Microbial and Phage Cultivation Core

项目总结 BCM-CARBIRU的总体目标是探索非抗生素治疗方法 与被认为是紧迫和严重威胁的抗药性细菌病原体作斗争。至 为了实现这一目标，微生物和噬菌体培养(MPC)核心的建立将提供 该项目能够以多种形式和不同的方式培养细菌和噬菌体 大气条件。该核心还将为新发现的细菌提供测序支持 以及从三个项目中每一个出现的噬菌体。货币政策委员会围绕三个初选组织了 目标。 目的1.复合微生物群落和单个微生物的培养。 目的2.AMR病原菌噬菌体的分离和优化。 目的3.单个微生物、噬菌体和复合微生物群落的测序。 将这些目标集中到MPC核心将通过允许所有项目 进入基础设施(如厌氧室和微氧室)，批量购买 试剂，并通过有专门的技术人员协助生成 细菌和噬菌体。MPC将促进微生物和噬菌体方面的专业知识的转移 从核心培养到三个项目的实习生。最后，集中资源用于 细菌和噬菌体的测序将允许项目之间在以下方面进行更多的协调 基因组分析和数据整合。MPC将是BCM-CARBIRU的重要组成部分， 随着有机化合物培育的核心，将有助于实现所提出的总体目标。

PROJECT SUMMARY The overall goal of the BCM-CARBIRU is to explore non-antibiotic therapeutic approaches to combating antibiotic resistant bacterial pathogens that are deemed urgent and serious threats. To meet this goal, the establishment of the Microbial and Phage Cultivation (MPC) core will provide to the projects the ability to cultivate bacteria and phage in multiple formats and under different atmospheric conditions. The core will also provide sequencing support of newly identified bacteria and phage that emerge from each of the three projects. The MPC organized around three primary objectives. Objective 1. Cultivation of complex microbial communities and individual microbes. Objective 2. Isolation and optimization of bacteriophage for AMR pathogens. Objective 3. Sequencing of individual microbes, phage and complex microbial communities. Centralization of these objectives into the MPC core will reduce costs by allowing all of the projects access to the infrastructure (such as anaerobic and microoxic chambers), bulk purchasing of reagents, and the reduction in labor costs by having dedicated technicians to assist in generation of bacteria and phage. The MPC will facilitate the transfer of expertise in microbial and phage cultivation from the core to trainees from all three projects. Finally, a centralized resource for sequencing of bacteria and phage will allow more coordination between the projects in regard to genomic analysis and data integration. The MPC will be an essential part of the BCM-CARBIRU that, along with the Organoid Cultivation Core, will help achieve the overall goals proposed.