Cell and Tissue Analysis Core

细胞和组织分析核心 项目概要/摘要 细胞和组织分析核心的总体目标是为糖尿病研究人员在大学提供 科罗拉多，以统一的方式访问和有效使用最先进的显微镜和质谱仪 推动创新研究的系统。这必然包括提供使用这种设备的机会;专门知识 和培训，以有效地使用设备的糖尿病相关组织;专业知识和验证的糖尿病- 适当准备样品的相关资源;设计实验的指导;分析数据的资源; 并协助其解释。荧光显微镜和多通道细胞计数方法是 相关工具对糖尿病研究至关重要。高端系统的可用性， 功能通常受限于：1）系统的高成本; 2）维护所需的成本和专业知识 这些系统; 3）使用这些系统有效收集数据所需的专业知识;以及4） 用于糖尿病相关分析的经过验证的试剂。这对糖尿病研究人员提出了挑战， 将最先进的显微镜和细胞计数方法纳入其研究中。科罗拉多大学 安舒茨医学院拥有最先进的显微镜系统和质谱仪系统， 已通过内部投资、外部共享工具赠款或工具 发展赠款。专家调查员和训练有素的工作人员也提供了大量的专门知识。DRC 支持为糖尿病研究人员提供了利用这些资源的机会。这个核心的使命 是提供这些复杂系统的访问和有效使用，以便糖尿病研究人员可以 采用创新方法，以最大限度地提高研究生产力并促进新的研究方向。 我们将通过以下目标来实现这些目标：目标1：能够获得最先进的高分辨率 为糖尿病研究人员提供多色共聚焦显微镜; AIM 2：提供专家协助， 先进的显微镜方法，以加强糖尿病研究;目的3：提供访问，和专家 协助最先进的质谱仪和多路离子束成像， 目标4：培训糖尿病研究人员将新的成像和细胞术技术应用于他们的研究。 research.

CELL AND TISSUE ANALYSIS CORE PROJECT SUMMARY/ABSTRACT The overall objective of the Cell and Tissue Analysis Core is to provide diabetes researchers at the University of Colorado with a unified means to access and effectively use state-of-the art microscopy and mass cytometry systems to drive innovative research. This necessarily includes providing access to this equipment; expertise and training to use the equipment effectively for diabetes-related tissues; expertise and validated diabetes- related resources to prepare samples appropriately; guidance to design experiments; resources to analyze data; and assistance with its interpretation. Fluorescence microscopy and multi-channel cytometric approaches are related tools that are central to diabetes research. The availability of higher-end systems that provide important functionalities is often limited by: 1) the high cost of the systems; 2) the costs and expertise needed to maintain these systems; 3) the specialist expertise needed to use these systems to effectively collect data; and 4) validated reagents for diabetes-related analysis. This presents a challenge for diabetes researchers to incorporate state-of-the-art microscopy and cytometry approaches in their research. The University of Colorado Anschutz Medical campus possesses state-of-the-art microscopy systems and mass-cytometry systems that have been acquired through internal investment, external shared instrumentation grants or instrument development grants. There is also tremendous expertise from expert investigators and highly trained staff. DRC support provides an opportunity to leverage these resources for diabetes researchers. The mission of this core is to provide access and enable effective usage of these sophisticated systems so that diabetes researchers can incorporate innovative approaches, to both maximize research productivity and catalyze new research directions. We will achieve these goals through the following aims: AIM 1: Enable access to state-of-the-art high-resolution multi-color confocal microscopy for diabetes researchers; AIM 2: Provide expert assistance to incorporate new advanced microscopy approaches to enhance diabetes research; AIM 3: Provide access to, and expert assistance for, state-of-the-art mass cytometry and multiplexed ion beam imaging for diabetes-related applications; AIM 4: Train diabetes researchers in incorporating new imaging and cytometry technologies in their research.