Research Services Core (RSC)

研究服务核心 (RSC)

• 批准号: 10682313
• 负责人: Samuel Michael
• 金额: $ 431.23万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10682313
• 关键词: Acoustics; Aptitude; Archives; Automation; Biological; Biological Assay; Biology; COVID-19; Cells; Chemical Structure; Chemicals; Collaborations; Collection; Communities; Complement; Complex; Computer software; Core Facility; Custom; Data Analyses; Databases; Development; Devices; Dose; Emerging Technologies; Engineering; Ensure; Equipment; Funding; Generations; Hybrids; Incubators; Institutes; Internet; Intramural Research Program; Laboratories; Libraries; Liquid substance; Location; Maintenance; Methods; Mission; Monitor; National Center for Advancing Translational Sciences; National Institute of Allergy and Infectious Disease; Nature; Prevention program; Process; Productivity; Protocols documentation; RNA Interference; RNA interference screen; Rapid screening; Reader; Reagent; Recording of previous events; Research; Research Personnel; Research Project Grants; Research Support; Resources; Retrieval; Robotics; Running; Sampling; Schedule; Scientist; Services; Ships; Support System; System; Technology; Time; Toxicology; Training; Translations; Transportation; United States National Institutes of Health; Vial device; Viral; Work; arm; assay development; cohesion; data quality; data sharing; design; detection assay; detection method; flexibility; follow-up; high throughput screening; imager; instrument; instrumentation; member; new technology; next generation; operation; programs; response; screening; small molecule; stem cells; tissue culture; tool; vigilance

Research Services Core (RSC)staff provide support to the NCATS activities in high-throughput screening, assay development, and optimization as well as follow-up and dose-response library-plating services for NCATS screening activities. The Compound Management team works in conjunction with the Automation team to provide follow-up and dose-response library-plating services for NCATS screening activities. Systems supported by the Automation team include primary screening system, Tox21 screening system, RNAi screening system, the RNAi hit picking system for assay follow ups, and multiple LabCyte Access acoustic plating systems. In the past year, we have also installed and begun to operate a LabCyte Access system to perform high throughput screens in Building 33 in a collaboration with NIAID. Together, all these systems combined provide a wide variety of automation and compound plating tasks used to enable all facets of the NCATS intramural scientific program. The primary screening system utilizes three robotic arms for plate transportation, a storage capacity of more than 3 million compound wells (approximately 2,000 1,536-well compound plates), and more than 1.5 million assay wells (approximately 1,100 1,536-well assay plates) to achieve a world-class screening productivity while maintaining the high level of data quality required by researchers. It also can run multiple assays in parallel. Center experts have developed custom software to monitor and control the system, in addition to archiving the complete process history for every screen in real time. The Tox21 screening system uses a single robotic arm for plate transportation and a storage capacity of nearly 1 million compound or assay wells (about 600 1,536-well compound or assay plates) to screen smaller scale compound libraries not present on the primary screening system and the Tox21 compound library. This platform is designed to complement the primary system, and it is easily customizable, allowing for the rapid integration of additional screening technologies. The RSC team additionally provides screening system services to intramural programs within NCATS. The Tox21 screening system contains a single robotic arm for plate transportation and a storage capacity of about 1.5 million compound wells (about 1,000 1,536-well compound plates) and more than 1.5 million assay wells (approximately 1,100 1,536-well assay plates). With a pin tool and two acoustic dispensers for compound addition, four low-volume dispensers for reagent addition, and three plate readers enabling a variety of assay detection methods, this system can rapidly screen, in triplicate, the Toxicology in the 21st Century (Tox21) library of 10,000 compounds. NCATS experts monitor and control this system with the same custom software used for the primary screening system. The RNAi screening system has two robotic arms for plate transportation with incubators for assay plate storage and plate stackers to facilitate continuous system operation. A multichannel pipettor, low volume dispensers, an aspirator for plate washing, and two different plate readers support a wide variety of detection methods for this RNAi-focused system. RSC staff also continue to support the Stem Cell Translation Laboratory (SCTL). Various team members have provided a large amount of support with a vast array of equipment including automated tissue culture systems, flow cytometers, cell sorters, and high content imagers. Special mention should be given to the automated tissue culture platform which has established itself as a core capability for all NCATS researchers now with the demand becoming so great that another system is most likely on order. These resources require a tremendous amount of expertise to operate and maintain. In the past year, RSC has begun to act as a core facility for these technologies, enabling different biology groups to increase their capabilities. Related to the high content imagers, more high content assays were run in the past year due to the increased capabilities as compared to the previous four years combined. In the past year, we added a 3rd automated tissue culture device to further increase our capacity in this space, and have configured a 4th, more automation friendly instrument from CellTrio, that is on schedule to be delivered and installed later next year. This year also finally saw our new Compound Management space setup after construction and renovations were complete. The Compound Management group moved from their previous B2 location to C1, which was a huge effort given the sheer volume of equipment, all the while ensuring that there was continued operation. In addition to the move, several critical pieces of equipment that had been ordered years prior were finally delivered and installed, including 2 Brooks Sample Store II -20C storage systems, which can be integrated with sample handling and LabCyte Echo plating systems to provide fully automated assay plate generation. This gives NCATS incredible capacity for both compound storage and flexibility to ship assay ready plates with custom plated libraries to collaborators around the world if required. The NCATS intramural program is heavily involved in COVID-19 relief efforts across the NIH. RSC is directly involved in several of these key initiatives, providing project management, scientific and technical support across several programs. Below are a list of some of the COVID-19 projects RSC has worked on: Antiviral Prevention Program (APP) laboratory standup: NCATS received a substantial amount of funding as part of the APP, with many or responsibilities related to high throughput screening, compound management (including anti-viral library design and compound acquisition), assay development and design, and data sharing. RSC was involved in all these activities, helping to fit out an entire lab worth of equipment including instrument selection, ordering, setup, and operation. This was a huge amount of work given the sheer volume of equipment, and in working closely with NCATS building services stood up an entire lab to initiate work for APP in a matter of months.

研究服务核心 (RSC) 工作人员为 NCATS 活动提供高通量筛选、测定开发和优化的支持，以及 NCATS 筛选活动的后续和剂量反应文库电镀服务。化合物管理团队与自动化团队合作，为 NCATS 筛选活动提供后续和剂量反应库电镀服务。自动化团队支持的系统包括初级筛查系统、Tox21 筛查系统、RNAi 筛查系统、用于后续分析的 RNAi 命中挑选系统以及多个 LabCyte Access 声学电镀系统。去年，我们还与 NIAID 合作安装并开始运行 LabCyte Access 系统，以在 33 号楼进行高通量筛选。 所有这些系统结合起来提供了各种各样的自动化和复合电镀任务，用于实现 NCATS 校内科学计划的所有方面。 初级筛选系统利用三个机械臂进行板运输，存储容量超过300万个复合孔（约2,000个1,536孔复合板）和超过150万个分析孔（约1,100个1,536孔分析板），以实现世界一流的筛选生产力，同时保持研究人员所需的高水平数据质量。它还可以并行运行多个测定。除了实时存档每个屏幕的完整过程历史记录外，中心专家还开发了定制软件来监视和控制系统。 Tox21 筛选系统使用单个机械臂进行板运输，并具有近 100 万个化合物或分析孔（约 600 个 1,536 孔化合物或分析板）的存储容量，以筛选初级筛选系统和 Tox21 化合物库中不存在的较小规模的化合物库。该平台旨在补充主系统，并且可以轻松定制，从而可以快速集成其他筛选技术。 RSC 团队还为 NCATS 内的校内项目提供筛查系统服务。 Tox21 筛选系统包含一个用于板运输的机械臂，存储容量为约 150 万个化合物孔（约 1,000 个 1,536 孔化合物板）和超过 150 万个分析孔（约 1,100 个 1,536 孔分析板）。该系统配有一个针工具和两个用于添加化合物的声学分配器、四个用于添加试剂的小容量分配器以及三个可实现多种化验检测方法的读板器，可以快速筛选 21 世纪毒理学 (Tox21) 库中的 10,000 种化合物，一式三份。 NCATS 专家使用与初级筛查系统相同的定制软件来监控和控制该系统。 RNAi 筛选系统有两个用于板运输的机械臂，以及用于存储分析板的培养箱和板堆垛机，以促进系统的连续运行。多通道移液器、低容量分配器、用于洗板的吸气器和两个不同的读板器支持这种以 RNAi 为重点的系统的多种检测方法。 RSC 工作人员还继续支持干细胞翻译实验室 (SCTL)。各个团队成员通过大量设备提供了大量支持，包括自动化组织培养系统、流式细胞仪、细胞分选仪和高内涵成像仪。 特别值得一提的是自动化组织培养平台，该平台已成为所有 NCATS 研究人员的核心能力，现在需求变得如此之大，以至于很可能会订购另一个系统。 这些资源需要大量的专业知识来操作和维护。去年，RSC 已开始充当这些技术的核心设施，使不同的生物学小组能够提高其能力。 与高内涵成像仪相关，由于与前四年的总和相比，能力有所增强，因此去年进行了更多的高内涵测定。 去年，我们添加了第三台自动化组织培养设备，以进一步提高我们在该领域的产能，并配置了 CellTrio 的第四台自动化程度更高的仪器，该仪器按计划将于明年晚些时候交付和安装。 今年，在施工和装修完成后，我们终于看到了新的复合管理空间设置。 院落管理小组从之前的 B2 位置搬到了 C1，考虑到设备数量巨大，这是一项巨大的工作，同时还要确保持续运行。 除了搬迁之外，几年前订购的几台关键设备终于交付并安装，包括 2 个 Brooks Sample Store II -20C 存储系统，该系统可以与样品处理和 LabCyte Echo 电镀系统集成，以提供全自动检测板生成。 这为 NCATS 提供了令人难以置信的化合物存储能力和灵活性，可以根据需要向世界各地的合作者运送带有定制电镀库的检测准备板。 NCATS 校内计划积极参与 NIH 范围内的 COVID-19 救援工作。 RSC 直接参与其中多项关键举措，为多个项目提供项目管理、科学和技术支持。 以下是 RSC 参与的一些 COVID-19 项目的列表： 抗病毒预防计划 (APP) 实验室站立：作为 APP 的一部分，NCATS 获得了大量资金，其许多职责与高通量筛选、化合物管理（包括抗病毒库设计和化合物采集）、测定开发和设计以及数据共享相关。 RSC 参与了所有这些活动，帮助装备整个实验室的设备，包括仪器选择、订购、设置和操​​作。 考虑到设备数量庞大，这是一项巨大的工作量，并且在与 NCATS 建筑服务部门的密切合作下，整个实验室在几个月内就启动了 APP 的工作。