Cell and Tissue Analysis Core

细胞和组织分析核心

• 批准号: 10646153
• 负责人: Richard KP Benninger
• 金额: $ 17.72万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10646153
• 关键词: Bioinformatics; Cells; Color; Colorado; Confocal Microscopy; Custom; Cytometry; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Dedications; Device or Instrument Development; Diabetes Mellitus; Educational workshop; Equipment; Event; Experimental Designs; Faculty; Flow Cytometry Shared Resource; Fluorescence Microscopy; Goals; Grant; Human; Image; Image Analysis; Immunology; Investments; Journals; Lipids; Maintenance; Medical; Metabolism; Microscope; Microscopy; Mission; Multiplexed Ion Beam Imaging; Nanoscopy; Preparation; Productivity; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Resources; Running; Sampling; Series; Signal Transduction; Sorting; Specialist; Speed; System; Teacher Professional Development; Technology; Tissues; Training; Universities; Validation; antibody libraries; cost; data handling; human imaging; imaging platform; innovation; instrumentation; intravital imaging; light microscopy; member; microscopic imaging; tool; two photon microscopy; two-photon; ultra high resolution

CELL AND TISSUE ANALYSIS CORE PROJECT SUMMARY/ABSTRACT The overall objective of the Cell and Tissue Analysis Core is to provide diabetes researchers at the University of Colorado with a unified means to access and effectively use state-of-the art microscopy and mass cytometry systems to drive innovative research. This necessarily includes providing access to this equipment; expertise and training to use the equipment effectively for diabetes-related tissues; expertise and validated diabetes- related resources to prepare samples appropriately; guidance to design experiments; resources to analyze data; and assistance with its interpretation. Fluorescence microscopy and multi-channel cytometric approaches are related tools that are central to diabetes research. The availability of higher-end systems that provide important functionalities is often limited by: 1) the high cost of the systems; 2) the costs and expertise needed to maintain these systems; 3) the specialist expertise needed to use these systems to effectively collect data; and 4) validated reagents for diabetes-related analysis. This presents a challenge for diabetes researchers to incorporate state-of-the-art microscopy and cytometry approaches in their research. The University of Colorado Anschutz Medical campus possesses state-of-the-art microscopy systems and mass-cytometry systems that have been acquired through internal investment, external shared instrumentation grants or instrument development grants. There is also tremendous expertise from expert investigators and highly trained staff. DRC support provides an opportunity to leverage these resources for diabetes researchers. The mission of this core is to provide access and enable effective usage of these sophisticated systems so that diabetes researchers can incorporate innovative approaches, to both maximize research productivity and catalyze new research directions. We will achieve these goals through the following aims: AIM 1: Enable access to state-of-the-art high-resolution multi-color confocal microscopy for diabetes researchers; AIM 2: Provide expert assistance to incorporate new advanced microscopy approaches to enhance diabetes research; AIM 3: Provide access to, and expert assistance for, state-of-the-art mass cytometry and multiplexed ion beam imaging for diabetes-related applications; AIM 4: Train diabetes researchers in incorporating new imaging and cytometry technologies in their research.

细胞和组织分析核心 项目摘要/摘要 细胞和组织分析核心的总体目标是为加州大学的糖尿病研究人员提供 科罗拉多州拥有统一的访问和有效使用最先进的显微镜和质量细胞术的手段 推动创新研究的系统。这必然包括提供对该设备的访问；专业知识 和培训，以便有效地将设备用于糖尿病相关组织；专业知识和有效的糖尿病- 适当准备样品的相关资源；设计实验的指导；分析数据的资源； 并协助其解释。荧光显微镜和多通道细胞术的方法是 对糖尿病研究至关重要的相关工具。高端系统的可用性，可提供重要的 功能通常受到以下因素的限制：1)系统的高成本；2)维护所需的成本和专业知识 这些系统；3)使用这些系统有效收集数据所需的专业知识；以及4) 用于糖尿病相关分析的有效试剂。这对糖尿病研究人员提出了一个挑战 在他们的研究中融入了最先进的显微镜和细胞学方法。科罗拉多大学 Anschutz医学院拥有最先进的显微镜系统和质量细胞测量系统， 通过内部投资、外部共享工具赠款或工具获得 发展赠款。还有来自专家调查人员和训练有素的工作人员的丰富专业知识。刚果民主共和国 支持为糖尿病研究人员提供了利用这些资源的机会。这个核心的使命是 是提供访问并有效使用这些复杂的系统，以便糖尿病研究人员能够 采用创新的方法，既最大限度地提高研究生产率，又催化新的研究方向。 我们将通过以下目标实现这些目标：目标1：使人们能够获得最先进的高分辨率 糖尿病研究人员的多色共聚焦显微镜；目标2：提供专家协助，纳入新的 加强糖尿病研究的先进显微镜方法；目标3：提供访问和专家 辅助最先进的质量细胞术和多路离子束成像治疗糖尿病相关疾病 应用；目标4：培训糖尿病研究人员将新的成像和细胞分析技术纳入其 研究。