. From CTRP we have added drug activities. From NCI/DTP SCLC we have added transcript expression from microarray, and drug activity. Additional forms of data will be explored and organized logically as time and opportunity permits. These datasets will continue to be incorporated into CellMiner and/or CellMinerCDB (https://discover.nci.nih.gov/cellminercdb/) in such a fashion as to make then accessible and usable to the scientific public. This will include each data type being developed into its own "Cell line signature" to facilitate its comparison to other data types, and its inclusion in our comparison and analysis tools to allow their integration with and comparison to our approximately 2.2 x 10E11 cells x phenotypic patterns x molecular patterns. NCI-60 databases will appear in both CellMiner and CellMinerCDB web-applications. The other datasets will appear in CellMinerCDB.

本年度新增的数据库包括NCI-60 RNA测序和SWATH蛋白（来自质量分光光度法）。从CCLE中，我们添加了来自微阵列和RNA测序的转录物表达，microRNA表达，DNA突变，DNA拷贝数和药物活性。从GDSC中，我们添加了来自微阵列的转录物表达，DNA甲基化，DNA突变和药物活性。我们从CTRP中添加了药物活性。从NCI/DTP SCLC中，我们添加了来自微阵列的转录物表达和药物活性。在时间和机会允许的情况下，将探索和逻辑组织其他形式的数据。这些数据集将继续被纳入CellMiner和/或CellMinerCDB （https://discover.nci.nih.gov/cellminercdb/）中，以便科学公众可以访问和使用。这将包括每种数据类型被开发成自己的“细胞系签名”，以便与其他数据类型进行比较，并将其包含在我们的比较和分析工具中，以便与我们的大约2.2 x 10E11细胞x表型模式x分子模式进行整合和比较。NCI-60数据库将出现在CellMiner和CellMinerCDB web应用程序中。其他数据集将出现在CellMinerCDB中。