Cell and Tissue Analysis Core

细胞和组织分析核心

• 批准号: 10646153
• 负责人: Richard KP Benninger
• 金额: $ 17.72万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10646153
• 关键词: Bioinformatics; Cells; Color; Colorado; Confocal Microscopy; Custom; Cytometry; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Dedications; Device or Instrument Development; Diabetes Mellitus; Educational workshop; Equipment; Event; Experimental Designs; Faculty; Flow Cytometry Shared Resource; Fluorescence Microscopy; Goals; Grant; Human; Image; Image Analysis; Immunology; Investments; Journals; Lipids; Maintenance; Medical; Metabolism; Microscope; Microscopy; Mission; Multiplexed Ion Beam Imaging; Nanoscopy; Preparation; Productivity; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Resources; Running; Sampling; Series; Signal Transduction; Sorting; Specialist; Speed; System; Teacher Professional Development; Technology; Tissues; Training; Universities; Validation; antibody libraries; cost; data handling; human imaging; imaging platform; innovation; instrumentation; intravital imaging; light microscopy; member; microscopic imaging; tool; two photon microscopy; two-photon; ultra high resolution

CELL AND TISSUE ANALYSIS CORE PROJECT SUMMARY/ABSTRACT The overall objective of the Cell and Tissue Analysis Core is to provide diabetes researchers at the University of Colorado with a unified means to access and effectively use state-of-the art microscopy and mass cytometry systems to drive innovative research. This necessarily includes providing access to this equipment; expertise and training to use the equipment effectively for diabetes-related tissues; expertise and validated diabetes- related resources to prepare samples appropriately; guidance to design experiments; resources to analyze data; and assistance with its interpretation. Fluorescence microscopy and multi-channel cytometric approaches are related tools that are central to diabetes research. The availability of higher-end systems that provide important functionalities is often limited by: 1) the high cost of the systems; 2) the costs and expertise needed to maintain these systems; 3) the specialist expertise needed to use these systems to effectively collect data; and 4) validated reagents for diabetes-related analysis. This presents a challenge for diabetes researchers to incorporate state-of-the-art microscopy and cytometry approaches in their research. The University of Colorado Anschutz Medical campus possesses state-of-the-art microscopy systems and mass-cytometry systems that have been acquired through internal investment, external shared instrumentation grants or instrument development grants. There is also tremendous expertise from expert investigators and highly trained staff. DRC support provides an opportunity to leverage these resources for diabetes researchers. The mission of this core is to provide access and enable effective usage of these sophisticated systems so that diabetes researchers can incorporate innovative approaches, to both maximize research productivity and catalyze new research directions. We will achieve these goals through the following aims: AIM 1: Enable access to state-of-the-art high-resolution multi-color confocal microscopy for diabetes researchers; AIM 2: Provide expert assistance to incorporate new advanced microscopy approaches to enhance diabetes research; AIM 3: Provide access to, and expert assistance for, state-of-the-art mass cytometry and multiplexed ion beam imaging for diabetes-related applications; AIM 4: Train diabetes researchers in incorporating new imaging and cytometry technologies in their research.

细胞和组织分析核心 项目概要/摘要 细胞和组织分析核心的总体目标是为大学的糖尿病研究人员提供 科罗拉多州拥有统一的手段来访问和有效使用最先进的显微镜和质谱流式细胞术 推动创新研究的系统。这必然包括提供对该设备的访问；专业知识 以及有效使用设备治疗糖尿病相关组织的培训；专业知识和经过验证的糖尿病- 适当准备样品的相关资源；指导设计实验；分析数据的资源； 并协助其解释。荧光显微镜和多通道细胞计数方法 对糖尿病研究至关重要的相关工具。提供重要功能的高端系统的可用性 功能通常受到以下因素的限制：1）系统成本高； 2）维护所需的成本和专业知识 这些系统； 3）使用这些系统有效收集数据所需的专业知识；和 4) 用于糖尿病相关分析的经过验证的试剂。这对糖尿病研究人员提出了挑战 在他们的研究中结合了最先进的显微镜和细胞计数方法。科罗拉多大学 安舒茨医学院拥有最先进的显微镜系统和质谱流式细胞术系统， 已通过内部投资、外部共享仪器赠款或仪器获得 发展补助金。专家调查人员和训练有素的工作人员也拥有丰富的专业知识。刚果民主共和国 支持为糖尿病研究人员提供了利用这些资源的机会。这个核心的使命 是提供访问并有效使用这些复杂的系统，以便糖尿病研究人员能够 结合创新方法，最大限度地提高研究生产力并促进新的研究方向。 我们将通过以下目标实现这些目标： 目标 1：实现最先进的高分辨率 供糖尿病研究人员使用的多色共聚焦显微镜；目标 2：提供专家协助以纳入新的 加强糖尿病研究的先进显微镜方法；目标 3：提供接触专家的机会 为糖尿病相关疾病提供最先进的质谱流式细胞术和多重离子束成像援助 应用程序；目标 4：培训糖尿病研究人员将新的成像和细胞计数技术融入到他们的研究中 研究。