Cell and Tissue Analysis Core

细胞和组织分析核心

• 批准号: 10392980
• 负责人: Richard KP Benninger
• 金额: $ 18.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10392980
• 关键词: Bioinformatics; Cells; Color; Colorado; Confocal Microscopy; Custom; Cytometry; Data; Data Analyses; Data Collection; Data Storage and Retrieval; Device or Instrument Development; Diabetes Mellitus; Educational workshop; Equipment; Event; Experimental Designs; Faculty; Flow Cytometry Shared Resource; Fluorescence Microscopy; Goals; Grant; Human; Image; Image Analysis; Image Cytometry; Immunology; Investments; Journals; Lipids; Maintenance; Medical; Metabolism; Microscope; Microscopy; Mission; Multiplexed Ion Beam Imaging; Nanoscopy; Preparation; Productivity; Reagent; Research; Research Personnel; Resolution; Resource Sharing; Resources; Running; Sampling; Series; Signal Transduction; Sorting - Cell Movement; Specialist; Speed; System; Teacher Professional Development; Technology; Tissues; Training; Universities; Validation; antibody libraries; cost; data handling; design; experimental study; human imaging; imaging platform; innovation; instrumentation; intravital imaging; light microscopy; member; microscopic imaging; tool; two photon microscopy; two-photon

CELL AND TISSUE ANALYSIS CORE PROJECT SUMMARY/ABSTRACT The overall objective of the Cell and Tissue Analysis Core is to provide diabetes researchers at the University of Colorado with a unified means to access and effectively use state-of-the art microscopy and mass cytometry systems to drive innovative research. This necessarily includes providing access to this equipment; expertise and training to use the equipment effectively for diabetes-related tissues; expertise and validated diabetes- related resources to prepare samples appropriately; guidance to design experiments; resources to analyze data; and assistance with its interpretation. Fluorescence microscopy and multi-channel cytometric approaches are related tools that are central to diabetes research. The availability of higher-end systems that provide important functionalities is often limited by: 1) the high cost of the systems; 2) the costs and expertise needed to maintain these systems; 3) the specialist expertise needed to use these systems to effectively collect data; and 4) validated reagents for diabetes-related analysis. This presents a challenge for diabetes researchers to incorporate state-of-the-art microscopy and cytometry approaches in their research. The University of Colorado Anschutz Medical campus possesses state-of-the-art microscopy systems and mass-cytometry systems that have been acquired through internal investment, external shared instrumentation grants or instrument development grants. There is also tremendous expertise from expert investigators and highly trained staff. DRC support provides an opportunity to leverage these resources for diabetes researchers. The mission of this core is to provide access and enable effective usage of these sophisticated systems so that diabetes researchers can incorporate innovative approaches, to both maximize research productivity and catalyze new research directions. We will achieve these goals through the following aims: AIM 1: Enable access to state-of-the-art high-resolution multi-color confocal microscopy for diabetes researchers; AIM 2: Provide expert assistance to incorporate new advanced microscopy approaches to enhance diabetes research; AIM 3: Provide access to, and expert assistance for, state-of-the-art mass cytometry and multiplexed ion beam imaging for diabetes-related applications; AIM 4: Train diabetes researchers in incorporating new imaging and cytometry technologies in their research.

细胞和组织分析核心 项目摘要/摘要 细胞和组织分析核心的总体目标是为大学提供糖尿病研究人员 科罗拉多州具有统一手段，可访问和有效使用最先进的显微镜和质量细胞术 推动创新研究的系统。这必然包括提供对该设备的访问；专业知识 并训练有效地将设备用于与糖尿病相关的组织；专业知识和经过验证的糖尿病 - 相关资源以适当准备样品；设计实验的指导；分析数据的资源； 并协助其解释。荧光显微镜和多通道细胞仪方法是 糖尿病研究中心的相关工具。提供重要的高端系统的可用性 功能通常受到以下限制：1）系统的高成本； 2）维护所需的成本和专业知识 这些系统； 3）使用这些系统有效收集数据所需的专业知识；和4） 经过验证的试剂用于与糖尿病有关的分析。这给糖尿病研究人员带来了挑战 将最新的显微镜和细胞术方法纳入其研究中。科罗拉多大学 Anschutz Medical Campus拥有最先进的显微镜系统和质量仪系统 已经通过内部投资，外部共享仪器赠款或工具获取 发展补助金。专家调查员和训练有素的员工也有巨大的专业知识。刚果 支持为糖尿病研究人员提供了一个机会，以利用这些资源。这个核心的任务 是为这些复杂的系统提供访问和有效使用，以便研究人员可以 结合创新方法，以最大化研究生产力并催化新的研究方向。 我们将通过以下目标实现这些目标：目标1：启用最先进的高分辨率 糖尿病研究人员的多色共聚焦显微镜；目标2：提供专家帮助以纳入新 高级显微镜方法增强糖尿病研究；目标3：提供访问和专家 帮助糖尿病相关的最先进的质量细胞仪和多路复用离子束成像 申请；目标4：火车糖尿病研究人员在将新成像和细胞术技术纳入其中 研究。