Microbial and Phage Cultivation Core

微生物和噬菌体培养核心

• 批准号: 10357966
• 负责人: ROBERT A BRITTON
• 金额: $ 40.04万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10357966
• 关键词: Anaerobic Bacteria; Animal Disease Models; Anti-Bacterial Agents; Antibiotic Resistance; Bacteria; Bacterial Antibiotic Resistance; Bacteriophages; Clostridium difficile; Collection; Communities; Complex; Disease; ESKAPE pathogens; Etiology; Evolution; Faculty; Foundations; Generations; Genes; Genome; Genomics; Goals; Health; Homeostasis; Human; Human Microbiome; Human Resources; Human body; Individual; Infrastructure; Intestines; Investments; Laboratories; Libraries; Lytic; Medicine; Metagenomics; Microbe; Mission; Mucous Membrane; Nature; Nose; Organoids; Pathogenicity; Production; Reagent; Research; Resources; Role; Scientist; Sequence Analysis; Services; Site; Surface; Therapeutic; Vagina; Viral; atmospheric conditions; college; combat; cost; data integration; deep sequencing; gene function; host microbiome; human model; innovation; metagenome; metagenomic sequencing; microbial; microbial community; microbial genome; microbiome; microbiome alteration; microbiome research; novel; pathogen; pathogenic bacteria; tool; translational study

PROJECT SUMMARY The overall goal of the BCM-CARBIRU is to explore non-antibiotic therapeutic approaches to combating antibiotic resistant bacterial pathogens that are deemed urgent and serious threats. To meet this goal, the establishment of the Microbial and Phage Cultivation (MPC) core will provide to the projects the ability to cultivate bacteria and phage in multiple formats and under different atmospheric conditions. The core will also provide sequencing support of newly identified bacteria and phage that emerge from each of the three projects. The MPC organized around three primary objectives. Objective 1. Cultivation of complex microbial communities and individual microbes. Objective 2. Isolation and optimization of bacteriophage for AMR pathogens. Objective 3. Sequencing of individual microbes, phage and complex microbial communities. Centralization of these objectives into the MPC core will reduce costs by allowing all of the projects access to the infrastructure (such as anaerobic and microoxic chambers), bulk purchasing of reagents, and the reduction in labor costs by having dedicated technicians to assist in generation of bacteria and phage. The MPC will facilitate the transfer of expertise in microbial and phage cultivation from the core to trainees from all three projects. Finally, a centralized resource for sequencing of bacteria and phage will allow more coordination between the projects in regard to genomic analysis and data integration. The MPC will be an essential part of the BCM-CARBIRU that, along with the Organoid Cultivation Core, will help achieve the overall goals proposed.

项目摘要 BCM-CARBIRU的总体目标是探索非抗生素治疗方法， 抗击被视为紧迫和严重威胁的抗生素耐药性细菌病原体。到 为了实现这一目标，建立微生物和噬菌体培养（MPC）核心将提供 该项目的能力，培养细菌和噬菌体在多种形式和不同的条件下， 大气条件。该核心还将为新鉴定的细菌提供测序支持 和噬菌体的数量。MPC组织了三个主要的 目标. 目的1.复杂微生物群落和单个微生物的培养。 目标2.抗生素耐药性病原体噬菌体的分离和优化。 目标3.单个微生物、噬菌体和复杂微生物群落的测序。 将这些目标集中到MPC核心中将通过允许所有项目 获得基础设施（如厌氧和微氧室）， 试剂，并通过配备专门的技术人员来帮助生成 细菌和噬菌体。MPC将促进微生物和噬菌体方面的专业知识的转让 从核心到所有三个项目的受训人员的培养。最后，集中资源， 细菌和噬菌体的测序将使项目之间在以下方面进行更多的协调： 基因组分析和数据整合。MPC将是BCM-CARBIRU的重要组成部分， 沿着类器官培养核心，将有助于实现所提出的总体目标。