Molecular imaging of viral infections

进度： SARS-CoV-2项目： 我们已经开始了我们的实验，通过气管内注射脂多糖来建立脂多糖肺损伤模型。我们使用的是雄性和雌性小鼠，包括WT和ACE2转基因小鼠。在这个阶段，我们正在对肺浸润物进行成像，并通过Western和实时定量定量聚合酶链式反应评估不同组的ACE2水平。我们希望在几个月内对所有实验进行详细的分析。 HIV项目：我们正在优化我们的动物模型，将表达gp120的HEK293肿瘤移植到裸鼠体内，与野生型肿瘤进行比较。我们已经用18F验证和标记了多个针对GP120的纳米体，并开始了PET成像。结果尚待公布。 埃博拉和其他高后果病毒感染：我们正在与综合研究机构合作，进行多项研究，评估尼帕、拉萨、马尔堡、新冠肺炎和埃博拉感染。

Progress: Sars-Cov-2 project: We have started our experiments by developing the LPS lung injury model by administering LPS intratracheally. We are using male and female mice, including WT and ACE2 Transgenic mice. At this stage we are imaging the lung infiltrates and assessing ACE2 levels in the various groups by western and real time qPCR. We are hoping to perform a detailed analysis of all the experiments in a few months. HIV project: We are in the stage of optimizing our animal model of Gp120 expressing HEK293 tumors implanted in nude mice in comparison to wild type tumors. We have validated and labeled multiple nanobodies against GP120 with 18F and started PET imaging. Results are pending. Ebola and other high consequence viral infection: We are collaborating with the Integrated research facility on multiple studies evaluating Nipah, Lassa, Marburg, Covid-19 and Ebola infections.