Research Services Core (RSC)

1.自动化 A.两个被接受的自动烧瓶清洗设备设计的第一阶段提案。2个接受了针对罕见疾病的增强现实的第二阶段SBIR建议。 B.仪表和自动化调度现场统计自动化调度总共有142个预订，185个协议正在运行，注册用户总数超过80个。仪表站共保留了112个独立设备(仅限于Outlook溢出的设备，但还在扩大)，还有133个维护请求，要求报告200多个注册用户的设备问题(增加)。 C.10个复杂的机器人平台(从初级筛选机器人到全自动BSL-3系统)和至少250台常规使用的设备，包括针具转移设备、电磁阀、小容量液体分配器、散装分配器和垫圈、声学转移设备、机械臂、孵化器、离心机、封板机和削皮机，由RSC进行维护，节省了数十万美元。 D.与DPI ETB成员和Ariment Robotics合作，帮助设计、构建和实施自动化友好型仪器，以测量和收集实时细胞热位移分析(RT-CETSA)的数据。设计和测试阶段已完成，工厂验收测试计划于2023年9月进行。 2.复合式管理 A.根据NCATS复合管理前沿需求设计、定制和指导的自动管重新格式化和声学点胶系统。我们的复合库集成了一个直接从储存管中取出的声学分配器和一个复杂的液体处理平台，以及用于印版准备/保存和拆封/封口功能的必要外围设备，通过创建检测就绪的印版实现了全自动取管，同时消除了额外的中间进样步骤，从而根据工艺将塑料消耗量减少了1/3至1/2。安装、现场验收测试和培训完成。正在进行验证。 史上最忙碌的一年。为DPI生物小组和合作者填写了2236个Fot订单。79%的化合物要求在国内交付，21%的化合物需要在全国各地交付。 C.为771个订单生产了4612个板材(声学的占40%，非声学的占60%)。其中药物联合钢板占65%，随访钢板占27%，qHTS型钢板占8%。51%的声学斑点板是在我们的多个自动化声学平台上生产的。 3.自动化蜂窝技术 A.CellTrio系统：RoboCell由NCATS细胞技术设计、定制和指导，尖端技术需要在现有的Sartorius紧凑型精选单元的基础上增加能力和规模。包括烧瓶处理、开封/盖盖、检测器、融合检查/成像、复杂液体处理和分配以及储存。工厂验收测试正在进行中，以确保必要的功能。 B.针对神经突触受体、星形胶质细胞、滋养层细胞、下丘脑神经元的五种机器人高通量生物制造和功能分化过程，以及患者IPSCs的系统适应和清理。支持内部(干细胞、3DTBPL等)和众多外部合作者。涉及到科学家对过程和评估的直接参与和反馈。 C.从12个IPSC系产生的伤害性感受器细胞库分为792个冷冻瓶，每瓶包含5M个细胞=3.96B个伤害性感受器细胞。合作者包括波士顿儿童医院、哈佛医学院、神经生物学系、达纳·法伯癌症研究所、癌症生物系和牛津大学纳菲尔德临床神经科学系。从14个IPSC系培养出的星形胶质细胞库共392个，每瓶含0.4M个10M个星形胶质细胞，相当于2.5B个。从3个IPSC系分化出的下丘脑神经元组成91个冷冻箱，每瓶含5M个细胞/瓶=455M个。

1. Automation a. Two accepted phase 1 proposals for an Automated Flask Cleaning device design. 2 accepted phase 2 SBIR proposals for Augmented Reality for rare diseases. b. Instrumentation and Automation scheduling site stats Automation Scheduling had a total of 142 reservations, 185 protocols were run, 80+ total users registered. Instrumentation site had a total of 112 reservations for stand-alone devices (limited to overflow equipment from Outlook but expanding), there were also 133 maintenance requests to report issues on equipment (increasing) with 200+ registered users. c. 10 complex robotic platforms (ranging from the Primary screening robot to the fully automated BSL-3 system) and at least 250 pieces of regularly used equipment comprised of pintool transfer devices, solenoid valve small volume liquid dispensers, bulk dispensers and washers, acoustic transfer devices, robotic arms, incubators, centrifuges, plate sealers and peelers are maintained by RSC saving hundreds of thousands of dollars. d. Collaboration with DPI ETB members and Arimation Robotics to help design, build and implement an automation-friendly instrument to measure and collect data for Real Time Cellular Thermal Shift Assay (RT-CETSA). Design and testing phases complete with factory acceptance testing planned for September 2023. 2. Compound Management a. Automated tube reformatting and acoustic dispensing system designed, built custom for, and guided by NCATS compound management cutting edge needs. A straight from storage tube acoustic dispenser and a complex liquid handling platform was integrated with our compound store along with necessary peripherals for plate prep/preservation and decapping/capping capabilities to fully automated tube picking through assay ready plate creation while eliminating extra intermediate pipetting steps therefore reducing plastic consumables by 1/3 to 1/2 depending on the process. Installation, site acceptance testing, and training complete. Validation in process. b. Busiest year in history. 2,236 FOTS orders were filled for DPI biology groups and collaborators. Compounds requested 79% internally and 21% externally delivered around the country. c. 4,612 plates produced (acoustic 40% and non-acoustic 60%) for 771 orders. 65% were for Drug Combination, 27% for follow up plates, and 8% were qHTS style plates. 51% of the acoustic spotted plates were produced on our multiple automated acoustic platforms. 3. Automated Cell Technologies a. CellTrio System: RoboCell designed, custom built, and guided by NCATS cell technologies cutting edge needs to increase capability as well as scale up from existing Sartorius CompacT SelecT units. Includes flask handling, decappng/capping, detector, confluency inspection/imaging, complex liquid handling and dispensing as well as storage. Factory acceptance testing is ongoing to ensure necessary functionality. b. Five robotic high throughput biomanufacturing and functional differentiation processes of hiPSCs for Nocicenptors, Astrocytes, Trophoblasts, Hypothalamic neurons as well as adaptation of patient iPSCs in system and clean up. Supports internal (Stem Cell, 3DTBPL, etc) and numerous external collaborators. Involves direct involvement and feedback from scientists on process and evaluation. c. Nociceptors cell bank generated from 12 iPSC lines into 792 cryovials containing 5M cells/vial = 3.96B Nociceptor cells. Collaborators include Boston Childrens Hospital, Harvard Medical School, Department of Neurobiology, Dana Farber Cancer Institute, Department of Cancer Biology, and Nuffield Department of Clinical Neurosciences, The University of Oxford. Astrocytes cell bank generated from 14 iPSC lines into 392 cryovials containing 0.4M 10M cells/vial = 2.5B Astrocyte cells. Hypothalamic neurons generated from 3 iPSC lines into 91 cryovials containing 5M cells/vial = 455M Hypothalamic neurons.